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DNAJC14 (NM_032364) Human Tagged ORF Clone

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SKU
RC222220
DNAJC14 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $643.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJC14
Synonyms DNAJ; DRIP78; HDJ3; LIP6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222220 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGAAGCACCCCGGAGAAAGAGGGTTGTATGGAGCCCACCACAGTGGTGGTGCCTCCCTCAGGA
CTTTAGGACCCTCCGTGGACCCTGAAATACCTTCATTCTCAGGACTCAGGGACTCAGCAGGGACTGCTCC
TAATGGTACCCGCTGCCTCACAGAGCACTCTGGTCCTAAGCACACACAGCACCCAAACCCAGCCCATTGG
TTGGACCCAAGCCATGGCCCCCCAGGGGGTCCAGGACCACCTAGAGATGCAGAGGACCCTGATCAGAGTG
AGACGTCTTCAGAAGAAGAATCAGGAGTGGACCAGGAACTCTCAAAAGAAAACGAGACTGGGAACCAGAA
GGATGGGAACTCTTTTCTTTCCATTCCATCTGCTTGCAACTGCCAGGGAACACCTGGAATTCCAGAAGGG
CCTTACTCTGAGGGAGGAAATGGTTCTTCTAGCAACTTTTGCCACCACTGTACCTCTCCAGCTTTGGGGG
AAGATGAGTTGGAAGAGGAATATGATGATGAAGAATCTCTCAAGTTCCCCAGTGATTTTTCGCGTGTGTC
CAGCGGAAAGAAACCCCCATCCCGGAGACAGCGGCACCGCTTTCCAACGAAGGAGGATACTCGGGAGGGT
GGACGTAGGGATCCCAGGTCCCCTGGTCGACATCGGCTGGGTCGGAAACGAAGTCAGGCAGATAAGCGCA
AAGGCCTGGGATTGTGGGGAGCCGAGGAACTATGTCAACTTGGACAGGCAGGCTTTTGGTGGCTGATTGA
ACTGCTGGTATTGGTGGGAGAGTACGTAGAAACTTGTGGCCATCTCATCTATGCCTGCAGGCAACTGAAA
AGCAGTGATTTGGACCTTTTTCGAGTTTGGATGGGAGTGTGGACAGGGCGGTTAGGGGGCTGGGCCCAGG
TCATGTTTCAGTTTCTAAGCCAGGGGTTTTACTGTGGAGTAGGACTGTTTACTCGTTTTCTTAAGCTGCT
GGGTGCTTTGCTGCTCCTGGCTCTGGCCCTCTTTTTGGGCTTTCTACAGTTGGGATGGCGGTTTCTGGTG
GGACTAGGTGACCGGTTAGGCTGGAGGGATAAGGCTACCTGGCTCTTCTCTTGGCTGGATTCTCCAGCCT
TGCAGCGTTGCTTGACTCTGCTGAGAGATAGCAGGCCATGGCAGCGGCTGGTAAGAATAGTTCAGTGGGG
CTGGCTGGAGTTGCCTTGGGTCAAGCAGAATATTAATAGGCAGGGGAATGCACCTGTAGCTAGTGGGCGC
TACTGCCAGCCTGAAGAGGAAGTGGCTCGACTCTTGACCATGGCTGGGGTTCCTGAGGATGAGCTAAACC
CTTTCCATGTACTGGGGGTTGAGGCCACAGCATCAGATGTTGAACTGAAGAAGGCCTATAGACAGCTGGC
AGTGATGGTTCATCCTGACAAAAATCATCATCCCCGGGCTGAGGAGGCCTTCAAGGTTTTGCGAGCAGCT
TGGGACATTGTCAGCAATGCTGAAAAGCGAAAGGAGTATGAGATGAAACGAATGGCAGAGAATGAGCTGA
GCCGGTCAGTAAATGAGTTTCTGTCCAAGCTGCAAGATGACCTCAAGGAGGCAATGAATACTATGATGTG
TAGCCGATGCCAAGGAAAGCATAGGAGGTTTGAAATGGACCGGGAACCTAAGAGTGCCAGATACTGTGCT
GAGTGTAATAGGCTGCATCCTGCTGAGGAAGGAGACTTTTGGGCAGAGTCAAGCATGTTGGGCCTCAAGA
TCACCTACTTTGCACTGATGGATGGAAAGGTGTATGACATCACAGAGTGGGCTGGATGCCAGCGTGTAGG
TATCTCCCCAGATACCCACAGAGTCCCCTATCACATCTCATTTGGTTCTCGGATTCCAGGCACCAGAGGG
CGGCAGAGAGCCACCCCAGATGCCCCTCCTGCTGATCTTCAGGATTTCTTGAGTCGGATCTTTCAAGTAC
CCCCAGGGCAGATGCCCAATGGGAACTTCTTTGCAGCTCCTCAGCCTGCCCCTGGAGCCGCTGCAGCCTC
TAAGCCCAACAGCACAGTACCCAAGGGAGAAGCCAAACCTAAGCGGCGGAAGAAAGTGAGGAGGCCCTTC
CAACGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222220 protein sequence
Red=Cloning site Green=Tags(s)

