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LPPR4 (PLPPR4) (NM_014839) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC220842
LPPR4 (Myc-DDK-tagged)-Human lipid phosphate phosphatase-related protein type 4 (LPPR4), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $699.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LPPR4
Synonyms LPPR4; LPR4; PHP1; PRG-1; PRG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220842 representing NM_014839
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGCGCTGGCTCCAGCGGTGGCCGCGGGGAATGTGACATCAGCGGCGCCGGGCGCTTGGGGCTGG
AGGAGGCAGCTCGCCTCAGCTGCGCTGTGCACACCTCGCCCGGGGGAGGACGCAGACCCGGGCAGGCGGC
AGGGATGTCGGCGAAGGAGAGGCCAAAGGGCAAAGTGATCAAGGACAGCGTCACCCTCCTGCCCTGCTTT
TATTTCGTCGAGTTGCCTATATTGGCATCATCGGTGGTTAGCCTCTATTTCCTCGAACTCACAGATGTCT
TCAAACCTGTGCACTCTGGATTTAGCTGCTATGACCGGAGTCTTAGCATGCCGTACATTGAACCAACCCA
GGAGGCAATTCCATTCCTCATGTTGCTTAGCTTGGCTTTTGCTGGACCTGCAATTACGATTATGGTAGGA
GAAGGAATTCTCTACTGTTGCCTCTCCAAAAGAAGAAATGGGGTCGGACTAGAGCCCAACATTAATGCTG
GAGGCTGCAACTTCAATTCCTTCCTCAGACGAGCTGTCAGATTCGTTGGTGTTCATGTATTTGGATTATG
CTCTACAGCTCTCATTACAGATATCATACAGCTGTCCACAGGATATCAAGCACCTTACTTTCTGACTGTG
TGCAAACCAAACTATACCTCTCTGAATGTATCTTGCAAAGAAAATTCCTACATTGTGGAAGATATTTGCT
CAGGATCTGACCTCACAGTTATCAACAGTGGCAGAAAGTCCTTCCCTTCTCAACATGCAACCCTTGCTGC
CTTTGCAGCTGTGTATGTTTCGATGTACTTCAATTCCACATTAACGGATTCCTCTAAGCTTCTGAAACCT
CTCTTGGTCTTCACATTTATCATCTGTGGAATAATCTGCGGGCTAACACGGATAACTCAGTATAAGAACC
ACCCAGTTGATGTCTATTGTGGCTTTTTAATAGGAGGAGGAATTGCACTGTACTTGGGCTTGTATGCTGT
GGGGAATTTCCTGCCCAGTGATGAGAGTATGTTTCAGCACAGAGACGCCCTCAGGTCTCTGACAGACCTC
AATCAAGATCCCAACCGACTTTTATCTGCTAAAAATGGTAGCAGCAGTGATGGAATTGCTCATACAGAAG
GCATCCTCAACCGAAACCACAGAGATGCTAGCTCTCTGACAAATCTCAAAAGAGCAAATGCTGATGTGGA
AATCATTACTCCACGGAGCCCCATGGGGAAGGAGAACATGGTTACCTTCAGCAATACCTTGCCGCGAGCC
AATACCCCATCTGTAGAAGACCCTGTCAGAAGAAATGCGAGCATTCATGCCTCTATGGATTCCGCTCGAT
CAAAGCAGCTCCTCACCCAGTGGAAGAATAAGAATGAAAGTCGAAAGTTGTCCTTGCAAGTTATAGAGCC
TGAGCCTGGGCAGTCACCACCCAGATCCATAGAAATGAGGTCAAGCTCAGAGCCATCGAGGGTAGGGGTG
AATGGAGACCACCATGGTCCTGGCAATCAGTACCTCAAAATCCAGCCTGGCGCTGTCCCCGGATGTAACA
ACAGCATGCCTGGAGGGCCAAGAGTGTCCATTCAGTCCCGTCCTGGGTCCTCACAGTTGGTGCACATCCC
TGAGGAGACTCAGGAAAACATAAGCACCTCCCCCAAAAGCAGCTCTGCTCGGGCCAAGTGGTTAAAAGCT
GCTGAAAAGACTGTGGCCTGTAACAGAAGCAACAGCCAGCCCCGAATCATGCAAGTCATAGCCATGTCCA
AGCAGCAGGGTGTCCTCCAAAGCAGCCCCAAGAACACTGAAGGCAGCACGGTCTCCTGCACTGGCTCCAT
CCGCTATAAAACCTTGACAGACCATGAGCCCAGTGGGATAGTGAGGGTTGAGGCTCACCCAGAGAACAAC
AGGCCCATCATACAGATCCCGTCCACTGAAGGTGAAGGCAGTGGCTCCTGGAAGTGGAAAGCCCCTGAAA
AGGGCAGCCTTCGCCAAACTTACGAGCTCAACGATCTCAACAGGGACTCAGAAAGCTGTGAGTCTCTGAA
AGACAGCTTTGGTTCTGGAGATCGCAAGAGAAGCAACATTGATAGCAATGAGCATCACCACCACGGAATT
ACCACCATCCGCGTCACCCCAGTAGAGGGCAGCGAAATTGGCTCAGAGACGCTGTCCATTTCTTCTTCCC
GCGACTCCACCCTGCGGAGAAAGGGCAATATCATTCTAATCCCTGAAAGAAGCAACAGCCCCGAAAACAC
TAGAAATATCTTCTACAAAGGAACCTCCCCCACACGGGCTTATAAGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220842 representing NM_014839
Red=Cloning site Green=Tags(s)

