PEAK3 (NM_198532) Human Tagged ORF Clone

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SKU
RC220828
C19orf35 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 35 (C19orf35)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PEAK3
Synonyms C19orf35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220828 representing NM_198532
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGCCCGGAGCCCCCCACAGAGCCCCCCGAGCCCGACAACCCCACCTGGTCGACTCAGCCCACGT
ATAGCAACCTTGGTCAGATCCGTGCCCACCTGCTGCCCTCCAAGGCCTGCCGCCTCCGGACCCCTGGGTC
CCTCTCCACCAACCCAGAGCCCCTGCCCCCACCCCTGCCCAAGAAGATCCTAACCCGGACCCAGTCACTG
CCCACCCGCAGGACCCTCCATCCCAGCTCCATCCAAGTACAGCCGCCTCGGAGACCCTTTCTGGGGTCCC
ACAGTGTGGACAAGAGCCAGGCTGCAGTGGGACCAGCCTGTCTCCCTGCAGAGCTGACCTTTGGCCCGGC
TGACGCCCCACTGGGCCTCTCCCTCCGCGATCTGCACAGCCCGGAGGCTGTGCACACTGCACTGGCTGCG
CGGCAGCTGCAGGGCCTCCGTACCATCTATGCCCGGCTCCGTGCCCGGCTCATGGGGGGCCACCCCGGGC
CCTGCCACCCCGGCCACAGCTTCCGCCTCCTAGACAGCTCACCCTGCGCAGAGAGCGGGGACGCCCTGTA
TTACCGCGTGGTGCGCGCGCACGAGGACGCCTGGCACATCCTGGTCGCCAAGGTGCCCAAGCCCGGGGCG
GACGTGCCCCACCCGTGGGGCCTGGAGCTGCAGGCCTCCCTGTCTCCACACTTCAATCTGCAGGGGCTGT
GTGGCCTGGTGCCTGAAGGCACACTGCCCGGGGCGCCCTGGAGAGGCGCAGTGGCGCTGGCAGCCGAGGT
TCCAGAGCGCACGGTGGCGCAGTGGCTGGCGGAGGCCTGCACGCAGCCGCCGGAGGAGTTCGTGTGGGCT
GTGGCCCTGCTGCTGCTGCAGCTGAGCGCGGCCCTGAAGTTCCTGGAGGCGTGGGGCGCGGCCCTAGTCG
AGTTGCGGCCGGAGAACTTGCTGCTGGTGGCACCTCGGGGCTGTGCGACGACGGGGCCCCCACGCCTGCT
CCTCACTGACTTTGGCCGCGTCTGTCTGCAGCCCCCTGGACCCCCGGGATCCCCGGGCCCCCACGCGCCG
CAGCTGGGCAGCCTCCTCCGAGCGCTGCTCAGCCTTGCTGCGCCCTCGACCACGCCTTTGGCCGCGGGCC
TGGAGCTCCTGGCAGCACAGCTGACCCGCTTGCGGCCCTCGGCGTCCCGGACGCGGGGCGCGCTGCAGGC
GCTGCTCTGGGGGCCCGGGCCTGAGCTGCGCGGCCGCGGAGCACCGCTTGGTCCCTGGCTCCGAGCGCTC
GGGCCCTGGCTGCGGGTGCGCCGCGGGCTGCTGGTCCTGCGCCTAGCAGAGCGGGCCGCAGGTGGGGAAG
CTCCCAGCCTCGAGGACTGGCTGTGTTGCGAATACCTGGCCGAGGCCACCGAGTCCTCGATGGGCCAGGC
CCTGGCGCTGCTGTGGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC220828 representing NM_198532
Red=Cloning site Green=Tags(s)

MSSPEPPTEPPEPDNPTWSTQPTYSNLGQIRAHLLPSKACRLRTPGSLSTNPEPLPPPLPKKILTRTQSL
PTRRTLHPSSIQVQPPRRPFLGSHSVDKSQAAVGPACLPAELTFGPADAPLGLSLRDLHSPEAVHTALAA
RQLQGLRTIYARLRARLMGGHPGPCHPGHSFRLLDSSPCAESGDALYYRVVRAHEDAWHILVAKVPKPGA
DVPHPWGLELQASLSPHFNLQGLCGLVPEGTLPGAPWRGAVALAAEVPERTVAQWLAEACTQPPEEFVWA
VALLLLQLSAALKFLEAWGAALVELRPENLLLVAPRGCATTGPPRLLLTDFGRVCLQPPGPPGSPGPHAP
QLGSLLRALLSLAAPSTTPLAAGLELLAAQLTRLRPSASRTRGALQALLWGPGPELRGRGAPLGPWLRAL
GPWLRVRRGLLVLRLAERAAGGEAPSLEDWLCCEYLAEATESSMGQALALLWD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198532
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198532.3
RefSeq Size 2565 bp
RefSeq ORF 1422 bp
Locus ID 374872
UniProt ID Q6ZS72
Cytogenetics 19p13.3
MW 50.3 kDa
Summary Probable catalytically inactive kinase (Probable). Interacts with CRK-II and antagonizes CRK-II-signaling. Prevents the formation of CRK-II-dependent membrane ruffling and lamellipodia-like extensions (PubMed:31311869).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198532" in other vectors (4)
SKU Description Size Price
RC220828L3 Lenti ORF clone of Human chromosome 19 open reading frame 35 (C19orf35), Myc-DDK-tagged 10 ug
$757.00
RC220828L4 Lenti ORF clone of Human chromosome 19 open reading frame 35 (C19orf35), mGFP tagged 10 ug
$757.00
RG220828 C19orf35 (tGFP-tagged) - Human chromosome 19 open reading frame 35 (C19orf35) 10 ug
$657.00
SC307740 C19orf35 (untagged)-Human chromosome 19 open reading frame 35 (C19orf35) 10 ug
$503.00

Plasmid Map for RC220828

Download sequence for

Cloning scheme for RC220828

Circular map for RC220828

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