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SMC1L2 (SMC1B) (NM_148674) Human Tagged ORF Clone

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SKU
RC221101
SMC1B (Myc-DDK-tagged)-Human structural maintenance of chromosomes 1B (SMC1B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,075.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SMC1L2
Synonyms SMC1BETA; SMC1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221101 representing NM_148674
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCACCTGGAGCTGCTGCTTGTGGAAAATTTCAAGTCGTGGCGGGGCCGCCAGGTCATTGGCCCCT
TCCGGAGGTTCACCTGCATCATCGGCCCCAACGGCTCTGGAAAATCTAATGTAATGGATGCACTTAGTTT
TGTAATGGGAGAGAAAATAGCTAATTTAAGAGTGAAAAATATTCAAGAACTCATTCATGGAGCACATATT
GGAAAACCTATTTCTTCTTCTGCAAGTGTAAAAATTATATATGTGGAGGAAAGTGGCGAAGAGAAAACAT
TTGCAAGGATTATCCGAGGGGGATGCTCAGAATTTCGCTTTAATGATAATCTTGTGAGTCGTTCTGTTTA
CATTGCAGAGTTGGAAAAGATAGGCATAATAGTCAAAGCACAAAATTGTTTGGTTTTTCAGGGAACTGTA
GAGTCAATTTCAGTGAAGAAACCCAAAGAAAGGACCCAGTTTTTTGAGGAAATCAGCACTTCAGGAGAGC
TTATAGGAGAATATGAAGAAAAGAAAAGAAAGTTACAAAAAGCCGAAGAGGATGCACAGTTTAACTTTAA
TAAGAAAAAAAATATAGCGGCAGAGCGCAGACAAGCAAAATTAGAGAAGGAAGAGGCAGAACGTTACCAG
AGTCTCCTTGAAGAACTGAAAATGAACAAGATACAACTGCAGCTTTTTCAACTATACCATAATGAGAAAA
AGATTCATCTCCTGAACACCAAGTTAGAGCATGTGAATAGGGATTTGAGTGTCAAAAGAGAGTCTTTGTC
TCATCATGAAAACATAGTTAAAGCCAGGAAAAAGGAACATGGAATGCTAACTAGACAACTACAACAAACA
GAAAAAGAATTAAAATCGGTTGAAACCCTTTTAAATCAGAAGAGGCCTCAGTACATTAAAGCCAAAGAAA
ACACTTCTCACCACCTTAAGAAATTAGATGTGGCTAAGAAATCAATAAAGGACAGCGAAAAACAATGTTC
TAAACAGGAAGATGATATAAAAGCCCTGGAGACAGAGCTGGCTGATTTAGATGCTGCATGGAGAAGTTTT
GAAAAGCAGATTGAGGAAGAAATTTTACATAAAAAGCGAGACATTGAACTGGAAGCCAGTCAGCTGGATC
GTTATAAAGAACTTAAGGAACAAGTAAGAAAGAAAGTAGCTACAATGACTCAACAACTGGAAAAACTGCA
GTGGGAACAGAAGACAGATGAAGAAAGACTGGCATTTGAAAAGAGGAGGCATGGAGAAGTTCAGGGAAAT
CTAAAACAAATAAAAGAACAAATAGAAGATCATAAAAAACGAATAGAGAAGTTAGAGGAGTATACAAAGA
CATGCATGGATTGCTTGAAAGAGAAAAAACAGCAAGAGGAAACCCTAGTGGATGAAATTGAAAAAACAAA
ATCAAGAATGTCTGAATTTAATGAAGAATTGAATCTTATTAGAAGTGAATTGCAGAATGCTGGGATTGAT
ACCCATGAGGGAAAACGTCAGCAAAAGAGAGCAGAGGTTCTGGAACACCTTAAAAGACTGTACCCAGATT
CTGTGTTTGGAAGACTATTTGACCTGTGTCATCCTATTCATAAGAAATACCAGCTGGCTGTTACTAAGGT
TTTTGGCCGGTTCATCACTGCCATTGTTGTAGCCTCTGAAAAGGTAGCAAAAGATTGTATTCGATTTCTG
AAGGAGGAAAGAGCTGAACCTGAGACATTCCTCGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAA
GACTAAGGGAGCTTAAAGGCTGTAAAATGGTGATTGATGTCATAAAGACTCAGTTTCCTCAGCTGAAGAA
AGTGATTCAGTTTGTGTGTGGAAATGGTCTTGTTTGTGAGACTATGGAAGAAGCAAGGCATATTGCACTC
AGTGGACCTGAAAGACAGAAAACAGTAGCTCTTGATGGAACATTATTTTTAAAATCTGGAGTGATCTCTG
GAGGGTCAAGTGACTTAAAATACAAGGCTAGATGCTGGGATGAGAAAGAGTTAAAGAATCTAAGAGACAG
ACGAAGCCAGAAAATCCAAGAGCTAAAGGGTTTAATGAAGACACTCCGCAAAGAAACAGATTTGAAACAA
ATACAGACCCTGATACAGGGAACTCAAACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAGA
AGAAGCACCTTGTTGCTTTTTACCAGGAACAATCTCAGTTACAAAGTGAACTACTAAATATTGAGTCTCA
ATGTATTATGTTGAGTGAAGGAATCAAGGAACGACAACGAAGAATTAAAGAATTTCAAGAAAAGATAGAT
AAGGTAGAAGACGATATCTTCCAACACTTCTGTGAAGAAATTGGCGTGGAAAATATTCGTGAATTTGAGA
ACAAACATGTTAAACGGCAACAAGAAATTGATCAAAAAAGATTAGAATTTGAAAAACAAAAAACTCGGCT
TAATGTTCAACTTGAGTATAGTCGCAGTCACCTTAAGAAGAAACTGAATAAGATCAACACATTAAAAGAA
ACTATCCAGAAAGGTAGTGAAGATATTGATCACCTAAAGAAGGCTGAAGAAAACTGTCTGCAGACAGTGA
ATGAACTCATGGCAAAGCAGCAGCAACTTAAGGACATACGTGTCACTCAGAACTCCAGTGCCGAGAAAGT
TCAAACTCAAATTGAAGAGGAACGGAAGAAGTTTCTGGCTGTTGATAGGGAAGTGGGGAAATTGCAAAAA
GAAGTTGTAAGTATTCAAACTTCTCTGGAACAGAAACGATTAGAGAAGCATAACTTGCTGCTTGATTGCA
AAGTGCAAGACATTGAGATAATCCTTTTGTCGGGGTCACTGGATGACATCATTGAAGTGGAGATGGGAAC
TGAAGCAGAAAGTACCCAGGCAACAATTGATATCTATGAAAAAGAAGAAGCCTTTGAAATAGACTACAGC
TCTCTAAAAGAGGATTTGAAGGCTCTACAGTCTGATCAAGAAATCGAGGCCCACCTTAGGCTCTTATTGC
AGCAAGTAGCATCCCAGGAAGATATCTTACTGAAAACAGCAGCCCCAAACCTACGAGCACTGGAGAACTT
AAAGACTGTCAGAGACAAGTTTCAAGAGTCCACAGATGCTTTTGAGGCCAGCAGAAAGGAAGCCAGACTG
TGTAGGCAAGAGTTCGAGCAAGTGAAAAAAAGGAGATACGATCTTTTCACCCAGTGTTTTGAGCATGTCT
CAATCTCAATTGATCAAATCTACAAGAAGCTCTGCAGAAACAACAGCGCCCAAGCATTTCTTAGCCCAGA
GAACCCTGAAGAACCTTACTTGGAGGGAATTAGCTATAACTGTGTGGCCCCAGGCAAACGGTTTATGCCA
ATGGACAATTTGTCAGGGGGAGAAAAGTGTGTGGCAGCCTTGGCTCTCCTGTTTGCTGTGCACAGTTTTC
GTCCTGCCCCATTCTTTGTTTTAGATGAAGTGGATGCAGCCCTAGACAATACTAACATAGGCAAAGTGTC
AAGTTACATCAAAGAGCAAACTCAAGACCAGTTTCAGATGATAGTCATCTCCCTAAAAGAAGAGTTCTAT
TCCAGAGCCGACGCGCTGATCGGCATCTATCCTGAGTACGATGACTGCATGTTCAGCCGAGTTTTGACCC
TAGATCTTTCTCAGTATCCAGACACTGAAGGCCAAGAAAGCAGCAAGAGACACGGAGAGTCCCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221101 representing NM_148674
Red=Cloning site Green=Tags(s)

MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHI
GKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTV
ESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQ
SLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQT
EKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSF
EKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGN
LKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEFNEELNLIRSELQNAGID
THEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFL
KEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIAL
SGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQ
IQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKID
KVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKE
TIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQK
EVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYS
SLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARL
CRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQAFLSPENPEEPYLEGISYNCVAPGKRFMP
MDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQTQDQFQMIVISLKEEFY
SRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148674
ORF Size 3705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148674.3, NP_683515.3
RefSeq Size 4266 bp
RefSeq ORF 3708 bp
Locus ID 27127
UniProt ID Q8NDV3
Cytogenetics 22q13.31
Domains SMC_C, SMC_N
Protein Pathways Cell cycle, Oocyte meiosis
MW 143.7 kDa
Summary SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 PubMed 11564881).supplied by OMIM, Mar 2008
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"NM_148674" in other vectors (6)
SKU Description Size Price
RC221101L1 Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), Myc-DDK-tagged 10 ug
$1,375.00
RC221101L2 Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), mGFP tagged 10 ug
$1,375.00
RC221101L3 Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), Myc-DDK-tagged 10 ug
$1,375.00
RC221101L4 Lenti ORF clone of Human structural maintenance of chromosomes 1B (SMC1B), mGFP tagged 10 ug
$1,375.00
RG221101 SMC1B (tGFP-tagged) - Human structural maintenance of chromosomes 1B (SMC1B) 10 ug
$1,275.00
SC315290 SMC1B (untagged)-Human structural maintenance of chromosomes 1B (SMC1B) 10 ug
$1,183.00

Plasmid Map for RC221101

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221101
Circular map for RC221101

file-alt Citations

Identification of multiple gene mutations accounts for a new genetic architecture of primary ovarian insufficiency
Bouilly, Beau, Barraud et al
J. Clin. Endocrinol. Metab. (2016)
Cited by 147
AI Summary: OriGene products, specifically the pCMV6 (Myc-Flag tagged) plasmids for hSOHLH1, hFIGLA, hSMC1-ß, and hREC8, were used in this study to express and analyze candidate gene variants implicated in primary ovarian insufficiency.
Snippet: Plasmids pCMV6 (Myc-Flag tagged)-hSOHLH1, -hFIGLA,- hSMC1-ß and -hREC8 were purchased from Origene. pGEMT- hLHX8 was purchased from Sino Biological Inc. and the cDNA was subcloned into EcoRI and NotI sites of pCMV-HA vector. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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