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ZSWIM5 (NM_020883) Human Tagged ORF Clone

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SKU
RC221559
ZSWIM5 (Myc-DDK-tagged)-Human zinc finger, SWIM-type containing 5 (ZSWIM5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,031.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZSWIM5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221559 representing NM_020883
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGGAGGTGAGCGAGAGGAGCTGCTCTCGCCGTCACCGGTCTCTCCGGCTAAGCGGCAGTGTT
CGTGGCCCAGCCCGCAGGCTCATCACCCTCGCGGTTCGCCTGGGGCAGCCGGCGGAGGGGCAGGGGGCGT
CGGCAGCAGCTGCCTGGTCCTCGGGGCCCGCCCCCACCTGCAGCCGGATTCCTTACTGGACTGCGCCGCC
AAGACGGTGGCGGAAAAGTGGGCATACGAACGGGTGGAGGAGCGCTTCGAGCGGATCCCGGAGCCCGTGC
AGCGCCGCATCGTCTACTGGTCCTTCCCGCGGAATGAGCGGGAGATCTGCATGTACTCCAGCTTCCAGTA
CCGGGGCGGCCCCGGCGCCGGCGCTGCTGGCGGCGCCGCGGGGGCTTCGCCGGCCGAGGAGGGGCCGCAG
CCACCCCCCGGGGCCGCCGCTCCGGCCGGCTCCGCCCCCGGGGGCGTCGCGGCTGGGGCATCCCCCGGGC
TGGGCGCAGGGGCCGGCGCGGCCGGCTGCGGTGGCGAGGGGCTCCCGTTCCGCCGGGGCATCCGTCTGCT
GGACAGCGGCTCCGTGGAGAACGTGCTGCAAGTCGGCTTTCATCTGAGTGGCACAGTAACTGAGCTGGCC
ACTGCCTCTGAACCAGCAGTGACTTATAAAGTTGCAATCAGTTTTGATCGATGCAAAATCACCTCAGTGA
CATGTGGCTGTGGGAACAAGGACATATTCTACTGTGCCCATGTGGTAGCACTCTCACTCTACAGGATCCG
TAAACCAGACCAAGTCAAATTGCGTCTTCCTATCTCCGAAACCCTTTTCCAGATGAATAGGGACCAGCTA
CAAAAGTTTATTCAATATTTAATCACGGCACACCACACTGAAGTTCTTCCTACTGCACAGAAACTGGCAG
ATGAGATTCTATCCTCCAACTCAGAAATCAACCAAGTGAATGGTGCCCCTGACCCCACAGCTGGGGCCAG
CATTGACGATGAGAACTGTTGGCATTTGGATGAAGAACAGGTGAAAGAACAAGTGAAGCTTTTTCTCTCC
CAGGGCGGTTACTGTGGCTCTGGAAAGCAACTCAACTCAATGTTTGCCAAGGTTCGAGAAATGCTGCGGA
TGAGAGATTCCAATGGAGCAAGGATGCTGACACTAATAACTGAGCAGTTTGTGGCTGACCCACGACTCAC
ACTCTGGAGGCAGCAAGGAACAAACATGACAGACAAGTGCAGGCAGCTCTGGGATGAGCTAGGGGCTTTA
TGGGTGTGCATAATTTTAAATCCACACTGCAAACTGGAGGAGAAATCCTGCTGGCTGCAGCAGCTGCAGA
AGTGGAGCGACCTGGATGTCTGTCCCCTGGAGGATGGAAACTATGGACATGAGCTGCCCAACATCACCAA
TGCACTTCCCCAGAGTGCCATTCACAGCCCAGACTCCTTATCCAGACCAAGACGAACAGTATTCACTAGA
GCCATTGAGGGGCGTGAATTACATTGGCAGGATAGCCACCTACAGCGAATAATCAGCAGTGATGTTTATA
CAGCTCCTGCCTGCCAGCGGGAAAGTGAAAGACTACTCTTTAATTCCCAAGGCCAGCCACTGTGGCTTGA
