TOR1AIP1 (NM_015602) Human Tagged ORF Clone
$543.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TOR1AIP1 |
| Synonyms | LAP1; LAP1B; LAP1C; LGMD2Y |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_015602 |
| ORF Size | 1749 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015602.4 |
| RefSeq Size | 3824 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 26092 |
| UniProt ID | Q5JTV8 |
| Cytogenetics | 1q25.2 |
| MW | 66.1 kDa |
| Summary | This gene encodes a type 2 integral membrane protein that binds A- and B-type lamins. The encoded protein localizes to the inner nuclear membrane and may be involved in maintaining the attachment of the nuclear membrane to the nuclear lamina during cell division. Alternate splicing results in multiple transcript variants. provided by RefSeq, Apr 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015602" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221686L3 | Lenti ORF clone of Human torsin A interacting protein 1 (TOR1AIP1), Myc-DDK-tagged | 10 ug |
$843.00
|
|
| RC221686L4 | Lenti ORF clone of Human torsin A interacting protein 1 (TOR1AIP1), mGFP tagged | 10 ug |
$843.00
|
|
| RG221686 | TOR1AIP1 (tGFP-tagged) - Human torsin A interacting protein 1 (TOR1AIP1) | 10 ug |
$743.00
|
|
| SC314100 | TOR1AIP1 (untagged)-Human torsin A interacting protein 1 (TOR1AIP1) | 10 ug |
$544.00
|
|
Citations
Two novel cases further expand the phenotype of TOR1AIP1-associated nuclear envelopathies
Lessel, Chen, Lüttgen et al
Hum. Genet. (2020) 139 (4), 483-498 DOI: 10.1007/s00439-019-02105-6
Lessel, Chen, Lüttgen et al
Hum. Genet. (2020) 139 (4), 483-498 DOI: 10.1007/s00439-019-02105-6
Cited by 17
AI Summary: OriGene’s Myc-Flag-tagged TOR1AIP1 plasmid (RC221686) was used for ectopic expression experiments to assess the functional effects of TOR1AIP1 variants in patient-derived cells.
Snippet: Plasmid-encoding Myc-Flag-tagged TOR1AIP1 (Origene, RC221686) was used in all experiments. 70.000 cells were seeded on 6-well cell culture plates for 24 h to ensure ~ 70% confluent cultures 24 h after seeding. 4 µg of plasmid DNA was transfected for additional 24 h using the Viromer Red reagent according to manufacturer’s instructions (Lipocalyx, Halle, Germany)
Showing 1-1 of 1 papers.
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