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DYRK1B (NM_006483) Human Tagged ORF Clone

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SKU
RC222376
DYRK1B (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant b
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $548.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "DYRK1B or NM_006483"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DYRK1B
Synonyms AOMS3; MIRK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222376 representing NM_006483
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACGCAGGTATTGC
CTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTCCGGGATGCAACCTCAGCCCCGCTGCGTAA
GCTCTCTGTGGACCTCATCAAGACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGG
GCCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGTCCTGAACCATGGTTATGATG
ACGACAACCATGACTACATCGTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCAT
TGGCAAAGGCTCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTGGCCATCAAG
ATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTGATGAACC
AGCATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTG
CCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACACCCACTTCCGCGGCGTCTCG
CTGAACCTGACCCGGAAGCTGGCGCAGCAGCTCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCA
GCATCATTCACTGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCGCCATCAAGAT
TGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGCCGCTTCTACCGC
TCACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTTG
TGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGACCAGATGAACCGCATTGTGGA
GGTGCTGGGCATCCCACCGGCCGCCATGCTGGACCAGGCGCCCAAGGCTCGCAAGTACTTTGAACGGCTG
CCTGGGGGTGGCTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGACCTGGTGCTGCGCATGCTGGAGT
ATGAGCCCGCCGCCCGCATCAGCCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGA
GGCCACCAACACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCT
TCCAGCACCGCCAGCTCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTCCAGTGACAACCGGACCTACC
GCTACAGCAACCGATATTGTGGGGGCCCTGGGCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGT
CCCACCCTCCCAGCCGCTGCGGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCAAGCCCCTGCC
TCTGCCTCGTCACTGCCTGGGACCGGGGCCCAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCAT
CACCAACCTCACCACCACCCCCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCC
ACCTCACCCAGCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGT
CCACCCCTCCCGCCTCCTGATGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGA
GCACAGCAGCCAGCTCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222376 representing NM_006483
Red=Cloning site Green=Tags(s)

MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRR
AQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIK
IIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVS
LNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYR
SPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERL
PGGGWTLRRTKELRKDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPS
SSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPA
SASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGR
PPLPPPDDPATLGPHLGLRGVPQSTAASS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006483
ORF Size 1767 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006483.3
RefSeq Size 2420 bp
RefSeq ORF 1770 bp
Locus ID 9149
UniProt ID Q9Y463, A0A024R0J6, Q9Y463-2
Cytogenetics 19q13.2
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
MW 64.8 kDa
Summary This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2014
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Documents
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"NM_006483" in other vectors (4)
SKU Description Size Price
RC222376L3 Lenti-ORF clone of DYRK1B (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant b 10 ug
$848.00
RC222376L4 Lenti-ORF clone of DYRK1B (mGFP-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant b 10 ug
$848.00
RG222376 DYRK1B (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant b 10 ug
$748.00
SC310235 DYRK1B (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant b 10 ug
$604.00

Plasmid Map for RC222376

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222376
Circular map for RC222376

file-alt Citations

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