MID1IP1 (NM_001098791) Human Tagged ORF Clone

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SKU
RC221845
MID1IP1 (Myc-DDK-tagged)-Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MID1IP1
Synonyms G12-like; MIG12; S14R; STRAIT11499; THRSPL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221845 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCAAATCTGCGACACCTACAACCAGAAGCACTCGCTCTTTAACGCCATGAATCGCTTCATTGGCG
CCGTGAACAACATGGACCAGACGGTGATGGTGCCCAGCTTGCTGCGCGACGTGCCCCTGGCTGACCCCGG
GTTAGACAACGATGTTGGCGTGGAGGTAGGCGGCAGTGGCGGCTGCCTGGAGGAGCGCACGCCCCCAGTC
CCCGACTCGGGAAGCGCCAATGGCAGCTTTTTCGCGCCCTCTCGGGACATGTACAGCCACTACGTGCTTC
TCAAGTCCATCCGCAACGACATCGAGTGGGGGGTCCTGCACCAGCCGCCTCCACCGGCTGGGAGCGAGGA
GGGCAGTGCCTGGAAGTCCAAGGACATCCTGGTGGACCTGGGCCACTTGGAGGGTGCGGACGCCGGCGAA
GAAGACCTGGAACAGCAGTTCCACTACCACCTGCGCGGGCTGCACACTGTGCTCTCGAAACTCACGCGCA
AAGCCAACATCCTCACTAACAGATACAAGCAGGAGATCGGCTTCGGCAATTGGGGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221845 protein sequence
Red=Cloning site Green=Tags(s)

MMQICDTYNQKHSLFNAMNRFIGAVNNMDQTVMVPSLLRDVPLADPGLDNDVGVEVGGSGGCLEERTPPV
PDSGSANGSFFAPSRDMYSHYVLLKSIRNDIEWGVLHQPPPPAGSEEGSAWKSKDILVDLGHLEGADAGE
EDLEQQFHYHLRGLHTVLSKLTRKANILTNRYKQEIGFGNWGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001098791
ORF Size 549 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001098791.1, NP_001092261.1
RefSeq Size 1916 bp
RefSeq ORF 552 bp
Locus ID 58526
UniProt ID Q9NPA3
Cytogenetics Xp11.4
MW 20.2 kDa
Summary Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001098791" in other vectors (6)
SKU Description Size Price
RC221845L1 Lenti ORF clone of Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC221845L2 Lenti ORF clone of Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3, mGFP tagged 10 ug
$600.00
RC221845L3 Lenti ORF clone of Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC221845L4 Lenti ORF clone of Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3, mGFP tagged 10 ug
$600.00
RG221845 MID1IP1 (tGFP-tagged) - Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3 10 ug
$500.00
SC316340 MID1IP1 (untagged)-Human MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) (MID1IP1), transcript variant 3 10 ug
$330.00

Plasmid Map for RC221845

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Cloning scheme for RC221845

Circular map for RC221845

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