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SLC9A2 (NM_003048) Human Tagged ORF Clone

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SKU
RC221550
SLC9A2 (Myc-DDK-tagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $743.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC9A2
Synonyms NHE2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221550 representing NM_003048
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCACTGGGCAACTGGAGGAGCCTGCGGGCGCCACTGCCTCCGATGCTGTTGCTGCTGCTCCTGC
AGGTGGCGGGGCCCGTGGGCGCCCTGGCGGAGACCTTGCTGAACGCGCCGAGGGCCATGGGCACCAGTTC
CAGCCCGCCTAGCCCTGCGAGCGTGGTGGCTCCCGGAACGACGCTGTTCGAGGAGAGCCGGCTGCCTGTG
TTTACGCTGGATTACCCCCACGTGCAGATCCCCTTCGAGATCACCCTTTGGATCCTGCTGGCCTCCCTGG
CCAAGATTGGCTTCCATCTGTATCACAAGTTGCCCACAATAGTGCCTGAGAGCTGCCTTCTTATAATGGT
TGGACTTCTACTAGGTGGGATTATTTTTGGTGTTGATGAGAAGTCTCCCCCTGCAATGAAGACTGATGTA
TTTTTCTTGTACCTCCTCCCACCCATCGTGCTGGATGCCGGCTATTTCATGCCCACTCGCCCATTCTTTG
AGAACATTGGCACGATTTTCTGGTATGCTGTGGTAGGGACACTTTGGAATTCCATTGGCATTGGGGTGTC
TTTGTTTGGTATCTGCCAGATCGAAGCATTCGGCCTCAGCGACATCACTTTGCTCCAGAACCTGCTCTTT
GGCAGCTTAATCTCAGCTGTCGATCCTGTGGCTGTGCTTGCTGTCTTTGAGAACATTCACGTCAATGAGC
AGCTCTACATCCTGGTCTTTGGAGAGTCCCTGCTGAATGATGCAGTAACAGTGGTCCTGTACAACTTGTT
CAAGTCGTTTTGCCAGATGAAAACCATTGAGACCATTGATGTGTTTGCAGGAATCGCCAACTTCTTTGTT
GTGGGAATCGGTGGGGTGCTGATTGGCATCTTCTTGGGCTTTATAGCGGCATTTACTACTCGATTCACCC
ATAATATCCGAGTGATCGAGCCACTGTTTGTTTTCCTGTACAGTTATTTGTCCTACATCACAGCTGAAAT
GTTTCACCTCTCAGGCATCATGGCAATCACTGCTTGTGCAATGACTATGAATAAGTACGTAGAAGAAAAT
GTATCTCAGAAATCCTACACGACCATCAAGTACTTCATGAAGATGCTGAGCAGTGTCAGCGAAACCTTGA
TCTTCATCTTCATGGGTGTGTCTACCGTGGGCAAGAACCACGAGTGGAACTGGGCCTTCGTCTGCTTCAC
CCTGGCCTTCTGCCTCATGTGGCGAGCCCTGGGTGTTTTTGTCCTGACTCAGGTCATTAATAGGTTCCGG
ACCATTCCCCTGACCTTTAAGGACCAGTTCATCATTGCCTATGGAGGACTTCGAGGTGCCATCTGTTTTG
CGTTAGTGTTTCTCCTTCCTGCTGCTGTGTTTCCTCGGAAAAAATTGTTTATTACGGCTGCCATTGTTGT
CATATTCTTTACTGTCTTCATTCTGGGAATAACTATTCGACCACTGGTGGAGTTTCTTGATGTCAAGAGG
TCCAATAAGAAACAACAAGCTGTCAGTGAAGAAATCTATTGTCGGTTGTTTGATCATGTGAAGACTGGAA
TTGAAGATGTTTGTGGACATTGGGGTCACAACTTTTGGAGAGACAAGTTTAAGAAGTTTGATGATAAATA
TCTGCGGAAGCTTTTGATTCGGGAAAACCAACCAAAGTCAAGTATTGTATCTTTATATAAAAAGCTTGAA
ATAAAACATGCCATTGAGATGGCAGAGACTGGGATGATAAGTACTGTCCCTACATTTGCATCTCTAAATG
ATTGTCGTGAAGAAAAAATAAGGAAGGTCACGTCCAGTGAAACTGATGAAATTCGAGAACTCTTATCAAG
AAATCTCTATCAAATCCGTCAGCGAACTTTATCCTACAACAGACACAGTCTGACAGCCGACACAAGTGAG
AGACAAGCCAAGGAGATTCTGATTCGCCGGCGACACAGTTTGCGAGAAAGCATTAGGAAGGACAGCAGCT
TGAATCGAGAACACAGGGCTTCCACTTCAACCTCCCGATATTTATCCTTACCTAAAAATACGAAGCTTCC
AGAAAAGCTACAAAAGAGGAGGACTATTTCTATTGCAGATGGCAATAGCAGCGACTCAGACGCAGATGCC
GGGACCACCGTGCTCAATTTGCAGCCCAGAGCCAGGCGCTTCTTGCCAGAACAGTTCTCCAAGAAATCCC
CCCAGTCCTATAAAATGGAATGGAAGAATGAGGTAGATGTTGATTCTGGCCGAGATATGCCCAGCACCCC
CCCAACACCCCACAGCAGAGAAAAGGGCACCCAGACGTCAGGCTTACTACAGCAGCCCCTTCTCTCTAAA
GACCAGTCTGGCTCAGAGAGGGAAGACAGTTTGACTGAAGGCATCCCGCCCAAGCCGCCACCACGGCTGG
TCTGGAGGGCATCGGAACCTGGAAGCCGGAAAGCCCGATTTGGGAGTGAGAAGCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221550 representing NM_003048
Red=Cloning site Green=Tags(s)