MAQKHPGERGLYGAHHSGGASLRTLGPSVDPEIPSFSGLRDSAGTAPNGTRCLTEHSGPKHTQHPNPAHW
LDPSHGPPGGPGPPRDAEDPDQSETSSEEESGVDQELSKENETGNQKDGNSFLSIPSACNCQGTPGIPEG
PYSEGGNGSSSNFCHHCTSPALGEDELEEEYDDEESLKFPSDFSRVSSGKKPPSRRQRHRFPTKEDTREG
GRRDPRSPGRHRLGRKRSQADKRKGLGLWGAEELCQLGQAGFWWLIELLVLVGEYVETCGHLIYACRQLK
SSDLDLFRVWMGVWTGRLGGWAQVMFQFLSQGFYCGVGLFTRFLKLLGALLLLALALFLGFLQLGWRFLV
GLGDRLGWRDKATWLFSWLDSPALQRCLTLLRDSRPWQRLVRIVQWGWLELPWVKQNINRQGNAPVASGR
YCQPEEEVARLLTMAGVPEDELNPFHVLGVEATASDVELKKAYRQLAVMVHPDKNHHPRAEEAFKVLRAA
WDIVSNAEKRKEYEMKRMAENELSRSVNEFLSKLQDDLKEAMNTMMCSRCQGKHRRFEMDREPKSARYCA
ECNRLHPAEEGDFWAESSMLGLKITYFALMDGKVYDITEWAGCQRVGISPDTHRVPYHISFGSRIPGTRG
RQRATPDAPPADLQDFLSRIFQVPPGQMPNGNFFAAPQPAPGAAAASKPNSTVPKGEAKPKRRKKVRRPF
QR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032364
ORF Size 2106 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032364.5, NP_115740.5
RefSeq Size 3330 bp
RefSeq ORF 2109 bp
Locus ID 85406
UniProt ID Q6Y2X3, A0A024RB64
Cytogenetics 12q13.2
Domains DnaJ
Protein Families Druggable Genome, Transmembrane
MW 78.6 kDa
Summary Regulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface.UniProtKB/Swiss-Prot Function
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"NM_032364" in other vectors (7)
SKU Description Size Price
RC222220L1 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14), Myc-DDK-tagged 10 ug
$943.00
RC222220L2 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14), mGFP tagged 10 ug
$943.00
RC222220L3 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14), Myc-DDK-tagged 10 ug
$943.00
RC222220L4 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14), mGFP tagged 10 ug
$943.00
RG222220 DNAJC14 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14) 10 ug
$789.00 $843.00
SC104958 DNAJC14 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14) 10 ug
$708.00
SC318182 DNAJC14 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 14 (DNAJC14) 10 ug
$644.00

Plasmid Map for RC222220

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222220
Circular map for RC222220

file-alt Citations

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