Fibulin 1 (FBLN1) (NM_006486) Human Tagged ORF Clone
$644.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Fibulin 1 |
| Synonyms | FBLN; FIBL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006486 |
| ORF Size | 2109 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006486.3 |
| RefSeq Size | 2947 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 2192 |
| UniProt ID | P23142, Q8NBH6 |
| Cytogenetics | 22q13.31 |
| Domains | ANATO, EGF, EGF_CA |
| Protein Families | Secreted Protein |
| MW | 77.24 kDa |
| Summary | Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006486" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221597L3 | Lenti ORF clone of Human fibulin 1 (FBLN1), transcript variant D, Myc-DDK-tagged | 10 ug |
$944.00
|
|
| RC221597L4 | Lenti ORF clone of Human fibulin 1 (FBLN1), transcript variant D, mGFP tagged | 10 ug |
$944.00
|
|
| RG221597 | FBLN1 (tGFP-tagged) - Human fibulin 1 (FBLN1), transcript variant D | 10 ug |
$844.00
|
|
| SC116062 | FBLN1 (untagged)-Human fibulin 1 (FBLN1), transcript variant D | 10 ug |
$645.00
|
|
Citations
The molecular interaction of ADAMTS-1 and fibulin-1 and its potential contribution to breast cancer biology
JCMT (2019) 2019 DOI: 10.20517/2394-4722.2018.81
JCMT (2019) 2019 DOI: 10.20517/2394-4722.2018.81
AI Summary: OriGene’s Flag-tagged ADAMTS-1 cDNA vector was used for transfection experiments to study the effects of ADAMTS-1 overexpression on cell migration in breast cancer cell lines.
Snippet: For transfection experiments, vectors containing the full-length human cDNAs for fibulin-1 (kindly provided by Dr. Tatako Sasaki, Oita University, Japan) and Flag-tagged ADAMTS-1 (Origene) were transfected into cells at 75% confluence using TransIT-X2 (Mirus) Cached
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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