keratin 40 (KRT40) (NM_182497) Human Tagged ORF Clone

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SKU
RC222348
KRT40 (Myc-DDK-tagged)-Human keratin 40 (KRT40)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol keratin 40
Synonyms CK-40; K40; KA36
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222348 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCTGACTGCTCCTCCACACACTGCTCTCCTGAGTCCTGTGGCACGGCTTCCGGTTGTGCACCTG
CCTCAAGCTGCTCCGTGGAAACAGCTTGTCTCCCCGGTACCTGTGCTACATCCCGATGTCAGACTCCAAG
CTTCCTATCCAGGTCTCGCGGGCTGACTGGTTGCCTCCTGCCATGCTACTTTACTGGGAGTTGTAATAGT
CCCTGCTTGGTGGGGAACTGTGCCTGGTGTGAGGATGGGGTGTTCACTAGCAATGAGAAGGAGACGATGC
AGTTCCTGAATGACAGACTCGCCAGCTATCTGGAGAAGGTGCGCAGCCTGGAGGAGACCAACGCAGAGCT
GGAATCCAGGATCCAAGAACAATGTGAACAGGATATCCCAATGGTGTGCCCGGATTATCAGCGTTACTTC
AACACCATTGAAGATCTCCAACAAAAGATCTTATGCACGAAAGCAGAGAATTCTAGACTTGCTGTACAGC
TTGACAACTGCAAACTGGCCACTGATGACTTTAAGTCAAAGTACGAGAGTGAACTGTCCCTTCGCCAGCT
GTTAGAGGCTGACATCAGCAGCCTGCATGGGATCCTGGAGGAACTGACCCTGTGCAAATCTGATCTGGAG
GCCCATGTGGAGTCTCTGAAGGAAGATCTCCTTTGCCTTAAGAAAAACCATGAAGAGGAAGTCAACTTGC
TTCGTGAACAGCTTGGCGACCGCCTCAGTGTGGAGCTGGACACTGCCCCCACCCTTGACCTCAACAGGGT
CCTGGATGAGATGCGCTGTCAGTGTGAAACGGTGCTTGCCAACAATCGCAGAGAAGCTGAAGAATGGTTG
GCTGTTCAGACAGAAGAGCTGAATCAGCAGCAACTGTCCAGCGCGGAGCAGCTGCAGGGCTGCCAGATGG
AGATCTTGGAACTGAAACGCACAGCCAGTGCTCTGGAAATTGAGCTCCAAGCACAGCAAAGCCTGACAGA
ATCTCTGGAATGCACCGTGGCAGAAACCGAGGCCCAGTACAGCTCCCAGCTGGCCCAAATTCAGTGTCTG
ATCGATAACCTGGAGAACCAGCTGGCCGAGATCCGCTGCGACCTGGAGCGACAGAACCAGGAGTACCAGG
TGCTCCTGGACGTGAAGGCCCGGCTGGAGGGTGAGATCAACACGTACTGGGGCCTGCTGGACAGCGAGGA
CAGCAGGCTTTCCTGTAGCCCATGTTCGACCACATGCACCTCGAGCAACACTTGTGAGCCATGCTCAGCC
TATGTCATCTGCACTGTTGAAAACTGCTGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222348 protein sequence
Red=Cloning site Green=Tags(s)

MTSDCSSTHCSPESCGTASGCAPASSCSVETACLPGTCATSRCQTPSFLSRSRGLTGCLLPCYFTGSCNS
PCLVGNCAWCEDGVFTSNEKETMQFLNDRLASYLEKVRSLEETNAELESRIQEQCEQDIPMVCPDYQRYF
NTIEDLQQKILCTKAENSRLAVQLDNCKLATDDFKSKYESELSLRQLLEADISSLHGILEELTLCKSDLE
AHVESLKEDLLCLKKNHEEEVNLLREQLGDRLSVELDTAPTLDLNRVLDEMRCQCETVLANNRREAEEWL
AVQTEELNQQQLSSAEQLQGCQMEILELKRTASALEIELQAQQSLTESLECTVAETEAQYSSQLAQIQCL
IDNLENQLAEIRCDLERQNQEYQVLLDVKARLEGEINTYWGLLDSEDSRLSCSPCSTTCTSSNTCEPCSA
YVICTVENCCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182497
ORF Size 1293 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182497.4
RefSeq Size 1938 bp
RefSeq ORF 1296 bp
Locus ID 125115
UniProt ID Q6A162
Cytogenetics 17q21.2
MW 48.1 kDa
Summary This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. provided by RefSeq, Jul 2009
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Documents
Resources
"NM_182497" in other vectors (4)
SKU Description Size Price
RC222348L3 Lenti ORF clone of Human keratin 40 (KRT40), Myc-DDK-tagged 10 ug
$757.00
RC222348L4 Lenti ORF clone of Human keratin 40 (KRT40), mGFP tagged 10 ug
$757.00
RG222348 KRT40 (tGFP-tagged) - Human keratin 40 (KRT40) 10 ug
$489.00 $657.00
SC317695 KRT40 (untagged)-Human keratin 40 (KRT40) 10 ug
$503.00

Plasmid Map for RC222348

Download sequence for

Cloning scheme for RC222348

Circular map for RC222348

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