GCATGTGCCTACAGCCTGTGCTCGAGTTGACGCCCTGCGTTCCCATGGTTATCCAAAAGAGGCCCTCAGA
CTCACAGTGGCCATTATTAATACTCTGAGGTTGCAGCAGCAGCGGCAACTGGAAATCTACAAGCATCAGA
AGAAAGAACTGTTGCAGAGAGGAACCACAACCATCACAAACCTGGAGGGCTGGGTGGGCCACCCCCTGGA
TCCCATCGACTGCCTGTTTCTCACTTTGACTGAGGCCTGCCGCCTGAATGATGATGGCTATCTGGAGATG
TCAGATATGAATGAAAGCAGACCCCCTGTGTACCAGCATGTACCTGTGGCTGCAGGCTCCCCAAACAGCA
GTGAGTCCTACCTGTCATTGGCCCTGGAAGTTGCACTGATGGGCCTGGGTCAACAGAGGTTAATGCCTGA
AGGCCTCTACGCACAGGACAAGGTATGCCGTAATGAGGAGCAGCTCCTGAGCCAACTCCAAGAGCTGCAG
CTGGACGATGAGCTAGTGCAAACACTGCAAAAACAATGCATCTTACTGCTGGAAGGAGGTCCTTTCAGCG
GTCTGGGAGAAGTCATCCACCGAGAGAGTGTTCCCATGCATACCTTTGCCAAGTATTTGTTCTCAGCTCT
GCTGCCTCATGATCCAGACCTGTCCTATAAATTAGCCTTACGTGCCATGAGGTTACCTGTTTTAGAAAAC
TCAGCTTCTGCAGGCGACACATCCCACCCTCACCATATGGTGTCTGTGGTGCCCAGCCGTTATCCTCGCT
GGTTCACGCTTGGACACTTGGAATCACAGCAGTGTGAACTGGCCTCCACCATGCTGACTGCTGCCAAAGG
AGACACTTTGAGACTCCGAACAATTCTGGAAGCAATACAGAAGCACATTCATTCTTCCTCCCTCATCTTC
AAACTGGCCCAAGATGCATTCAAGATTGCTACTCCCACCGACAGTAGTACTGACAGCACCCTGCTCAACG
TTGCCCTGGAACTTGGGTTACAGGTGATGCGGATGACCTTATCAACCCTTAACTGGAGACGCAGGGAGAT
GGTACGATGGTTGGTGACCTGTGCTACAGAAGTGGGTGTGCGGGCCCTGGTGAGCATCTTGCAGAGCTGG
TACACACTCTTCACCCCTACTGAGGCTACTAGTATTGTAGCTGCCACAGCCGTATCCCACACCACTATCC
TGCGCCTCAGTCTTGACTATCCACAGCGGGAGGAACTGGCTAGCTGTGCTCGCACACTGGCTCTACAGTG
TGCTATGAAGGATCCACAGAGCTGTGCCCTGTCAGCCCTTACACTCTGTGAGAAAGACCACATTGCCTTT
GAGGCAGCCTACCAGATTGCCATTGATGCTGCTGCTGGTGGTATGACCCATTCACAGCTGTTCACCATCG
CCCGCTACATGGAGCTCCGTGGCTACCCGCTACGTGCCTTCAAGCTGGCCTCATTGGCCATGAGCCATCT
TAACCTGGCCTACAACCAGGATACTCACCCAGCCATCAATGATGTGCTCTGGGCTTGTGCTCTCAGCCAC
TCTCTGGGCAAGAATGAGCTGGCAGCTCTCATCCCCCTGGTGGTGAAGAGTGTGCACTGTGCCACAGTGC
TTTCAGACATCTTACGACGCTGCACAGTGACTGCACCTGGCCTGGCCGGCATCCCAGGCCGCCGAAGCTC
TGGAAAGCTCATGTCCACTGACAAAGCTCCATTGCGCCAGTTGCTGGATGCCACCATCAATGCCTATATC
AACACTACACACTCACGCCTAACACACATCAGCCCTCGCCACTATGGAGAGTTCATTGAGTTTCTAAGCA
AGGCTCGGGAGACCTTCCTGCTGCCCCAGGATGGCCACCTGCAGTTTGCCCAGTTCATCGACAACCTCAA
ACAGATCTACAAAGGCAAGAAAAAGTTGATGCTGCTGGTGCGAGAGCGCTTTGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221559 representing NM_020883
Red=Cloning site Green=Tags(s)

