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AGBL1 (NM_152336) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC220826
AGBL1 (Myc-DDK-tagged)-Human ATP/GTP binding protein-like 1 (AGBL1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $928.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AGBL1
Synonyms CCP4; FECD8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220826 representing NM_152336
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAGCAAGGGTGGCAGTGAAGCTCTTCTGCAGACCCTGGTAGACACAGCGAGGACAGCTCCTCCAG
ACTATGACATCCTCCTGCCTCTCTTCCGGCTGCTGGCCAAAGTTGGCCTAAGAGATAAAAAGATTGGACG
GAAGGCCCTAGAATTGGAAGCACTTGATGTGACATTGATTCTGGCCAGGAAGAACCTATCCCATGGCCAG
AATCTCCTCCACTGTCTCTGGGCTCTGCGTGTGTTTGCCTCCAGTGTCTCCATGGGAGCCATGCTGGGAA
TTAATGGAGCCATGGAACTGCTTTTCAAGGTTATTACTCCTTACACCCGAAAGCGCACCCAAGCAATCAG
GGCAGCCACTGAAGTTTTGGCAGCATTGCTGAAATCCAAGTCGAACGGCCGCAGAGCAGTGAACCGAGGC
TACGTCACCAGCCTGCTCGGGCTGCACCAGGACTGGCACAGCCATGACACAGCCAACGCCTACGTGCAGA
TCCGACGGGGCTTGCTGCTCTGCCTCAGGCACATTGCTGCCCTCCGGTCCGGCAGGGAGGCCTTCCTGGC
AGCACAGGGCATGGAGATCCTCTTCAGCACCACACAGAACTGCCTGGATGACAAGAGCATGGAGCCCGTC
ATCTCTGTGGTGCTTCAGATCCTGAGGCAGTGCTACCCTACGAGTCCACTTCCCTTGGTCACAGCCAGCA
GTGCCTATGCCTTCCCGGTCCCCGGGTGCATCACCACTGAACCTCCACATGATCTACCTGAAGAGGATTT
TGAAGATGATGGCGATGATGAAGTGGACAAAGACTCTGATACTGAAGATGGGAAAGTGGAAGATGATGAC
TTGGAAACAGACGTGAACAAGCTGAGTTCCAAACCTGGTCTTGACCGACCTGAAGAGGAACTGATGCAAT
ATGAGGTGATGTGTCTTGAGCTCTCCTATAGCTTTGAGGAACTGCAGTCCAAACTTGGAGATGATTTGAA
CTCTGAAAAGACTCAGTATGCCAATCACCACCACATTCCAGCCGCTGCCTCCTCAAAACAGCATTGCTAC
AGCAAGGACCAAAGCTCCTGTGGGCAAGAAAGAGAATATGCTGTCCAGACTTCCCTTCTGTGCAGGGTGA
AGACGGGAAGGTCCACTGTGCATCTAGGCTCCAAAAAAAATCCTGGAGTGAACCTGTACCAAAATGTGCA
ATCCAATAGTCTCAGGAGAGATTCTTCTGAAAGTGAAATCCCTGACATTCAGGCTTCCCCGAAAGCAGAT
GCCTGGGACGTAGATGCAATTTTCTGCCCAAGGATGAGTGCCTCCTTTTCTAATTCCACTAGGACTAGAG
AAGTTGTCAAAGTAATAGATAAGCTTCTGCAGACACATCTGAAGCGTGTCCCTTTCCACGATCCCTATCT
TTATATGGCCAAAGCCAGAAGAACCAGCTCTGTGGTGGACTTCAAGATGATGGCATTTCCTGATGTCTGG
GGACACTGTCCCCCTCCCACCACCCAGCCTATGTTGGAACGAAAATGTGGAGTCCAAAGGATCAGGATAT
TTGAGGATATTCGGAGGCTCATCCAGCCAAGTGATGTTATAAATAAAGTTGTCTTCAGTTTAGATGAGCC
TTGGCCTTTGCAAGACAATGCTTCCAATTGTTTACGGTTCTTCTCCAAATTTGAGTCAGGAAATCTTCGC
AAAGCCATCCAAGTGCGTGAGTTCGAGTATGACTTGCTGGTCAACGCAGATGTGAATAGCACCCAGCACC
AGCAGTGGTTCTATTTCAAAGTGAGCGGTATGCAGGCGGCCATCCCTTACCACTTCAACATCATCAACTG
TGAGAAGCCCAACAGCCAGTTTAATTATGGGATGCAGCCCACCCTATATTCTGTGAAGGAGGCTCTTCTT
GGCAAACCCACCTGGATAAGGACAGGCCATGAAATATGTTATTACAAAAATCATTATCGCCAGAGTACAG
CTGTTGCAGGCGGAGCATCTGGGAAGTGCTACTATACCCTCACCTTTGCTGTCACCTTCCCACACAGTGA
GGATGTCTGCTACCTGGCCTACCACTATCCCTATACCTACACAGCCCTCATGACTCATCTTGACATCCTG
GAAAAGAGTGTCAACCTCAAAGAGGTCTACTTCCGGCAAGATGTTCTCTGCCAGACGCTGGGAGGGAATC
CGTGTCCCTTGGTGACCATCACGGCCATGCCTGAGTCCAACAGTGATGAGCATCTAGAGCAGTTCCGACA
TCGTCCATATCAGGTGATCACTGCTCGAGTTCATCCAGGAGAGAGCAATGCCAGTTGGGTGATGAAGGGT
ACCTTGGAGTTCCTGGTCAGCAGTGACCCTGTGGCTAGGCTCTTGAGGGAAAACTTCATCTTCAAGATCA
TACCCATGCTCAACCCAGATGGTGTCATCAACGGCAACCACAGATGCTCACTGAGCGGGGAAGATTTGAA
CAGACAATGGCTTTCTCCCAGTGCTCATCTGCAGCCAACCATTTACCATGCCAAAGGCCTCCTCTACCAC
CTGAGCAGCATTGGCCGAAGTCCCGTGGTTTTCTGTGACTTCCATGGCCACTCCCAAAAGAAGAATGTGT
TCCTTTATGGCTGTAGCATCAAGGAAACCTTGTGGCAAGCAGCATGCACTGTGGGCACATCTACTATCCT
AGAGGAGGTCAACTACAGGACTCTTCCCAAAATCCTTGATAAGCTAGCACCAGCATTCACAATGAGCAGC
TGCAGCTTTCTCGTGGAGAAATCTCGAGCTTCCACGGCCCGGGTGGTGGTGTGGAGAGAGATGGGGGTGT
CCAGAAGCTACACCATGGAAAGCAGCTACTGTGGCTGCAACCAGGGCCCTTATCAGTGTACACAGAGGCT
GTTGGAGAGGACAAAGAATGAAAGGGCTCACCCAGTGGATGGCCTTCAGGGTCTACAGTTTGGTACCAGA
GAACTGGAGGAGATGGGAGCCATGTTCTGTTTGGGCCTCCTCATCCTGGAGCTCAAATCTGCCAGCTGCA
GCCATCAGCTCCTGGCTCAAGCTGCAACTCTGCTGAGTGCTGAGGAGGACGCTCTGGACCAGCACCTCCA
ACGATTAAAATCATCCAATTTCCTGCCAAAGCATATTTGGTTTGCTTACCACTTTTTTGCCATTACAAAC
TTTTTCAAGATGAACCTTCTTCTGCATGTCTCCCCAGTTTGTGACACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220826 representing NM_152336
Red=Cloning site Green=Tags(s)

