Kv1.2 (KCNA2) (NM_004974) Human Tagged ORF Clone
- TrueORF®
SKU
RC222200
KCNA2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1
$686.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Kv1.2 |
| Synonyms | DEE32; EIEE32; HBK5; HK4; HUKIV; KV1.2; MK2; NGK1; RBK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004974 |
| ORF Size | 1497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004974.4 |
| RefSeq Size | 2142 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 3737 |
| UniProt ID | P16389 |
| Cytogenetics | 1p13.3 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 56.5 kDa |
| Summary | Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004974" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC222200L1 | Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC222200L2 | Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC222200L3 | Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC222200L4 | Lenti ORF clone of Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG222200 | KCNA2 (tGFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1 | 10 ug |
$886.00
|
|
| SC303558 | KCNA2 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
KcsA-Kv1.x chimeras with complete ligand binding sites provide improved predictivity for screening selective Kv1.x blockers
Szekér, Bodó, Klima et al
J Biol Chem (2024)
Szekér, Bodó, Klima et al
J Biol Chem (2024)
Cited by 1
AI Summary: OriGene’s pCMV6-AC-GFP plasmids encoding hKv1.1 (RG211000) and hKv1.2 (RC222200) were used to transiently transfect cells, enabling functional studies of Kv1.x channels in the development and validation of improved screening tools for selective Kv1.x blockers.
Snippet: were transiently transfected with plasmids encoding hKv1.1 (hKCNA1 gene) and hKv1.2 (hKCNA2 gene) in pCMV6-AC-GFP plasmid (RG211000 and RC222200, OriGene Technologies, Rockville, MD) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer’s protocol, then cultured under standard conditions. …
Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, α-KTx 2.24 Has a Picomolar Affinity for Kv1.2
Shakeel, Olamendi-Portugal, Naseem et al
Toxins (Basel) (2023) 15 (8) DOI: 10.3390/toxins15080506
Shakeel, Olamendi-Portugal, Naseem et al
Toxins (Basel) (2023) 15 (8) DOI: 10.3390/toxins15080506
Cited by 5
AI Summary: OriGene’s pCMV6-AC-GFP vectors (cat# RG211000 and RC222200) encoding hKv1.1 and hKv1.2 ion channels were used to transiently transfect CHO cells, enabling electrophysiological assays to assess the inhibitory effects of the newly identified scorpion peptides.
Snippet: in. CHO cells were transiently transfected by using Lipofectamine 2000 kit (Invitrogen, Carlsbad, CA, USA), as per the manufacturer’s protocol with vector pCMV6-AC-GFP (cat# RG211000 and RC222200;OriGene Technologies, Rockville, MD, USA) encoding hKv1.1 (hKCNA1 gene) or hKv1.2 (hKCNA2 gene) ion channels. Cells were grown under standard conditions in an incubator at 37 °C and 5% CO2 for 24 h
Cm28, a scorpion toxin having a unique primary structure, inhibits KV1.2 and KV1.3 with high affinity
Naseem, Carcamo-Noriega, Beltrán-Vidal et al
J Gen Physiol (2022) 154 (8) DOI: 10.1085/jgp.202213146
Naseem, Carcamo-Noriega, Beltrán-Vidal et al
J Gen Physiol (2022) 154 (8) DOI: 10.1085/jgp.202213146
Cited by 18
AI Summary: OriGene products, specifically the hKV1.1 (hKCNA1 gene, cat# RG211000) and hKV1.2 (hKCNA2 gene, cat# RC222200) coding vectors in pCMV6-AC-GFP plasmids, were used to express human potassium channels for functional assays investigating the inhibitory effects of the scorpion toxin Cm28.
Snippet: 00 kit (Invitrogen), as per manufacturer’s protocol with the following ion channel coding vectors: hKV1.1 (hKCNA1 gene) and hKV1.2 (hKCNA2 gene) in pCMV6-AC-GFP plasmid (cat# RG211000 and RC222200;OriGene Technologies), hKV1.5 in pEYFP plasmid (a kind gift from A. Felipe, University of Barcelona, Barcelona, Spain), hKCa3.1 (hKCNN4 gene) in pEGFP-C1 vector (a kind gift from H. Wulff, University;(hKCNA2 gene) in pCMV6-AC-GFP plasmid (cat# RG211000 and RC222200;OriGene Technologies), hKV1.5 in pEYFP plasmid (a kind gift from A. …
Structural basis of the potency and selectivity of Urotoxin, a potent Kv1 blocker from scorpion venom
Luna-Ramirez, Csoti, McArthur et al
Biochem. Pharmacol. (2019) 174, 113782 DOI: 10.1016/j.bcp.2019.113782
Luna-Ramirez, Csoti, McArthur et al
Biochem. Pharmacol. (2019) 174, 113782 DOI: 10.1016/j.bcp.2019.113782
Cited by 19
AI Summary: OriGene Technologies supplied the plasmid cDNAs encoding wild type and mutant human Kv1.1, Kv1.2, Kv1.3, and Kv1.5 channels, which were essential for the expression and functional characterization of these ion channels in the study.
Snippet: VIC, Australia) in a humidified incubator at 37°C, 5% CO 2 . Transient transfection (Lipofectamine 2000, Life Technologies, VIC, Australia) of plasmid cDNAs encoding wild type and mutant human isoforms of Kv1.1, Kv1.2, Kv1.3 or Kv1.5 (OriGene Technologies, MD
Showing 1-4 of 4 papers.
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