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TECPR2 (NM_014844) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC221908L3
Lenti-ORF clone of TECPR2 (Myc-DDK-tagged)-Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,628.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TECPR2
Synonyms KIAA0329; SPG49
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC221908).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014844
ORF Size 4233 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014844.3
RefSeq Size 8703 bp
RefSeq ORF 4236 bp
Locus ID 9895
UniProt ID O15040
Cytogenetics 14q32.31
Domains TECPR, WD40
MW 153.8 kDa
Summary The protein encoded by this gene is a member of the tectonin beta-propeller repeat-containing (TECPR) family, and contains both TECPR and tryptophan-aspartic acid repeat (WD repeat) domains. This gene has been implicated in autophagy, as reduced expression levels of this gene have been associated with impaired autophagy. Recessive mutations in this gene have been associated with a hereditary form of spastic paraparesis (HSP). HSP is characterized by progressive spasticity and paralysis of the legs. There is also some evidence linking mutations in this gene with birdshot chorioretinopathy (BSCR), which results in inflammation of the choroid and retina. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_014844" in other vectors (4)
SKU Description Size Price
RC221908 TECPR2 (Myc-DDK-tagged)-Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1 10 ug
$1,328.00
RC221908L4 Lenti-ORF clone of TECPR2 (mGFP-tagged)-Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1 10 ug
$1,628.00
RG221908 TECPR2 (tGFP-tagged) - Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1 10 ug
$1,528.00
SC114803 TECPR2 (untagged)-Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1 10 ug
$2,350.00

Plasmid Map for RC221908L3

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RC221908L3
Circular map for RC221908L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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