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ARHGEF1 (NM_199002) Human Tagged ORF Clone

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SKU
RC221180
ARHGEF1 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $849.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "ARHGEF1 or NM_199002"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARHGEF1
Synonyms GEF1; IMD62; LBCL2; LSC; P115-RHOGEF; SUB1.5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221180 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCTCTTTCCACCTGGAGCAGCCCTGCAGAGCCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGG
CCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATTTTGA
GAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGC
CCAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGCTTTGCTGTC
TGCATGCCGACATGCTGGGCTCACTGGGCCCCAAGGAGGCCAAGAAGGCCTTCCTGGACTTCTACCACAG
CTTCCTGGAGAAGACAGCGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACT
AGGGCTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAG
CCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCT
GGCCCAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAG
CGGCTGCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGG
TCAACGCCATTGGCCTGTACATGCGCCACCTTGGGGTGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAG
GAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAGACCAAGAAGGGGCTG
AGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAG
CAGAGGTTGATGCCGAGAAGCCAGGTGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCG
GAATATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCCCTGGACAGCCCAGAC
CGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGT
CCTTGGCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGA
GCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGGCTGGCGGGAACTCGTCCCCCCAGAC
ACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGG
CGGCCCACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTT
CCCCTTGGAGGAGCTGCAGAACATCTTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTC
GATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCTGGCCC
GGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGC
CTTAGAGCAGCTCAAAGCCAAGCAACGCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGC
CGCCCGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACGGAGATGCAGCGGCTGACCAAGTACC
CCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGC
CGAGTGCTGCCGGGAAATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTC
AAGGACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGCAGCGACCCTATGCTGAGCGAGTTCA
AGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAGGACAA
GGCAGTGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTG
CTGCTCAAGTCCCATAGCCGGACACTGACGCCCACGCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGC
GGCTCACCTCCGCCATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACCTGGGA
CCAGGAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATC
ACTGAGACTGCCGGATCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCA
GCACCCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGACGTCTCCAGCTGATGC
CCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCC
ACGGCCCTTCGGAAAGTGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGC
CTCCTCGAGATGGGGATGGGGTCCCAGGGGGCGGCCCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGA
GAACCTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGA
CCCCTCCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221180 protein sequence
Red=Cloning site Green=Tags(s)

MASLSTWSSPAEPREMEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRR
PAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRT
RADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAE
RLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGL
SSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPD
REPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPD
TLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFL
DRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAES
RPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRL
KDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERL
LLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALI
TETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAA
TALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLR
PLLSQLGGNSVPQPGCT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199002
ORF Size 2781 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199002.2
RefSeq Size 3261 bp
RefSeq ORF 2784 bp
Locus ID 9138
UniProt ID Q92888
Cytogenetics 19q13.2
Protein Pathways Regulation of actin cytoskeleton, Vascular smooth muscle contraction
MW 104.1 kDa
Summary Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. provided by RefSeq, Jul 2008
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Documents
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"NM_199002" in other vectors (4)
SKU Description Size Price
RC221180L3 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,149.00
RC221180L4 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1, mGFP tagged 10 ug
$1,149.00
RG221180 ARHGEF1 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1 10 ug
$1,049.00
SC307847 ARHGEF1 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 1 10 ug
$935.00

Plasmid Map for RC221180

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221180
Circular map for RC221180

file-alt Citations

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