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FLAD1 (NM_025207) Human Tagged ORF Clone

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SKU
RC222189
FLAD1 (Myc-DDK-tagged)-Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $546.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLAD1
Synonyms FAD1; FADS; LSMFLAD; PP591
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222189 representing NM_025207
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTGGGATTTGGGAACACGTTTATTCCAGAGGCAGGAACAAAGGAGTCGCTTGTCAAGGATCTGGT
TAGAGAAGACTAGGGTCTTCCTCGAAGGAAGCACGCGCACGCCTGCTCTCCCCCATTGTCTTTTCTGGCT
TCTCCAGGTTCCCTCGACCCAGGACCCCCTGTTCCCAGGCTATGGCCCCCAGTGCCCTGTAGACCTGGCA
GGCCCCCCGTGCTTGCGACCCCTATTTGGGGGTCTGGGTGGCTACTGGAGGGCCTTGCAGAGGGGCAGAG
AAGGCAGGACCATGACATCTAGGGCCTCTGAACTTTCTCCGGGGCGCAGCGTGACGGCTGGCATCATCAT
TGTTGGAGATGAGATCCTTAAGGGACACACTCAGGACACCAACACCTTCTTTCTGTGCCGGACACTGCGC
TCCCTAGGGGTCCAGGTTTGCCGAGTCTCAGTTGTACCTGATGAGGTAGCCACCATTGCAGCTGAGGTCA
CTTCTTTCTCCAACCGCTTCACCCATGTCCTCACAGCAGGGGGCATCGGCCCCACTCATGATGATGTGAC
CTTTGAGGCAGTGGCACAGGCCTTTGGAGATGAGCTGAAGCCACACCCCAAGTTGGAAGCAGCCACCAAA
GCCCTAGGAGGGGAAGGCTGGGAGAAGCTATCATTGGTGCCCTCCTCTGCCCGCCTGCATTATGGCACAG
ATCCTTGCACTGGTCAACCTTTCAGATTCCCTCTGGTCTCCGTCCGAAACGTCTACCTCTTCCCAGGCAT
TCCAGAGCTGCTGCGGCGGGTGCTGGAGGGGATGAAGGGACTATTCCAAAACCCAGCTGTTCAGTTCCAC
TCAAAGGAGCTATATGTGGCTGCTGATGAAGCCTCCATCGCCCCCATTCTGGCTGAGGCCCAGGCCCACT
TTGGACGTAGGCTTGGCCTGGGTTCCTACCCTGACTGGGGCAGCAACTACTATCAGGTGAAGCTGACTCT
AGACTCAGAGGAAGAAGGACCCCTGGAGGAATGCTTGGCCTACCTGACTGCCCGTTTGCCCCAGGGATCG
CTGGTCCCCTACATGCCCAACGCTGTGGAGCAGGCCAGTGAGGCTGTATACAAACTCGCTGAATCAGGGT
CTTCTTTGGGGAAAAAGGTGGCAGGTGCCCTACAGACCATTGAGACCTCCCTGGCTCAGTACAGCCTCAC
CCAGCTCTGTGTGGGCTTCAACGGGGGCAAAGACTGCACTGCCCTCCTGCACCTCTTCCATGCAGCTGTG
CAGAGGAAATTACCTGATGTTCCAAACCCCCTCCAGATCCTGTATATCCGCAGCATCTCCCCTTTCCCTG
AGCTGGAACAGTTTCTACAGGACACTATCAAGAGGTATAATCTGCAGATGTTGGAAGCTGAGGGCAGCAT
GAAGCAGGCCCTGGGTGAACTGCAGGCACGGCACCCCCAGCTGGAGGCTGTCCTTATGGGCACCCGCCGG
ACTGACCCCTACTCCTGTAGCCTCTGCCCTTTCAGCCCCACTGACCCAGGCTGGCCCGCATTCATGCGCA
TCAACCCACTGCTGGACTGGACCTACAGAGACATCTGGGATTTTCTGCGTCAGCTGTTTGTCCCATACTG
TATCCTGTATGACCGAGGATACACATCACTGGGGAGTCGGGAGAATACCGTGCGGAACCCGGCCCTGAAG
TGCCTGAGCCCAGGAGGACACCCCACATACCGTCCAGCCTATCTACTGGAGAACGAAGAAGAGGAGCGGA
ACTCCCGCACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222189 representing NM_025207
Red=Cloning site Green=Tags(s)

MGWDLGTRLFQRQEQRSRLSRIWLEKTRVFLEGSTRTPALPHCLFWLLQVPSTQDPLFPGYGPQCPVDLA
GPPCLRPLFGGLGGYWRALQRGREGRTMTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLR
SLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATK
ALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFH
SKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGS
LVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAV
QRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRR
TDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALK
CLSPGGHPTYRPAYLLENEEEERNSRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025207
ORF Size 1761 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025207.5
RefSeq Size 2218 bp
RefSeq ORF 1764 bp
Locus ID 80308
UniProt ID Q8NFF5
Cytogenetics 1q21.3
Domains MoCF_biosynth
Protein Pathways Metabolic pathways, Riboflavin metabolism
MW 65.1 kDa
Summary This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed. provided by RefSeq, Jul 2008
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Documents
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"NM_025207" in other vectors (4)
SKU Description Size Price
RC222189L3 Lenti-ORF clone of FLAD1 (Myc-DDK-tagged)-Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1 10 ug
$846.00
RC222189L4 Lenti-ORF clone of FLAD1 (mGFP-tagged)-Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1 10 ug
$846.00
RG222189 FLAD1 (tGFP-tagged) - Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1 10 ug
$746.00
SC310239 FLAD1 (untagged)-Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1 10 ug
$547.00

Plasmid Map for RC222189

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222189
Circular map for RC222189

file-alt Citations

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