CD11a (ITGAL) (NM_002209) Human Tagged ORF Clone
SKU
RC221297
ITGAL (Myc-DDK-tagged)-Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide) (ITGAL), transcript variant 1
$1,018.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CD11a |
| Synonyms | CD11A; LFA-1; LFA1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002209 |
| ORF Size | 3510 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002209.3 |
| RefSeq Size | 5133 bp |
| RefSeq ORF | 3513 bp |
| Locus ID | 3683 |
| UniProt ID | P20701, B2RAL6 |
| Cytogenetics | 16p11.2 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis |
| MW | 128.77 kDa |
| Summary | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002209" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221297L1 | Lenti ORF clone of Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,318.00
|
|
| RC221297L2 | Lenti ORF clone of Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1, mGFP tagged | 10 ug |
$1,318.00
|
|
| RC221297L3 | Lenti ORF clone of Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,318.00
|
|
| RC221297L4 | Lenti ORF clone of Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1, mGFP tagged | 10 ug |
$1,318.00
|
|
| RG221297 | ITGAL (tGFP-tagged) - Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1 | 10 ug |
$789.00
$1,218.00
|
|
| SC124031 | ITGAL (untagged)-Human integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide) (ITGAL), transcript variant 1 | 10 ug |
$1,019.00
|
|
Citations
Disruption of tumour-host communication by downregulation of LFA-1 reduces COX-2 and e-NOS expression and inhibits brain metastasis growth
Soto, O'Brien, Andreou et al
Oncotarget (2016)
Soto, O'Brien, Andreou et al
Oncotarget (2016)
Cited by 28
AI Summary: OriGene's pRFP-C-RS shRNA expression vectors targeting the ITGAL (CD11a) gene were used to silence LFA-1 expression in tumour cells, enabling the study of its role in brain metastasis growth and tumour-host communication.
Snippet: Four different shRNA expression vectors were cloned in a pRFP-C-RS plasmid under the U6 promoter for mammalian cell expression specifically designed to bind ITGAL (CD11a) gene (Origene, USA)
Showing 1-1 of 1 papers.
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