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OR8A1 (NM_001005194) Human Tagged ORF Clone

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SKU
RC222254
OR8A1 (Myc-DDK-tagged)-Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OR8A1
Synonyms OR11-318; OST025
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222254 representing NM_001005194
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTTCTTGTCTCCCATGCATCCCTGCAGGCCTCCCACCCAGAGGAGAATGGCTGCAGGAAATCACT
CTACAGTGACAGAGTTCATTCTCAAGGGTTTAACGAAGAGAGCAGACCTCCAGCTCCCCCTCTTTCTCCT
CTTCCTCGGGATCTACTTGGTCACCATCGTGGGGAACCTGGGCATGATCACTCTAATTTGTCTGAACTCT
CAGCTGCACACCCCCATGTACTACTTTCTCAGCAATCTGTCACTCATGGATCTCTGCTACTCCTCCGTCA
TTACCCCTAAGATGCTGGTGAACTTTGTGTCAGAGAAAAACATCATCTCCTACGCAGGGTGCATGTCACA
GCTCTACTTCTTCCTTGTTTTTGTCATTGCTGAGTGTTACATGCTGACAGTGATGGCCTACGACCGCTAT
GTTGCCATCTGCCACCCTTTGCTTTACAACATCATTATGTCTCATCACACCTGCCTGCTGCTGGTGGCTG
TGGTCTACGCCATCGGACTCATTGGCTCCACAATAGAAACTGGCCTCATGTTAAAACTGCCCTATTGTGA
GCACCTCATCAGTCACTACTTCTGTGACATCCTCCCTCTCATGAAGCTGTCCTGCTCTAGCACCTATGAT
GTTGAGATGACAGTCTTCTTTTCGGCTGGATTCAACATCATAGTCACGAGCTTAACAGTTCTTGTTTCTT
ACACCTTCATTCTCTCCAGCATCCTCGGCATCAGCACCACAGAGGGGAGATCCAAAGCCTTCAGCACCTG
CAGCTCCCACCTTGCAGCCGTGGGAATGTTCTATGGATCAACTGCATTCATGTACTTAAAACCCTCCACA
ATCAGTTCCTTGACCCAGGAGAATGTGGCCTCTGTGTTCTACACCACGGTAATCCCCATGTTGAATCCCC
TAATCTACAGCCTGAGGAACAAGGAAGTAAAGGCTGCCGTGCAGAAAACGCTGAGGGGTAAACTGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222254 representing NM_001005194
Red=Cloning site Green=Tags(s)

MGFLSPMHPCRPPTQRRMAAGNHSTVTEFILKGLTKRADLQLPLFLLFLGIYLVTIVGNLGMITLICLNS
QLHTPMYYFLSNLSLMDLCYSSVITPKMLVNFVSEKNIISYAGCMSQLYFFLVFVIAECYMLTVMAYDRY
VAICHPLLYNIIMSHHTCLLLVAVVYAIGLIGSTIETGLMLKLPYCEHLISHYFCDILPLMKLSCSSTYD
VEMTVFFSAGFNIIVTSLTVLVSYTFILSSILGISTTEGRSKAFSTCSSHLAAVGMFYGSTAFMYLKPST
ISSLTQENVASVFYTTVIPMLNPLIYSLRNKEVKAAVQKTLRGKLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005194
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005194.1, NP_001005194.1
RefSeq Size 981 bp
RefSeq ORF 930 bp
Locus ID 390275
UniProt ID Q8NGG7
Cytogenetics 11q24.2
Protein Families Transmembrane
Protein Pathways Olfactory transduction
MW 36.2 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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"NM_001005194" in other vectors (6)
SKU Description Size Price
RC222254L1 Lenti ORF clone of Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1), Myc-DDK-tagged 10 ug
$600.00
RC222254L2 Lenti ORF clone of Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1), mGFP tagged 10 ug
$600.00
RC222254L3 Lenti ORF clone of Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1), Myc-DDK-tagged 10 ug
$600.00
RC222254L4 Lenti ORF clone of Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1), mGFP tagged 10 ug
$600.00
RG222254 OR8A1 (tGFP-tagged) - Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1) 10 ug
$489.00 $500.00
SC300834 OR8A1 (untagged)-Human olfactory receptor, family 8, subfamily A, member 1 (OR8A1) 10 ug
$300.00

Plasmid Map for RC222254

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222254
Circular map for RC222254

file-alt Citations

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