OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SH2B1 (NM_015503) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC222333
SH2B1 (Myc-DDK-tagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $614.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA333813 Rabbit Polyclonal Anti-SH2B1 Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "SH2B1 or NM_015503"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SH2B1
Synonyms PSM; SH2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222333 representing NM_015503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGTGCCCCTTCCCCAGAGGACGGGGCCTCCCCCTCGTCTCCCCCGCTGCCCCCACCCCCGCCCC
CTAGTTGGCGGGAGTTCTGTGAGTCCCACGCCCGGGCTGCGGCTCTGGACTTTGCCCGCCGTTTTCGCCT
CTACCTGGCCTCCCACCCCCAATATGCGGGGCCCGGGGCCGAGGCTGCCTTCTCCCGCCGTTTTGCTGAG
CTCTTCCTGCAGCACTTTGAAGCCGAGGTGGCCCGGGCCTCTGGCTCCCTGTCGCCACCCATCCTGGCTC
CCCTGAGCCCTGGTGCGGAGATTTCGCCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCCCCT
GGCTGTGCTGGGCCCTTCTCGATCATCTGAGGACCTGGCCGGCCCCCTCCCTTCCTCAGTCTCTTCCTCC
TCTACAACCTCCTCCAAGCCGAAGCTCAAGAAGCGCTTTTCCCTGCGTTCAGTGGGTCGCTCTGTCCGAG
GCTCAGTCCGTGGCATCCTGCAGTGGCGGGGGACCGTTGACCCTCCCTCCTCCGCTGGGCCCCTGGAGAC
CTCGTCAGGCCCCCCAGTCTTAGGTGGAAACAGCAACTCCAACTCCTCTGGCGGGGCTGGGACCGTTGGT
AGGGGACTGGTCAGTGATGGAACGTCCCCTGGGGAAAGATGGACTCACCGTTTTGAGAGGCTGAGACTCA
GTCGGGGAGGGGGCGCCTTGAAGGATGGAGCAGGGATGGTGCAGAGGGAAGAGCTGCTGAGTTTCATGGG
GGCTGAGGAGGCAGCCCCTGACCCAGCCGGAGTGGGCCGGGGAGGAGGGGTGGCTGGGCCTCCTTCAGGG
GGAGGAGGGCAGCCTCAGTGGCAGAAGTGTCGCCTGCTGCTTCGAAGTGAAGGAGAAGGAGGAGGAGGAA
GTCGCCTGGAGTTCTTTGTACCACCCAAGGCCTCTCGGCCCCGACTCAGCATCCCCTGCTCTTCTATCAC
AGACGTCCGGACAACCACAGCCCTGGAGATGCCTGACCGGGAGAACACGTTTGTGGTTAAGGTGGAAGGT
CCATCCGAGTATATCATGGAGACAGTGGATGCCCAGCATGTGAAGGCCTGGGTGTCTGACATCCAAGAAT
GCCTGAGCCCAGGACCCTGCCCTGCTACCAGTCCCCGCCCCATGACCCTCCCTCTGGCCCCTGGGACCTC
ATTCCTTACAAGGGAGAACACAGACAGCCTGGAGCTGTCCTGCCTGAATCACTCGGAGAGTCTACCCAGC
CAGGACCTGCTGCTTGGACCCAGCGAGAGCAATGACCGCCTGTCGCAGGGGGCATATGGGGGCCTCTCAG
ACCGCCCCTCGGCATCCATCTCCCCCAGCTCTGCCTCCATTGCCGCCTCCCATTTTGACTCGATGGAACT
GCTTCCCCCAGAGTTGCCCCCCCGCATCCCCATTGAAGAGGGACCCCCAACAGGGACAGTTCATCCCCTC
TCAGCCCCCTACCCTCCCTTGGACACTCCGGAAACAGCCACAGGGTCCTTCCTGTTCCAGGGGGAGCCAG
AGGGCGGTGAGGGGGACCAGCCCCTCTCAGGGTATCCTTGGTTCCACGGGATGCTCTCTCGGCTCAAGGC
TGCACAGTTGGTGCTGACTGGCGGCACTGGCTCCCACGGTGTCTTCCTGGTGCGCCAGAGTGAGACAAGG
CGGGGTGAATACGTCCTCACCTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGTTTGTCGCTGAACGAGG
AGGGTCAGTGCCGGGTCCAGCACCTGTGGTTCCAGTCCATTTTCGATATGCTCGAGCACTTCCGGGTGCA
CCCCATCCCTTTGGAGTCGGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTCCCATCCTCCCAGCGA
CAGCAGGGCCGGGAGCAGGCTGGGAGCCATGCGGGGGTGTGCGAGGGAGATGGATGCCACCCCGATGCCT
CCTGCACCCTCATGCCCTTCGGAGCGAGTGACTGTGTAACCGACCACCTCCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222333 representing NM_015503
Red=Cloning site Green=Tags(s)

