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MAP9 (NM_001039580) Human Tagged ORF Clone

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SKU
RC222565
MAP9 (Myc-DDK-tagged)-Human microtubule-associated protein 9 (MAP9)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $602.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP9
Synonyms ASAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222565 representing NM_001039580
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGATGAAGTTTTTAGCACCACTTTGGCATATACAAAGAGTCCAAAAGTTACCAAAAGAACTACTT
TCCAGGATGAGCTAATAAGAGCAATTACAGCTCGCTCAGCCAGACAAAGGAGTTCTGAATACTCAGATGA
CTTTGACAGTGATGAGATTGTTTCTTTAGGTGATTTTTCTGACACTTCAGCAGATGAAAATTCAGTTAAT
AAAAAAATGAATGACTTTCATATATCAGATGATGAAGAAAAGAATCCTTCAAAACTATTGTTTTTGAAAA
CCAATAAATCAAACGGTAACATAACCAAAGATGAGCCAGTGTGTGCCATCAAAAATGAAGAGGAAATGGC
ACCTGATGGGTGTGAAGACATTGTTGTAAAATCTTTCTCTGAATCTCAAAATAAGGATGAGGAATTTGAA
AAAGACAAAATAAAAATGAAACCTAAACCCAGAATTCTTTCAATTAAAAGCACATCTTCAGCAGAAAACA
ACAGCCTTGACACAGATGATCACTTTAAACCATCACCTCGGCCAAGGAGTATGTTGAAAAAGAAAAGTCA
CATGGAGGAGAAGGATGGACTAGAAGATAAAGAAACTGCCCTCAGTGAAGAATTGGAGTTACATTCTGCA
CCTTCTTCCCTTCCAACGCCGAATGGCATACAATTAGAAGCTGAGAAAAAAGCATTCTCTGAAAACCTTG
ATCCTGAGGATTCATGCTTAACAAGTCTAGCATCATCATCACTTAAACAAATTCTTGGAGATTCTTTTTC
ACCAGGATCTGAGGGAAACGCATCTGGAAAAGATCCAAATGAAGAAATCACTGAAAACCATAATTCCTTG
AAATCAGATGAAAATAAAGAGAATTCATTTTCAGCAGACCATGTGACTACTGCAGTTGAGAAATCCAAGG
AAAGTCAAGTGACTGCTGATGACCTTGAAGAAGAAAAGGCAAAAGCGGAACTGATTATGGATGATGACAG
AACAGTTGATCCACTACTATCTAAATCTCAGAGTATCTTAATATCTACCAGTGCAACAGCATCTTCAAAG
AAAACAATTGAAGATAGAAATATAAAGAATAAAAAGTCAACAAATAATAGAGCATCCAGTGCATCTGCCA
GATTAATGACCTCTGAGTTTTTGAAGAAATCTAGTTCTAAAAGGAGAACTCCATCGACAACTACCTCTTC
TCACTATTTAGGGACTTTAAAAGTCTTGGACCAAAAACCTTCACAGAAACAGAGCATAGAACCTGATAGA
GCAGATAACATAAGGGCAGCTGTTTATCAGGAGTGGTTAGAAAAGAAAAATGTGTATTTACATGAAATGC
ACAGAATAAAAAGAATTGAAAGTGAAAACTTAAGGATCCAAAATGAACAGAAAAAAGCTGCTAAAAGAGA
AGAAGCATTAGCATCATTTGAGGCCTGGAAGGCTATGAAAGAAAAGGAAGCAAAGAAAATAGCTGCCAAA
AAGAGGCTTGAAGAAAAAAACAAGAAGAAAACTGAAGAAGAAAATGCTGCAAGAAAAGGAGAAGCACTAC
AAGCTTTTGAAAAATGGAAAGAGAAAAAGATGGAATATCTTAAAGAGAAAAATAGAAAGGAGAGAGAATA
TGAAAGAGCAAAGAAACAGAAAGAGGAGGAAACTGTTGCCGAGAAAAAGAAAGATAATTTAACTGCTGTT
GAGAAATGGAATGAAAAAAAGGAAGCTTTTTTCAAGCAAAAGGAAAAAGAAAAAATAAATGAGAAAAGAA
AGGAAGAACTGAAAAGAGCTGAGAAAAAAGATAAAGATAAACAAGCTATTAATGAATATGAAAAATGGCT
GGAAAATAAGGAAAAACAAGAAAGAATTGAACGAAAACAGAAGAAACGTCATTCCTTTCTTGAAAGTGAG
GCACTTCCTCCGTGGAGCCCTCCAAGCAGAACTGTGTTCGCAAAAGTGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222565 representing NM_001039580
Red=Cloning site Green=Tags(s)

MSDEVFSTTLAYTKSPKVTKRTTFQDELIRAITARSARQRSSEYSDDFDSDEIVSLGDFSDTSADENSVN
KKMNDFHISDDEEKNPSKLLFLKTNKSNGNITKDEPVCAIKNEEEMAPDGCEDIVVKSFSESQNKDEEFE
KDKIKMKPKPRILSIKSTSSAENNSLDTDDHFKPSPRPRSMLKKKSHMEEKDGLEDKETALSEELELHSA
PSSLPTPNGIQLEAEKKAFSENLDPEDSCLTSLASSSLKQILGDSFSPGSEGNASGKDPNEEITENHNSL
KSDENKENSFSADHVTTAVEKSKESQVTADDLEEEKAKAELIMDDDRTVDPLLSKSQSILISTSATASSK
KTIEDRNIKNKKSTNNRASSASARLMTSEFLKKSSSKRRTPSTTTSSHYLGTLKVLDQKPSQKQSIEPDR
ADNIRAAVYQEWLEKKNVYLHEMHRIKRIESENLRIQNEQKKAAKREEALASFEAWKAMKEKEAKKIAAK
KRLEEKNKKKTEEENAARKGEALQAFEKWKEKKMEYLKEKNRKEREYERAKKQKEEETVAEKKKDNLTAV
EKWNEKKEAFFKQKEKEKINEKRKEELKRAEKKDKDKQAINEYEKWLENKEKQERIERKQKKRHSFLESE
ALPPWSPPSRTVFAKVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039580
ORF Size 1941 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039580.2
RefSeq Size 7333 bp
RefSeq ORF 1944 bp
Locus ID 79884
UniProt ID Q49MG5, Q05CN5
Cytogenetics 4q32.1
MW 74.1 kDa
Summary ASAP is a microtubule-associated protein required for spindle function, mitotic progression, and cytokinesis (Saffin et al., 2005 PubMed 16049101).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_001039580" in other vectors (4)
SKU Description Size Price
RC222565L3 Lenti ORF clone of Human microtubule-associated protein 9 (MAP9), Myc-DDK-tagged 10 ug
$902.00
RC222565L4 Lenti ORF clone of Human microtubule-associated protein 9 (MAP9), mGFP tagged 10 ug
$902.00
RG222565 MAP9 (tGFP-tagged) - Human microtubule-associated protein 9 (MAP9) 10 ug
$802.00
SC310781 MAP9 (untagged)-Human microtubule-associated protein 9 (MAP9) 10 ug
$603.00

Plasmid Map for RC222565

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222565
Circular map for RC222565

file-alt Citations

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