TMEM95 (NM_198154) Human Tagged ORF Clone

  • TrueORF®
SKU
RC222524
TMEM95 (Myc-DDK-tagged)-Human transmembrane protein 95 (TMEM95)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM95
Synonyms UNQ9390
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222524 representing NM_198154
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGGCTGGCACTAGGCGGGGTTTTCCTGGCAGCCGCCCAGGCTTGTGTCTTCTGTCGCCTCCCAG
CCCACGACTTGTCAGGCCGCCTGGCTCGGCTCTGCAGCCAGATGGAGGCCAGGCAGAAGGAATGTGGGGC
CTCCCCAGACTTCTCGGCCTTTGCCTTAGATGAGGTGTCCATGAACAAAGTCACAGAGAAGACTCACAGA
GTCCTGAGGGTCATGGAAATCAAAGAGGCTGTCTCCTCACTCCCTTCATATTGGAGTTGGCTTCGAAAGA
CCAAGCTCCCTGAGTACACCAGGGAAGCTCTCTGTCCCCCCGCCTGCCCGTTGCCTCTGCTGTCTCCTGC
AGGGGGCAGCACCACGCTGTACAACTGCTCCACCTGCAAGGGGACGGAGGTGTCCTGCTGGCCCCGAAAG
CGCTGCTTCCCAGGAAGTCAGGATCTTTGGGAAGCCAAGATTCTGCTCCTCTCCATCTTCGGAGCTTTCC
TGCTTCTGGGTGTTCTGAGCCTCCTGGTGGAGTCCCACCACCTCCAAGCAAAAAGTGGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222524 representing NM_198154
Red=Cloning site Green=Tags(s)

MWRLALGGVFLAAAQACVFCRLPAHDLSGRLARLCSQMEARQKECGASPDFSAFALDEVSMNKVTEKTHR
VLRVMEIKEAVSSLPSYWSWLRKTKLPEYTREALCPPACPLPLLSPAGGSTTLYNCSTCKGTEVSCWPRK
RCFPGSQDLWEAKILLLSIFGAFLLLGVLSLLVESHHLQAKSGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198154
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198154.3
RefSeq Size 1169 bp
RefSeq ORF 555 bp
Locus ID 339168
UniProt ID Q3KNT9
Cytogenetics 17p13.1
MW 20.1 kDa
Summary Sperm protein required for fusion of sperm with the egg membrane during fertilization.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198154" in other vectors (4)
SKU Description Size Price
RC222524L3 Lenti-ORF clone of TMEM95 (Myc-DDK-tagged)-Human transmembrane protein 95 (TMEM95) 10 ug
$600.00
RC222524L4 Lenti-ORF clone of TMEM95 (mGFP-tagged)-Human transmembrane protein 95 (TMEM95) 10 ug
$600.00
RG222524 TMEM95 (tGFP-tagged) - Human transmembrane protein 95 (TMEM95) 10 ug
$500.00
SC307636 TMEM95 (untagged)-Human transmembrane protein 95 (TMEM95) 10 ug
$330.00

Plasmid Map for RC222524

Download sequence for

Cloning scheme for RC222524

Circular map for RC222524

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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