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POLR1F (NM_001002926) Human Tagged ORF Clone

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SKU
RC222203
TWISTNB (Myc-DDK-tagged)-Human TWIST neighbor (TWISTNB)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "POLR1F or NM_001002926"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POLR1F
Synonyms A43; RPA43; TWISTNB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222203 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAGGTTGCTCAGAGGCGCCGCGGCCAGCGGCGGCTTCTGATGGGTCTCTGGTAGGGCAGGCTG
GCGTCCTGCCTTGCCTAGAGTTGCCGACTTATGCCGCTGCTTGTGCGCTGGTGAACAGTCGCTACTCATG
CCTGGTGGCCGGGCCGCACCAAAGGCACATCGCGCTGTCGCCCCGCTACCTTAACAGGAAACGCACCGGC
ATTCGAGAACAGCTTGATGCGGAGCTCCTTCGCTATTCTGAGAGCCTTTTAGGTGTCCCTATTGCATATG
ATAACATCAAAGTTGTGGGAGAGCTTGGAGATATTTATGATGATCAAGGACACATTCATCTTAACATTGA
AGCCGATTTTGTTATTTTCTGCCCTGAACCAGGGCAGAAGCTTATGGGTATAGTTAATAAAGTGTCTTCT
AGCCACATTGGCTGTTTAGTACATGGGTGTTTCAATGCCTCCATTCCTAAACCTGAGCAGTTGTCAGCTG
AGCAGTGGCAAACCATGGAGATAAACATGGGTGATGAACTAGAATTTGAAGTATTTCGTTTAGACTCAGA
TGCTGCTGGAGTATTCTGCATTCGGGGAAAACTAAATATCACAAGTTTACAATTCAAGCGCTCTGAAGTT
TCTGAAGAAGTTACAGAAAATGGCACTGAGGAAGCTGCTAAAAAACCTAAAAAGAAGAAAAAGAAGAAAG
ACCCAGAGACATATGAAGTGGACAGTGGTACCACAAAGCTAGCAGATGATGCAGATGACACTCCAATGGA
AGAGTCAGCCCTGCAGAATACTAATAATGCGAATGGCATCTGGGAGGAGGAGCCAAAGAAAAAGAAGAAG
AAGAAAAAGCACCAGGAAGTTCAGGACCAGGACCCTGTTTTCCAAGGCAGTGACTCCAGTGGTTACCAAA
GTGACCATAAAAAGAAAAAAAAGAAAAGAAAACACAGTGAAGAGGCCGAATTTACCCCACCTTTGAAATG
CTCACCAAAAAGAAAAGGGAAAAGTAATTTTCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222203 protein sequence
Red=Cloning site Green=Tags(s)

MAAGCSEAPRPAAASDGSLVGQAGVLPCLELPTYAAACALVNSRYSCLVAGPHQRHIALSPRYLNRKRTG
IREQLDAELLRYSESLLGVPIAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSS
SHIGCLVHGCFNASIPKPEQLSAEQWQTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNITSLQFKRSEV
SEEVTENGTEEAAKKPKKKKKKKDPETYEVDSGTTKLADDADDTPMEESALQNTNNANGIWEEEPKKKKK
KKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAEFTPPLKCSPKRKGKSNFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001002926
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001002926.2
RefSeq Size 3892 bp
RefSeq ORF 1017 bp
Locus ID 221830
UniProt ID Q3B726
Cytogenetics 7p21.1
MW 37.4 kDa
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.UniProtKB/Swiss-Prot Function
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"NM_001002926" in other vectors (6)
SKU Description Size Price
RC222203L1 Lenti ORF clone of Human TWIST neighbor (TWISTNB), Myc-DDK-tagged 10 ug
$757.00
RC222203L2 Lenti ORF clone of Human TWIST neighbor (TWISTNB), mGFP tagged 10 ug
$757.00
RC222203L3 Lenti ORF clone of Human TWIST neighbor (TWISTNB), Myc-DDK-tagged 10 ug
$757.00
RC222203L4 Lenti ORF clone of Human TWIST neighbor (TWISTNB), mGFP tagged 10 ug
$757.00
RG222203 TWISTNB (tGFP-tagged) - Human TWIST neighbor (TWISTNB) 10 ug
$489.00 $657.00
SC300493 TWISTNB (untagged)-Human TWIST neighbor (TWISTNB) 10 ug
$503.00

Plasmid Map for RC222203

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222203
Circular map for RC222203

file-alt Citations

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