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ZBTB8OS (NM_178547) Human Tagged ORF Clone

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SKU
RC221460
ZBTB8OS (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "ZBTB8OS or NM_178547"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZBTB8OS
Synonyms ARCH; ARCH2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221460 representing NM_178547
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGCGGAAGTAGAGTGTCTAATCCTGCAGTCATGGCGCAGGAAGAGGAAGATGTTAGAGATTACA
ATTTGACTGAAGAACAGAAGGCGATCAAGGCCAAGTATCCGCCAGTCAATAGGAAGTACGAGTATTTGGA
TCATACAGCAGATGTCCAGTTACACGCATGGGGAGATACTCTGGAGGAAGCATTTGAGCAATGTGCAATG
GCCATGTTTGGTTACATGACAGATACTGGGACAGTGGAGCCCCTCCAAACAGTAGAAGTAGAAACCCAAG
GAGATGACTTACAGTCTCTTCTGTTTCACTTTTTGGATGAATGGCTTTATAAGTTCAGTGCTGATGAATT
CTTCATACCCCGGGAAGTGAAAGTACTTAGCATTGATCAAAGAAATTTCAAATTACGATCAATTGGGTGG
GGAGAAGAATTTTCATTGTCCAAGCACCCTCAGGGAACAGAAGTCAAAGCAATAACATATTCAGCAATGC
AGGTCTATAATGAAGAGAACCCGGAAGTTTTTGTGATCATTGACATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221460 representing NM_178547
Red=Cloning site Green=Tags(s)

MKGGSRVSNPAVMAQEEEDVRDYNLTEEQKAIKAKYPPVNRKYEYLDHTADVQLHAWGDTLEEAFEQCAM
AMFGYMTDTGTVEPLQTVEVETQGDDLQSLLFHFLDEWLYKFSADEFFIPREVKVLSIDQRNFKLRSIGW
GEEFSLSKHPQGTEVKAITYSAMQVYNEENPEVFVIIDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178547
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178547.4
RefSeq Size 717 bp
RefSeq ORF 540 bp
Locus ID 339487
UniProt ID Q8IWT0, A8K0B5
Cytogenetics 1p35.1
MW 20.5 kDa
Summary Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB. Together with DDX1, acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_178547" in other vectors (6)
SKU Description Size Price
RC221460L1 Lenti ORF clone of Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS), Myc-DDK-tagged 10 ug
$600.00
RC221460L2 Lenti ORF clone of Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS), mGFP tagged 10 ug
$600.00
RC221460L3 Lenti ORF clone of Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS), Myc-DDK-tagged 10 ug
$600.00
RC221460L4 Lenti ORF clone of Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS), mGFP tagged 10 ug
$600.00
RG221460 ZBTB8OS (tGFP-tagged) - Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS) 10 ug
$500.00
SC307209 ZBTB8OS (untagged)-Human zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS) 10 ug
$330.00

Plasmid Map for RC221460

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221460
Circular map for RC221460

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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