MISKGGSEALLQTLVDTARTAPPDYDILLPLFRLLAKVGLRDKKIGRKALELEALDVTLILARKNLSHGQ
NLLHCLWALRVFASSVSMGAMLGINGAMELLFKVITPYTRKRTQAIRAATEVLAALLKSKSNGRRAVNRG
YVTSLLGLHQDWHSHDTANAYVQIRRGLLLCLRHIAALRSGREAFLAAQGMEILFSTTQNCLDDKSMEPV
ISVVLQILRQCYPTSPLPLVTASSAYAFPVPGCITTEPPHDLPEEDFEDDGDDEVDKDSDTEDGKVEDDD
LETDVNKLSSKPGLDRPEEELMQYEVMCLELSYSFEELQSKLGDDLNSEKTQYANHHHIPAAASSKQHCY
SKDQSSCGQEREYAVQTSLLCRVKTGRSTVHLGSKKNPGVNLYQNVQSNSLRRDSSESEIPDIQASPKAD
AWDVDAIFCPRMSASFSNSTRTREVVKVIDKLLQTHLKRVPFHDPYLYMAKARRTSSVVDFKMMAFPDVW
GHCPPPTTQPMLERKCGVQRIRIFEDIRRLIQPSDVINKVVFSLDEPWPLQDNASNCLRFFSKFESGNLR
KAIQVREFEYDLLVNADVNSTQHQQWFYFKVSGMQAAIPYHFNIINCEKPNSQFNYGMQPTLYSVKEALL
GKPTWIRTGHEICYYKNHYRQSTAVAGGASGKCYYTLTFAVTFPHSEDVCYLAYHYPYTYTALMTHLDIL
EKSVNLKEVYFRQDVLCQTLGGNPCPLVTITAMPESNSDEHLEQFRHRPYQVITARVHPGESNASWVMKG
TLEFLVSSDPVARLLRENFIFKIIPMLNPDGVINGNHRCSLSGEDLNRQWLSPSAHLQPTIYHAKGLLYH
LSSIGRSPVVFCDFHGHSQKKNVFLYGCSIKETLWQAACTVGTSTILEEVNYRTLPKILDKLAPAFTMSS
CSFLVEKSRASTARVVVWREMGVSRSYTMESSYCGCNQGPYQCTQRLLERTKNERAHPVDGLQGLQFGTR
ELEEMGAMFCLGLLILELKSASCSHQLLAQAATLLSAEEDALDQHLQRLKSSNFLPKHIWFAYHFFAITN
FFKMNLLLHVSPVCDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152336
ORF Size 3198 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152336.2, NP_689549.2
RefSeq Size 3493 bp
RefSeq ORF 3339 bp
Locus ID 123624
UniProt ID Q96MI9
Cytogenetics 15q25.3
MW 120.1 kDa
Summary Polyglutamylation is a reversible posttranslational modification catalyzed by polyglutamylases that results in the addition of glutamate side chains on the modified protein. This gene encodes a glutamate decarboxylase that catalyzes the deglutamylation of polyglutamylated proteins. Mutations in this gene result in dominant late-onset Fuchs corneal dystrophy. provided by RefSeq, Nov 2013
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Documents
Resources
"NM_152336" in other vectors (4)
SKU Description Size Price
RC220826L3 Lenti ORF clone of Human ATP/GTP binding protein-like 1 (AGBL1), Myc-DDK-tagged 10 ug
$1,228.00
RC220826L4 Lenti ORF clone of Human ATP/GTP binding protein-like 1 (AGBL1), mGFP tagged 10 ug
$1,228.00
RG220826 AGBL1 (tGFP-tagged) - Human ATP/GTP binding protein-like 1 (AGBL1) 10 ug
$1,128.00
SC318477 AGBL1 (untagged)-Human ATP/GTP binding protein-like 1 (AGBL1) 10 ug
$929.00

