Chorein (VPS13A) (NM_033305) Human Tagged ORF Clone
- TrueORF®
SKU
RC220762
VPS13A (Myc-DDK-tagged)-Human vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant A
$3,857.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Chorein |
| Synonyms | CHAC; CHOREIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_033305 |
| ORF Size | 9522 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_033305.3 |
| RefSeq Size | 11262 bp |
| RefSeq ORF | 9525 bp |
| Locus ID | 23230 |
| UniProt ID | Q96RL7 |
| Cytogenetics | 9q21.2 |
| MW | 360.1 kDa |
| Summary | The protein encoded by this gene may control steps in the cycling of proteins through the trans-Golgi network to endosomes, lysosomes and the plasma membrane. Mutations in this gene cause the autosomal recessive disorder, chorea-acanthocytosis. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_033305" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220762L1 | Lenti ORF clone of Human vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant A, Myc-DDK-tagged | 10 ug |
$4,157.00
|
|
| RG220762 | VPS13A (tGFP-tagged) - Human vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant A | 10 ug |
$4,057.00
|
|
| SC305608 | VPS13A (untagged)-Human vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant A | 10 ug |
$4,115.00
|
|
Citations
VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites
Kumar, Leonzino, Hancock-Cerutti et al
J. Cell Biol. (2018)
Kumar, Leonzino, Hancock-Cerutti et al
J. Cell Biol. (2018)
AI Summary: OriGene contributed to this study by providing human VPS13A (transcript variant 1A) and VPS13C (transcript variant 2A) ORF constructs, which were essential for investigating the localization and lipid transport functions of these proteins.
Snippet: Halo tag ligands JF549 and JF647 were a kind gift from L. Lavis (Janelia Farm, Ashburn, VA). Human VPS13A (transcript variant 1A) and VPS13C (transcript variant 2A) ORFs were purchased from Origene. The following constructs were kind gifts: mito-BFP and mCherry-Rab7a from G. Voeltz (University of Colorado Boulder, Boulder, CO;49151 and 61804, respectively;Addgene),
Chorein, the protein responsible for chorea-acanthocytosis, interacts with β-adducin and β-actin
Shiokawa, Nakamura, Sameshima et al
Biochem. Biophys. Res. Commun. (2013)
Shiokawa, Nakamura, Sameshima et al
Biochem. Biophys. Res. Commun. (2013)
Cited by 44
AI Summary: OriGene’s pCMV6 vector containing a Myc-DDK-tagged Homo sapiens VPS13A (chorein) ORF clone was used to generate chorein-overexpressing HEK293 cell lines, enabling the study of chorein’s interactions with β-adducin and β-actin in this paper.
Snippet: To generate stable cell lines overexpressing chorein, HEK293 cells were transfected with pCMV6 vector, containing an ORF clone of Homo sapiens VPS13A (transcript variant A) with a Myc-DDK tag at the C-terminus and a neomycin selectable cassette (Origene, Rockville
Showing 1-2 of 2 papers.
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