MEPLGNWRSLRAPLPPMLLLLLLQVAGPVGALAETLLNAPRAMGTSSSPPSPASVVAPGTTLFEESRLPV
FTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDV
FFLYLLPPIVLDAGYFMPTRPFFENIGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLLF
GSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIETIDVFAGIANFFV
VGIGGVLIGIFLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEEN
VSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLMWRALGVFVLTQVINRFR
TIPLTFKDQFIIAYGGLRGAICFALVFLLPAAVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKR
SNKKQQAVSEEIYCRLFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLE
IKHAIEMAETGMISTVPTFASLNDCREEKIRKVTSSETDEIRELLSRNLYQIRQRTLSYNRHSLTADTSE
RQAKEILIRRRHSLRESIRKDSSLNREHRASTSTSRYLSLPKNTKLPEKLQKRRTISIADGNSSDSDADA
GTTVLNLQPRARRFLPEQFSKKSPQSYKMEWKNEVDVDSGRDMPSTPPTPHSREKGTQTSGLLQQPLLSK
DQSGSEREDSLTEGIPPKPPPRLVWRASEPGSRKARFGSEKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003048
ORF Size 2436 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003048.6
RefSeq Size 5446 bp
RefSeq ORF 2439 bp
Locus ID 6549
UniProt ID Q9UBY0
Cytogenetics 2q12.1
Domains Na_H_Exchanger
Protein Families Druggable Genome, Transmembrane
MW 91.3 kDa
Summary This gene encodes a member of the sodium-hydrogen exchanger (NHE) protein family. These proteins are involved in sodium-ion transport by exchanging intracellular hydrogen ions to external sodium ions and help in the regulation of cell pH and volume. The encoded protein is localized to the apical membrane and is involved in apical absorption of sodium. provided by RefSeq, Jun 2016
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"NM_003048" in other vectors (6)
SKU Description Size Price
RC221550L1 Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2), Myc-DDK-tagged 10 ug
$1,043.00
RC221550L2 Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2), mGFP tagged 10 ug
$1,043.00
RC221550L3 Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2), Myc-DDK-tagged 10 ug
$1,043.00
RC221550L4 Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2), mGFP tagged 10 ug
$1,043.00
RG221550 SLC9A2 (tGFP-tagged) - Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2) 10 ug
$789.00 $943.00
SC118230 SLC9A2 (untagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 2 (SLC9A2) 10 ug
$744.00

Plasmid Map for RC221550

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221550
Circular map for RC221550

file-alt Citations

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