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PTPDC1 (NM_152422) Human Tagged ORF Clone

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SKU
RC222339
PTPDC1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase domain containing 1 (PTPDC1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $738.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTPDC1
Synonyms PTP9Q22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222339 representing NM_152422
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGTGCAGGATGCAACCAGGCGGCCCTCAGCCGTGCGCTTCCTCAGCTCCTTTCTCCAGGGCCGCC
GGCACTCCACCTCAGACCCAGTACTGCGGCTGCAGCAGGCCCGGCGGGGCTCTGGCTTGGGCTCCGGCTC
TGCCACGAAGCTGCTGTCCTCGTCCTCTCTCCAGGTGATGGTGGCTGTTTCCTCAGTCAGCCATGCAGAG
GGAAACCCAACTTTCCCCGAAAGAAAAAGAAATTTAGAACGTCCAACACCAAAGTACACAAAAGTAGGGG
AGCGTTTACGGCATGTCATTCCTGGACACATGGCATGTTCCATGGCGTGTGGCGGTAGAGCTTGCAAGTA
TGAGAACCCAGCCCGCTGGAGTGAGCAGGAGCAAGCCATTAAGGGGGTTTACTCATCCTGGGTCACTGAT
AATATACTGGCCATGGCCCGCCCATCCTCTGAGCTCCTGGAGAAGTACCACATCATTGATCAGTTCCTCA
GCCATGGCATAAAAACAATAATCAACCTCCAGCGCCCTGGTGAGCATGCTAGCTGTGGGAACCCTCTGGA
ACAAGAAAGTGGCTTCACATACCTTCCTGAGGCTTTCATGGAGGCTGGCATTTACTTCTACAATTTCGGA
TGGAAGGATTATGGTGTAGCGTCTCTTACTACTATCCTAGATATGGTGAAGGTGATGACATTTGCCTTAC
AGGAAGGAAAAGTAGCTATCCATTGTCATGCAGGGCTTGGTCGAACAGGTGTTTTAATAGCCTGTTACTT
AGTTTTTGCAACGAGAATGACTGCTGACCAAGCAATTATATTTGTGCGGGCAAAGCGACCCAATTCCATA
CAAACCAGAGGACAGCTCCTCTGTGTAAGGGAATTTACTCAGTTTCTAACTCCTCTCCGCAATATATTCT
CTTGCTGTGATCCCAAAGCACATGCTGTCACCTTACCTCAATATCTAATTCGCCAGCGTCATCTGCTTCA
TGGTTATGAGGCACGACTTCTGAAACACGTGCCAAAAATTATCCACCTAGTTTGCAAATTGCTGCTGGAC
TTAGCGGAGAACAGGCCAGTGATGATGAAGGATGTGTCCGAAGGACCTGGTCTCTCTGCTGAAATAGAAA
AGACAATGTCTGAGATGGTCACCATGCAGCTGGATAAAGAGTTACTGAGGCATGACAGTGATGTGTCCAA
CCCGCCTAACCCCACTGCAGTGGCAGCAGATTTTGACAATCGAGGCATGATTTTCTCCAATGAGCAACAG
TTTGACCCTCTTTGGAAAAGGCGGAATGTTGAGTGCCTTCAACCCCTGACTCATCTGAAAAGGCGGCTCA
GCTACAGTGACTCAGATTTAAAGAGGGCCGAGAACCTCCTGGAGCAAGGGGAGACTCCACAGACAGTGCC
TGCCCAGATCTTGGTTGGCCACAAGCCCAGGCAGCAGAAGCTCATAAGCCATTGTTACATCCCACAGTCT
CCAGAACCAGACTTACACAAGGAAGCCTTGGTTCGCAGCACACTTTCTTTCTGGAGTCAGTCAAAGTTTG
GAGGCCTGGAAGGACTCAAAGATAATGGGTCACCAATTTTCCATGGAAGGATCATTCCAAAGGAAGCACA
GCAGAGTGGAGCTTTCTCTGCAGATGTTTCAGGCTCACACAGCCCTGGGGAGCCAGTTTCACCCAGCTTT
GCAAATGTCCATAAGGATCCAAACCCTGCTCACCAGCAAGTGTCTCACTGTCAGTGTAAAACTCATGGTG
TTGGGAGCCCTGGCTCTGTCAGGCAGAACAGCAGGACACCCCGAAGCCCTCTGGACTGTGGCTCCAGTCC
CAAAGCACAGTTCTTGGTTGAACATGAAACCCAGGACAGTAAAGATCTGTCTGAAGCAGCTTCACACTCT
GCATTACAGTCTGAATTGAGTGCTGAGGCAAGAAGAATACTGGCGGCCAAAGCCCTAGCAAATTTAAATG
AATCTGTAGAAAAGGAGGAACTAAAAAGGAAGGTAGAAATGTGGCAGAAAGAGCTTAATTCCCGAGATGG
AGCTTGGGAAAGAATATGTGGCGAGAGGGACCCTTTCATCCTATGCAGCTTGATGTGGTCTTGGGTGGAG
CAACTGAAGGAGCCTGTAATCACCAAAGAGGATGTGGACATGTTGGTTGACAGGCGAGCAGATGCCGCAG
AAGCACTTTTTTTATTAGAGAAGGGACAGCACCAGACTATTCTCTGCGTGTTGCACTGCATAGTGAACCT
GCAGACAATTCCCGTGGATGTGGAGGAAGCTTTCCTTGCCCATGCCATTAAGGCATTCACTAAGGTTAAT
TTTGATTCTGAAAATGGACCAACAGTTTACAACACCCTGAAGAAAATATTTAAGCACACGCTGGAAGAAA
AAAGAAAAATGACAAAAGATGGCCCTAAGCCTGGCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222339 representing NM_152422
Red=Cloning site Green=Tags(s)