Plasmid Map for RC220826

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220826
Circular map for RC220826

file-alt Citations

ER proteins decipher the tubulin code to regulate organelle distribution
Zheng, Obara, Szczesna et al
Nature (2022) 601 (7891), 132-138 DOI: 10.1038/s41586-021-04204-9
Cited by 164
AI Summary: OriGene products—specifically pCMV6_p180s-myc-Flag (RC218816), pCMV6_KTN1-myc-Flag (RC219832), pCMV6_TTLL4-myc-Flag (RC205206), and pCMV6_CCP1-myc-Flag (RC220826)—were used to express key ER and microtubule-modifying proteins, enabling the study of their roles in organelle positioning and ER-microtubule interactions in this research.
Snippet: … pCMV6_p180s-myc-Flag (RC218816), pCMV6_KTN1-myc-Flag (RC219832), pCMV6_TTLL4-myc-Flag (RC205206) and pCMV6_CCP1-myc-Flag (RC220826) were obtained from Origene Technologies. pcDNA3.1_TTLL6-Flag (OHu07095), pcDNA3.1_CCP5-Flag (Ohu28493), pcDNA3.1_CCP6-Flag (OHu24335) and pcDNA3.1_p180L (OHu24745) were obtained from GenScript. Mutants of CLIMP63, p180s;pCMV6_TTLL4-myc-Flag (RC205206) and pCMV6_CCP1-myc-Flag (RC220826) were obtained from Origene Technologies. pcDNA3.1_TTLL6-Flag (OHu07095), pcDNA3.1_CCP5-Flag (Ohu28493), pcDNA3.1_CCP6-Flag (OHu24335) and pcDNA3.1_p180L (OHu24745) were obtained from GenScript. …
Showing 1-1 of 1 papers.
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