MNGAPSPEDGASPSSPPLPPPPPPSWREFCESHARAAALDFARRFRLYLASHPQYAGPGAEAAFSRRFAE
LFLQHFEAEVARASGSLSPPILAPLSPGAEISPHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSSS
STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGTVDPPSSAGPLETSSGPPVLGGNSNSNSSGGAGTVG
RGLVSDGTSPGERWTHRFERLRLSRGGGALKDGAGMVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSG
GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEG
PSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPS
QDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPTGTVHPL
SAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLVLTGGTGSHGVFLVRQSETR
RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSSQR
QQGREQAGSHAGVCEGDGCHPDASCTLMPFGASDCVTDHLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015503
ORF Size 2013 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015503.2
RefSeq Size 4876 bp
RefSeq ORF 2016 bp
Locus ID 25970
UniProt ID Q9NRF2, A0A024QZC2
Cytogenetics 16p11.2
Protein Pathways Neurotrophin signaling pathway
MW 70.6 kDa
Summary This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. provided by RefSeq, Mar 2009
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_015503" in other vectors (4)
SKU Description Size Price
RC222333L3 Lenti-ORF clone of SH2B1 (Myc-DDK-tagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 2 10 ug
$914.00
RC222333L4 Lenti-ORF clone of SH2B1 (mGFP-tagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 2 10 ug
$914.00
RG222333 SH2B1 (tGFP-tagged) - Human SH2B adaptor protein 1 (SH2B1), transcript variant 2 10 ug
$814.00
SC304322 SH2B1 (untagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 2 10 ug
$615.00

Plasmid Map for RC222333

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222333
Circular map for RC222333

file-alt Citations

Crucial Role of the SH2B1 PH Domain for the Control of Energy Balance
Flores, Argetsinger, Stadler et al
Diabetes (2019)
AI Summary: OriGene contributed to this study by providing the human SH2B1β plasmid (pcDNA3.1(+), catalog #RC222333), which was used to generate and analyze SH2B1 mutations implicated in energy balance and glucose homeostasis.
Snippet: Page 1. 1 1 Crucial Role of the SH2B1 PH Domain for the Control of 2 Energy Balance 3 4 Anabel Floresa, Lawrence S. Argetsingerb+, Lukas KJ Stadlerc+, Alvaro E. Malagab, Paul B. 5 Vanderb, Lauren C. DeSantisb, Ray M;GFP-tagged rat SH2B1 (4) and SH2B1 P322S (5) (GenBank accession number NM_001048180) have been described previously. The PR deletion was introduced into GFPSH2B1 using a QuikChange II site-directed mutagenesis kit (Stratagene) and the deletion confirmed by sequencing. For Fig. 1B, human SH2B1 in pcDNA3.1(+) (GenBank accession number NM_015503) was purchased from Origene (# RC222333) and GFP from Addgene (plasmid no. 13031). Human SH2B1 was mutated using QuikChange II site-directed mutagenesis and the sequence confirmed by Sanger sequencing (3730 DNA Analyser, Thermo Scientific)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products