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PRR25 (NM_001013638) Human Tagged ORF Clone

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SKU
RC222479
PRR25 (Myc-DDK-tagged)-Human proline rich 25 (PRR25)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRR25
Synonyms gs64
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222479 representing NM_001013638
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGAACTGACCAGAAGCCCCCGTGCAGAGGTGGCTGCTGGGGACAGCCAGGTCACCCCAACACGG
GTGGGGCAGCTGCACACCCCACTTACCACCCCATGGGTCACAGGCCTCGAACGTGCATCCTGCTACGTGG
AGACCAGACCACAGGTGGCCAAGCTCCGTCTCGGGAAATAAGCCTGGGGCCCTGGGCGGCCGGCACTCAC
TTCCTGGCTATCTCTACCACCCCATGGGGCAGGAAGACACCCGCCTGCATTTCTGAGCTCCCAACCAGCT
CAGGAACAGCGCAGCCGCTCGCAAATGCAGTGTGCGAGGTACAGACCGTTCCAGGGCCTGGCCTGCGCCC
ACAGGGAACTCCTGCCATGCGGGCCCCTTCTCACAAGGGGACACCGCCCACCCCCAACCCCTGGGGCCCA
GAGCAGCCCCAGAACAGACACAAGCATCCCAAAAAGGGGGTCACTGGGGGCCCCTCGCCGCCGCCTCCTG
CCGCCTCCCGATATGGCCAAACACCCGGGCGCGAGCCCCGCGTGCAGGCGCCGGGCCTGGGCCCCTGTGG
CCGTCCCGCCAGCGGCCGTCTTCTGTCCCTGCATTTAGAGAAGGGGGATGGCAAAGGGACCCGCCAGCGG
ATCCCTCTTACAGATGCTGCCGTGGGCGGCGACCGCACAGACATCCCCTCTGCTATTGCTGCGGGACCGG
CAAGGACGCCGGACCGACACGGCCTCCCCATCCCTGGGTCCACCCCGACTCCCATGGTGGGGAGCGGCCG
GCTTGGAGCCCCTGTGGGACGGAGTGGAGGTGGAGCAAGTGCCCGGAGCTCTCGGCCATCATGTGCGAAT
GTTTTGCTCCGTGCTGACGCCTCCCTCGGCACCGTCCTGTCTGTGTTGTGGACTGGGCAGCTCTCACGGG
GTTGGGCGCTTCTGCCCCCGGGGGACGCTGGCCGGCATCTGGAGACGTCTGTGATCTCCGCTGGTGTTGC
CGCAGGCATCTGGCTGGTGGAGCCCGGGGAGGCCGCCCAGGACCCTGCGACCCGCAGGACCGCCCCGCCG
CGGAGGACGGCCAGCCCCGAACCCCCAGCGCCCGGGGCACCCCTGCCTGCCTGCCCTGGGCGGATCCCCG
GGGCCGCGCGGTTCGGGCCGCGCTCCTGCCCGCTGGGGTCTCCCGCCGTCCTCGCCGTCACCACCGGGTG
GAGCCACAGGTCCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222479 representing NM_001013638
Red=Cloning site Green=Tags(s)

MARTDQKPPCRGGCWGQPGHPNTGGAAAHPTYHPMGHRPRTCILLRGDQTTGGQAPSREISLGPWAAGTH
FLAISTTPWGRKTPACISELPTSSGTAQPLANAVCEVQTVPGPGLRPQGTPAMRAPSHKGTPPTPNPWGP
EQPQNRHKHPKKGVTGGPSPPPPAASRYGQTPGREPRVQAPGLGPCGRPASGRLLSLHLEKGDGKGTRQR
IPLTDAAVGGDRTDIPSAIAAGPARTPDRHGLPIPGSTPTPMVGSGRLGAPVGRSGGGASARSSRPSCAN
VLLRADASLGTVLSVLWTGQLSRGWALLPPGDAGRHLETSVISAGVAAGIWLVEPGEAAQDPATRRTAPP
RRTASPEPPAPGAPLPACPGRIPGAARFGPRSCPLGSPAVLAVTTGWSHRSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001013638
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001013638.1, NP_001013660.1
RefSeq Size 1209 bp
RefSeq ORF 1209 bp
Locus ID 388199
UniProt ID Q96S07
Cytogenetics 16p13.3
MW 40.8 kDa
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"NM_001013638" in other vectors (4)
SKU Description Size Price
RC222479L3 Lenti ORF clone of Human proline rich 25 (PRR25), Myc-DDK-tagged 10 ug
$757.00
RC222479L4 Lenti ORF clone of Human proline rich 25 (PRR25), mGFP tagged 10 ug
$757.00
RG222479 PRR25 (tGFP-tagged) - Human proline rich 25 (PRR25) 10 ug
$657.00
SC301813 PRR25 (untagged)-Human proline rich 25 (PRR25) 10 ug
$503.00

Plasmid Map for RC222479

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222479
Circular map for RC222479

file-alt Citations

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