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UNCX (NM_001080461) Human Tagged ORF Clone

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SKU
RC222265
UNCX (Myc-DDK-tagged)-Human UNC homeobox (UNCX)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $494.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "UNCX or NM_001080461"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UNCX
Synonyms UNCX4.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222265 representing NM_001080461
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGACGGCCGCCTCCTGGAACACCCGCATGCCCAGTTCGGGGGCTCGCTGGGCGGCGTGGTGGGCT
TCCCCTACCCGCTGGGCCACCACCACGTGTACGAGCTGGCCGGGCACCAGCTGCAGTCGGCCGCCGCCGC
CGCCTCGGTGCCCTTCTCCATCGACGGCCTGCTCGGGGGCTCGTGCGCCGCCGCCGCCTCGGTGGTCAAC
CCCACGCCGCTGCTGCCAGCCGCCTGCGGGGTCGGCGGGGACGGCCAGCCCTTCAAGCTGTCAGACTCGG
GGGACCCGGACAAGGAGAGCCCGGGCTGCAAGCGGCGGCGCACCCGCACCAACTTCACCGGCTGGCAGCT
GGAGGAGCTGGAGAAGGCGTTCAACGAGAGCCACTATCCCGACGTGTTCATGCGCGAGGCGCTGGCGCTG
CGCCTAGACCTGGTCGAGTCCCGAGTTCAGGTCTGGTTCCAAAACCGCCGGGCCAAGTGGAGGAAGAAGG
AGAACACGAAAAAGGGCCCGGGGCGGCCGGCGCACAACTCGCACCCGACCACGTGCAGCGGCGAGCCCAT
GGACCCGGAGGAGATCGCGCGCAAGGAGCTGGAGAAGATGGAGAAGAAGAAGCGCAAGCACGAGAAGAAG
CTGCTGAAGAGCCAGGGCCGCCACTTGCACTCGCCCGGCGGCCTGTCCCTGCACAGCGCGCCCAGCTCCG
ACAGCGACAGCGGCGGCGGCGGCCTGTCTCCGGAGCCGCCCGAGCCGCCGCCGCCCGCCGCCAAGGGCCC
CGGAGCGCACGCCTCGGGCGCCGCGGGGACCGCGCCCGCCCCTCCCGGCGAGCCGCCTGCACCCGGCACC
TGCGACCCCGCCTTCTACCCGAGCCAAAGAAGCGGCGCCGGCCCACAGCCGCGCCCAGGTCGCCCTGCGG
ACAAGGACGCGGCCTCGTGCGGGCCAGGGGCCGCTGTGGCGGCGGTGGAGCGCGGCGCCGCGGGGCTGCC
CAAGGCCAGCCCATTCAGCGTGGAGAGCCTCCTGTCCGACTCGCCGCCGCGCCGGAAAGCCGCTTCCAAC
GCCGCCGCCGCCGCCGCCGCGGGGCTGGACTTCGCGCCCGGGCTGCCGTGCGCGCCGCGGACCCTGATCG
GCAAGGGCCACTTCCTCCTCTACCCCATCACGCAGCCGCTCGGCTTCCTGGTGCCGCAGGCCGCGCTCAA
GGGCGGCGCGGGCCTGGAGCCGGCGCCCAAGGACGCGCCGCCCGCGCCCGCCGTGCCGCCCGCGCCGCCT
GCCCAGGCCAGTTTCGGGGCCTTCTCGGGGCCCGGCGGCGCCCCGGACTCGGCCTTCGCGCGTCGCAGCC
CCGACGCCGTCGCCTCCCCGGGGGCCCCAGCCCCGGCCCCGGCGCCTTTCCGGGACCTCGCCTCGGCAGC
GGCTACCGAGGGCGGCGGCGGGGACTGCGCGGACGCGGGGACCGCCGGCCCCGCGCCCCCGCCGCCCGCG
CCGTCGCCCAGGCCCGGCCCTCGGCCTCCCAGCCCCGCCGAGGAGCCGGCCACCTGCGGGGTTCCCGAGC
CTGGCGCGGCGGCCGGACCCAGCCCGCCGGAGGGCGAGGAGCTGGACATGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222265 representing NM_001080461
Red=Cloning site Green=Tags(s)

MMDGRLLEHPHAQFGGSLGGVVGFPYPLGHHHVYELAGHQLQSAAAAASVPFSIDGLLGGSCAAAASVVN
PTPLLPAACGVGGDGQPFKLSDSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALAL
RLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDPEEIARKELEKMEKKKRKHEKK
LLKSQGRHLHSPGGLSLHSAPSSDSDSGGGGLSPEPPEPPPPAAKGPGAHASGAAGTAPAPPGEPPAPGT
CDPAFYPSQRSGAGPQPRPGRPADKDAASCGPGAAVAAVERGAAGLPKASPFSVESLLSDSPPRRKAASN
AAAAAAAGLDFAPGLPCAPRTLIGKGHFLLYPITQPLGFLVPQAALKGGAGLEPAPKDAPPAPAVPPAPP
AQASFGAFSGPGGAPDSAFARRSPDAVASPGAPAPAPAPFRDLASAAATEGGGGDCADAGTAGPAPPPPA
PSPRPGPRPPSPAEEPATCGVPEPGAAAGPSPPEGEELDMD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080461
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080461.3
RefSeq Size 1596 bp
RefSeq ORF 1596 bp
Locus ID 340260
UniProt ID A6NJT0
Cytogenetics 7p22.3
MW 53.5 kDa
Summary This gene encodes a homeobox transcription factor that is involved in somitogenesis and neurogenesis and is required for the maintenance and differentiation of specific elements of the axial skeleton. This gene also plays a role in controlling the development of connections of hypothalamic neurons to pituitary elements, allowing central neurons to reach the peripheral blood circulation and deliver hormones that control peripheral functions. The expression of this gene is associated with an increased frequency of acute myeloid leukemia. provided by RefSeq, Jul 2017
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Documents
Resources
"NM_001080461" in other vectors (6)
SKU Description Size Price
RC222265L1 Lenti ORF clone of Human UNC homeobox (UNCX), Myc-DDK-tagged 10 ug
$794.00
RC222265L2 Lenti ORF clone of Human UNC homeobox (UNCX), mGFP tagged 10 ug
$794.00
RC222265L3 Lenti ORF clone of Human UNC homeobox (UNCX), Myc-DDK-tagged 10 ug
$794.00
RC222265L4 Lenti ORF clone of Human UNC homeobox (UNCX), mGFP tagged 10 ug
$794.00
RG222265 UNCX (tGFP-tagged) - Human UNC homeobox (UNCX) 10 ug
$694.00
SC315791 UNCX (untagged)-Human UNC homeobox (UNCX) 10 ug
$545.00

Plasmid Map for RC222265

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222265
Circular map for RC222265

file-alt Citations

Epigenetically induced ectopic expression of UNCX impairs the proliferation and differentiation of myeloid cells
Daniele, Simonetti, Fusilli et al
Haematologica (2017)
AI Summary: OriGene's Myc-DDK-tagged ORF clone of Homo sapiens UNCX was used as the source of the UNCX gene for constructing the LUNCXI ΔN retroviral vector, enabling the study of UNCX's role in leukemia cell proliferation and differentiation.
Snippet: The UNCX gene was excised from a Myc -DDK- tagged ORF clone of Homo sapiens UNC HB (Origene, Bologna, Italy) by using the Bgl II and Pme I restriction sites and cloned in the same sites of the LXI Δ N vector 12 , which resulted in construction of the LUNCXI Δ N retroviral vector
Showing 1-1 of 1 papers.
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