MQVQDATRRPSAVRFLSSFLQGRRHSTSDPVLRLQQARRGSGLGSGSATKLLSSSSLQVMVAVSSVSHAE
GNPTFPERKRNLERPTPKYTKVGERLRHVIPGHMACSMACGGRACKYENPARWSEQEQAIKGVYSSWVTD
NILAMARPSSELLEKYHIIDQFLSHGIKTIINLQRPGEHASCGNPLEQESGFTYLPEAFMEAGIYFYNFG
WKDYGVASLTTILDMVKVMTFALQEGKVAIHCHAGLGRTGVLIACYLVFATRMTADQAIIFVRAKRPNSI
QTRGQLLCVREFTQFLTPLRNIFSCCDPKAHAVTLPQYLIRQRHLLHGYEARLLKHVPKIIHLVCKLLLD
LAENRPVMMKDVSEGPGLSAEIEKTMSEMVTMQLDKELLRHDSDVSNPPNPTAVAADFDNRGMIFSNEQQ
FDPLWKRRNVECLQPLTHLKRRLSYSDSDLKRAENLLEQGETPQTVPAQILVGHKPRQQKLISHCYIPQS
PEPDLHKEALVRSTLSFWSQSKFGGLEGLKDNGSPIFHGRIIPKEAQQSGAFSADVSGSHSPGEPVSPSF
ANVHKDPNPAHQQVSHCQCKTHGVGSPGSVRQNSRTPRSPLDCGSSPKAQFLVEHETQDSKDLSEAASHS
ALQSELSAEARRILAAKALANLNESVEKEELKRKVEMWQKELNSRDGAWERICGERDPFILCSLMWSWVE
QLKEPVITKEDVDMLVDRRADAAEALFLLEKGQHQTILCVLHCIVNLQTIPVDVEEAFLAHAIKAFTKVN
FDSENGPTVYNTLKKIFKHTLEEKRKMTKDGPKPGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152422
ORF Size 2418 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152422.4
RefSeq Size 4398 bp
RefSeq ORF 2421 bp
Locus ID 138639
UniProt ID A2A3K4
Cytogenetics 9q22.32
Domains DSPc, PTPc_motif, Y_phosphatase
Protein Families Druggable Genome, Phosphatase
MW 90 kDa
Summary The protein encoded by this gene contains a characteristic motif of protein tyrosine phosphatases (PTPs). PTPs regulate activities of phosphoproteins through dephosphorylation. They are signaling molecules involved in the regulation of a wide variety of biological processes. The specific function of this protein has not yet been determined. Alternatively spliced transcript variants encoding distinct isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_152422" in other vectors (4)
SKU Description Size Price
RC222339L3 Lenti ORF clone of Human protein tyrosine phosphatase domain containing 1 (PTPDC1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,038.00
RC222339L4 Lenti ORF clone of Human protein tyrosine phosphatase domain containing 1 (PTPDC1), transcript variant 1, mGFP tagged 10 ug
$1,038.00
RG222339 PTPDC1 (tGFP-tagged) - Human protein tyrosine phosphatase domain containing 1 (PTPDC1), transcript variant 1 10 ug
$789.00 $938.00
SC128129 PTPDC1 (untagged)-Human protein tyrosine phosphatase domain containing 1 (PTPDC1), transcript variant 1 10 ug
$739.00

Plasmid Map for RC222339

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222339
Circular map for RC222339

file-alt Citations

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