MADGGEREELLSPSPVSPAKRQCSWPSPQAHHPRGSPGAAGGGAGGVGSSCLVLGARPHLQPDSLLDCAA
KTVAEKWAYERVEERFERIPEPVQRRIVYWSFPRNEREICMYSSFQYRGGPGAGAAGGAAGASPAEEGPQ
PPPGAAAPAGSAPGGVAAGASPGLGAGAGAAGCGGEGLPFRRGIRLLDSGSVENVLQVGFHLSGTVTELA
TASEPAVTYKVAISFDRCKITSVTCGCGNKDIFYCAHVVALSLYRIRKPDQVKLRLPISETLFQMNRDQL
QKFIQYLITAHHTEVLPTAQKLADEILSSNSEINQVNGAPDPTAGASIDDENCWHLDEEQVKEQVKLFLS
QGGYCGSGKQLNSMFAKVREMLRMRDSNGARMLTLITEQFVADPRLTLWRQQGTNMTDKCRQLWDELGAL
WVCIILNPHCKLEEKSCWLQQLQKWSDLDVCPLEDGNYGHELPNITNALPQSAIHSPDSLSRPRRTVFTR
AIEGRELHWQDSHLQRIISSDVYTAPACQRESERLLFNSQGQPLWLEHVPTACARVDALRSHGYPKEALR
LTVAIINTLRLQQQRQLEIYKHQKKELLQRGTTTITNLEGWVGHPLDPIDCLFLTLTEACRLNDDGYLEM
SDMNESRPPVYQHVPVAAGSPNSSESYLSLALEVALMGLGQQRLMPEGLYAQDKVCRNEEQLLSQLQELQ
LDDELVQTLQKQCILLLEGGPFSGLGEVIHRESVPMHTFAKYLFSALLPHDPDLSYKLALRAMRLPVLEN
SASAGDTSHPHHMVSVVPSRYPRWFTLGHLESQQCELASTMLTAAKGDTLRLRTILEAIQKHIHSSSLIF
KLAQDAFKIATPTDSSTDSTLLNVALELGLQVMRMTLSTLNWRRREMVRWLVTCATEVGVRALVSILQSW
YTLFTPTEATSIVAATAVSHTTILRLSLDYPQREELASCARTLALQCAMKDPQSCALSALTLCEKDHIAF
EAAYQIAIDAAAGGMTHSQLFTIARYMELRGYPLRAFKLASLAMSHLNLAYNQDTHPAINDVLWACALSH
SLGKNELAALIPLVVKSVHCATVLSDILRRCTVTAPGLAGIPGRRSSGKLMSTDKAPLRQLLDATINAYI
NTTHSRLTHISPRHYGEFIEFLSKARETFLLPQDGHLQFAQFIDNLKQIYKGKKKLMLLVRERFG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020883
ORF Size 3555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020883.2
RefSeq Size 5860 bp
RefSeq ORF 3558 bp
Locus ID 57643
UniProt ID Q9P217
Cytogenetics 1p34.1
MW 130.5 kDa
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Documents
Resources
"NM_020883" in other vectors (4)
SKU Description Size Price
RC221559L3 Lenti-ORF clone of ZSWIM5 (Myc-DDK-tagged)-Human zinc finger, SWIM-type containing 5 (ZSWIM5) 10 ug
$1,331.00
RC221559L4 Lenti-ORF clone of ZSWIM5 (mGFP-tagged)-Human zinc finger, SWIM-type containing 5 (ZSWIM5) 10 ug
$1,331.00
RG221559 ZSWIM5 (tGFP-tagged) - Human zinc finger, SWIM-type containing 5 (ZSWIM5) 10 ug
$1,231.00
SC316588 ZSWIM5 (untagged)-Human zinc finger, SWIM-type containing 5 (ZSWIM5) 10 ug
$1,136.00

Plasmid Map for RC221559

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221559
Circular map for RC221559

file-alt Citations

A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer
Xu, Liu, Ma et al
Cancer Manag Res (2018) 10, 3245-3254 DOI: 10.2147/CMAR.S174355
AI Summary: OriGene's ZSWIM5 plasmid (RC221559) and empty vector were used in this study to investigate the effects of ZSWIM5 expression on the proliferation, invasion, and migration of non-small-cell lung cancer cells.
Snippet: The plasmid vector containing ZSWIM5 (RC221559) and the empty vector were manufactured by OriGene Technologies, Inc. (Rockville, MD, USA). The cells were treated with either siRNAs or plasmids using Lipofectamine 3,000 (Thermo Fisher Scientific) for 48 hours
FigureFigureFigure
Showing 1-1 of 1 papers.
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