MQRAGSSGGRGECDISGAGRLGLEEAARLSCAVHTSPGGGRRPGQAAGMSAKERPKGKVIKDSVTLLPCF
YFVELPILASSVVSLYFLELTDVFKPVHSGFSCYDRSLSMPYIEPTQEAIPFLMLLSLAFAGPAITIMVG
EGILYCCLSKRRNGVGLEPNINAGGCNFNSFLRRAVRFVGVHVFGLCSTALITDIIQLSTGYQAPYFLTV
CKPNYTSLNVSCKENSYIVEDICSGSDLTVINSGRKSFPSQHATLAAFAAVYVSMYFNSTLTDSSKLLKP
LLVFTFIICGIICGLTRITQYKNHPVDVYCGFLIGGGIALYLGLYAVGNFLPSDESMFQHRDALRSLTDL
NQDPNRLLSAKNGSSSDGIAHTEGILNRNHRDASSLTNLKRANADVEIITPRSPMGKENMVTFSNTLPRA
NTPSVEDPVRRNASIHASMDSARSKQLLTQWKNKNESRKLSLQVIEPEPGQSPPRSIEMRSSSEPSRVGV
NGDHHGPGNQYLKIQPGAVPGCNNSMPGGPRVSIQSRPGSSQLVHIPEETQENISTSPKSSSARAKWLKA
AEKTVACNRSNSQPRIMQVIAMSKQQGVLQSSPKNTEGSTVSCTGSIRYKTLTDHEPSGIVRVEAHPENN
RPIIQIPSTEGEGSGSWKWKAPEKGSLRQTYELNDLNRDSESCESLKDSFGSGDRKRSNIDSNEHHHHGI
TTIRVTPVEGSEIGSETLSISSSRDSTLRRKGNIILIPERSNSPENTRNIFYKGTSPTRAYKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014839
ORF Size 2289 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014839.4, NP_055654.2
RefSeq Size 4988 bp
RefSeq ORF 2148 bp
Locus ID 9890
UniProt ID Q7Z2D5
Cytogenetics 1p21.3-p21.2
Protein Families Transmembrane
MW 82.8 kDa
Summary The protein encoded by this gene belongs to the lipid phosphate phosphatase (LPP) family. LPPs catalyze the dephosphorylation of a number of bioactive lipid mediators that regulate a variety of cell functions. This protein is specifically expressed in neurons. It is located in the membranes of outgrowing axons and has been shown to be important for axonal outgrowth during development and regenerative sprouting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Oct 2009
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Documents
Resources
"NM_014839" in other vectors (4)
SKU Description Size Price
RC220842L3 Lenti ORF clone of Human lipid phosphate phosphatase-related protein type 4 (LPPR4), transcript variant 1, Myc-DDK-tagged 10 ug
$999.00
RC220842L4 Lenti ORF clone of Human lipid phosphate phosphatase-related protein type 4 (LPPR4), transcript variant 1, mGFP tagged 10 ug
$999.00
RG220842 LPPR4 (tGFP-tagged) - Human lipid phosphate phosphatase-related protein type 4 (LPPR4), transcript variant 1 10 ug
$899.00
SC314642 LPPR4 (untagged)-Human lipid phosphate phosphatase-related protein type 4 (LPPR4), transcript variant 1 10 ug
$769.00

Plasmid Map for RC220842

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220842
Circular map for RC220842

file-alt Citations

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