ZAR1 (NM_175619) Human Tagged ORF Clone

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SKU
RC221731
ZAR1 (Myc-DDK-tagged)-Human zygote arrest 1 (ZAR1)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZAR1
Synonyms Z3CXXC6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221731 representing NM_175619
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCTGGGGGACGAGGTGCTGGACGGTTACGTGTTCCCGGCGTGCCCCCCCTGCTCGTACCGGT
ACCCATACCCCGCGGCCACCAAGGGCAAGGGCGCGGCGGGCGGCAGCTGGCAGCAGCGCGGCAGGGGCTG
CCTTCCCGCCTCCTCCCCCTGCTCGGCGGGCGCGGCCTCGTTGTCCTTCCCGGGCTGCGGGCGGCTGACG
GCCGCCGAGTACTTCGACAGCTACCAGCGGGAGCGGCTCATGGCTCTCCTGGCGCAGGTGGGGCCGGGTC
TCGGGCCGCGCGCCCGCAGGGCCGGCAGCTGCGACGTGGCGGTGCAGGTGAGCCCGCGCATCGACGCCGC
GGTACAGTGCTCGCTGGGGAGGCGCACGCTGCAGCGCCGGGCCCGCGACCCCGAGTCCCCGGCCGGCCCC
GGGGCCGAGGGCACCACGGGTGGCGGCTCTTTCTCCCAGCAGCCATCCCGTCGAGGCCTGGAGCAGGGCA
GCCCCCAGAACGGCGCCCCGCGGCCCATGCGCTTCCCGCGCACCGTCGCCGTGTACTCGCCCCTGGCCTT
GCGCCGTCTCACCGCCTTCCTGGAGGGGCCCGGGCCCGCGGCGGGCGAGCAGAGGTCCGGGGCGTCGGAC
GGAGAGAGGGGGCCGCCGCCCGCGCGGCTTCAAGGCCCAGAGGAGGGGGAGGTGTGGACGAAGAAGGCGC
CCCGGCGGCCGCAGTCCGACGACGACGGCGAGGCCCAGGCCGCAGTCCGAGCGAGCTGGGAGCAGCCGGC
CGACGGTCCCGAGCTGCCGCCGCGAGAGGCCCAGGAGGGCGAGGCGGCTCCGCGGTCGGCGCTAAGGAGC
CCGGGGCAACCTCCGTCGGCGGGGAGGGCCCGAGACGGCGGCGACGGACGGGAGGCGGCCGTCGCGGGAG
AGGGGCCGTCGCCACGGAGCCCGGAGCTGGGCAAGGAGCGGCTGCGCTTCCAGTTCTTAGAGCAGAAATA
TGGCTATTACCACTGCAAGGACTGCAACATCCGCTGGGAGAGTGCTTATGTGTGGTGTGTACAGGGAACT
AACAAGGTTTACTTCAAACAGTTTTGCAGAACTTGTCAGAAGTCTTATAACCCTTACCGAGTGGAGGATA
TCACCTGTCAAAGTTGTAAACAAACGAGATGTTCCTGCCCAGTAAAACTTCGCCACGTGGACCCTAAACG
GCCCCACCGTCAAGATTTGTGCGGTAGATGCAAAGGCAAACGCCTGTCCTGTGACAGCACTTTCAGCTTC
AAATACATCATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221731 representing NM_175619
Red=Cloning site Green=Tags(s)

MAALGDEVLDGYVFPACPPCSYRYPYPAATKGKGAAGGSWQQRGRGCLPASSPCSAGAASLSFPGCGRLT
AAEYFDSYQRERLMALLAQVGPGLGPRARRAGSCDVAVQVSPRIDAAVQCSLGRRTLQRRARDPESPAGP
GAEGTTGGGSFSQQPSRRGLEQGSPQNGAPRPMRFPRTVAVYSPLALRRLTAFLEGPGPAAGEQRSGASD
GERGPPPARLQGPEEGEVWTKKAPRRPQSDDDGEAQAAVRASWEQPADGPELPPREAQEGEAAPRSALRS
PGQPPSAGRARDGGDGREAAVAGEGPSPRSPELGKERLRFQFLEQKYGYYHCKDCNIRWESAYVWCVQGT
NKVYFKQFCRTCQKSYNPYRVEDITCQSCKQTRCSCPVKLRHVDPKRPHRQDLCGRCKGKRLSCDSTFSF
KYII

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175619
ORF Size 1272 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175619.3
RefSeq Size 1438 bp
RefSeq ORF 1275 bp
Locus ID 326340
UniProt ID Q86SH2, B9EG67
Cytogenetics 4p11
MW 45.7 kDa
Summary This maternal effect gene is oocyte-specific and encodes a protein that is thought to function in the initiation of embryogenesis. A similar protein in mouse is required for female fertility. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_175619" in other vectors (6)
SKU Description Size Price
RC221731L1 Lenti ORF clone of Human zygote arrest 1 (ZAR1), Myc-DDK-tagged 10 ug
$986.00
RC221731L2 Lenti ORF clone of Human zygote arrest 1 (ZAR1), mGFP tagged 10 ug
$986.00
RC221731L3 Lenti ORF clone of Human zygote arrest 1 (ZAR1), Myc-DDK-tagged 10 ug
$986.00
RC221731L4 Lenti ORF clone of Human zygote arrest 1 (ZAR1), mGFP tagged 10 ug
$986.00
RG221731 ZAR1 (tGFP-tagged) - Human zygote arrest 1 (ZAR1) 10 ug
$886.00
SC306999 ZAR1 (untagged)-Human zygote arrest 1 (ZAR1) 10 ug
$686.00

Plasmid Map for RC221731

Download sequence for

Cloning scheme for RC221731

Circular map for RC221731

file-alt Citations

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