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ZNF470 (NM_001001668) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC220720
ZNF470 (Myc-DDK-tagged)-Human zinc finger protein 470 (ZNF470). Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
$589.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF470
Synonyms CZF-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220720 ORF sequence, codon optimized.
Due to the complexity of NM_001001668, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGCCAGGAAGAAGTCGAGGTTGCGGGAATAAAGCTGTGCAAGGCCATGAGTCTGGGAAGCCTCA
CCTTCACTGACGTGGCCATCGACTTCTCACAGGACGAGTGGGAATGGCTCAATCTGGCACAAAGAAGTTT
GTATAAAAAGGTCATGCTGGAGAATTATCGAAACCTCGTGTCTGTGGGTCTGTGCATCAGTAAACCAGAT
GTAATCTCATTGTTGGAACAGGAAAAGGATCCCTGGGTTATTAAGGGAGGCATGAATCGCGGGCTGTGTC
CTGATTTGGAGTGTGTTTGGGTGACAAAAAGCCTCTCCCTGAACCAGGACATTTACGAGGAGAAATTGCC
TCCTGCGATCATTATGGAACGGCTGAAGAGTTACGATCTCGAATGTTCCACCCTGGGCAAGAACTGGAAG
TGTGAGGATTTGTTCGAGAGGGAACTGGTCAACCAAAAAACCCACTTCCGGCAGGAGACTATTACTCATA
TCGATACTCTCATTGAAAAGAGGGATCACTCTAACAAGTCAGGCACCGTATTTCATCTCAATACTCTTAG
TTATATCAAACAGATATTCCCTATGGAGGAGAGGATCTTTAACTTTCACACTGACAAAAAGTCTCTGAAA
ACCCACAGCGTTGTAAAAAAGCACAAGCAGGACCGAGGCGAGAAGAAGCTGCTTAAATGCAATGACTGTG
AGAAGATATTCTCAAAGATCTCAACACTGACTCTGCATCAAAGAATCCATACCGGTGAGAAGCCATACGA
GTGCATTGAGTGTGGCAAAGCCTTCTCCCAGTCAGCACACCTTGCACAGCACCAGCGCATTCACACCGGG
GAGAAGCCCTTCGAATGCACCGAGTGCGGAAAGGCTTTCTCTCAGAACGCACACCTCGTACAGCACCAGA
GGGTGCACACTGGGGAGAAGCCCTACCAATGTAAACAGTGCAACAAAGCATTTTCTCAACTCGCCCACCT
CGCTCAGCACCAGAGAGTCCACACCGGTGAAAAACCCTACGAGTGTATTGAATGTGGCAAGGCCTTTTCT
GACTGTTCCTCACTGGCACATCATCGCAGAATTCATACTGGAAAACGACCATACGAGTGCATTGACTGCG
GCAAGGCGTTCCGGCAGAACGCTAGTCTCATCCGCCATCGACGCTACTACCATACAGGGGAGAAACCTTT
TGATTGTATTGACTGCGGTAAGGCATTTACCGATCATATCGGCCTGATCCAGCACAAAAGAATCCATACT
GGGGAGAGGCCATATAAGTGTAATGTCTGCGGAAAAGCCTTTTCCCACGGGAGCAGTCTCACAGTTCACC
AGCGCATCCACACCGGAGAGAAGCCATACGAATGTAACATCTGCGAAAAAGCGTTCTCACATCGAGGCTC
ACTTACACTGCACCAAAGAGTCCATACTGGCGAAAAGCCGTACGAGTGCAAGGAATGTGGAAAAGCTTTT
AGACAGTCTACCCATCTGGCCCACCATCAACGCATTCACACCGGAGAAAAGCCTTACGAATGTAAGGAGT
GCAGTAAAACATTTTCACAGAACGCCCATCTCGCCCAGCACCAGAAAATCCATACCGGAGAAAAGCCGTA
TGAGTGTAAGGAGCGCGGAAAGGCTTTTTCCCAAATCGCCCATCTGGTGCAACACCAAAGGGTACATACG
GGAGAAAAGCCATATGAGTGCATTGAATGCGGGAAGGCCTTCTCAGACGGCAGTTACCTGGTGCAGCACC
CACGGCTGCACTCTGGTAAGCGGCCTTACGAATGCCTGGAGTGCGGTAAGGCCTTTAGGCAGCGGGCGAG
CTTGATTTGCCACCAACGCTGCCACACTGGAGAGAAGCCTTATGAGTGCAATGTATGTGGGAAGGCCTTC
TCCCACAGGAAATCCTTGACTCTGCACCAGCGCATCCACACTGGGGAAAAGCCTTACGAATGTAAGGAGT
GTAGCAAGGCTTTCAGCCAGGTCGCACATCTGACGCTCCACAAGCGGATCCACACTGGCGAGCGACCTTA
CGAATGCAAAGAATGCGGCAAAGCGTTTAGGCAGTCCGTACACCTGGCACATCATCAGAGGATACACACT
GGTGAGAGTTCCGTCATCCTCTCCTCTGCTCTGCCCTACCACCAGGTACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220720 representing NM_001001668
Red=Cloning site Green=Tags(s)

MKSQEEVEVAGIKLCKAMSLGSLTFTDVAIDFSQDEWEWLNLAQRSLYKKVMLENYRNLVSVGLCISKPD
VISLLEQEKDPWVIKGGMNRGLCPDLECVWVTKSLSLNQDIYEEKLPPAIIMERLKSYDLECSTLGKNWK
CEDLFERELVNQKTHFRQETITHIDTLIEKRDHSNKSGTVFHLNTLSYIKQIFPMEERIFNFHTDKKSLK
THSVVKKHKQDRGEKKLLKCNDCEKIFSKISTLTLHQRIHTGEKPYECIECGKAFSQSAHLAQHQRIHTG
EKPFECTECGKAFSQNAHLVQHQRVHTGEKPYQCKQCNKAFSQLAHLAQHQRVHTGEKPYECIECGKAFS
DCSSLAHHRRIHTGKRPYECIDCGKAFRQNASLIRHRRYYHTGEKPFDCIDCGKAFTDHIGLIQHKRIHT
GERPYKCNVCGKAFSHGSSLTVHQRIHTGEKPYECNICEKAFSHRGSLTLHQRVHTGEKPYECKECGKAF
RQSTHLAHHQRIHTGEKPYECKECSKTFSQNAHLAQHQKIHTGEKPYECKERGKAFSQIAHLVQHQRVHT
GEKPYECIECGKAFSDGSYLVQHPRLHSGKRPYECLECGKAFRQRASLICHQRCHTGEKPYECNVCGKAF
SHRKSLTLHQRIHTGEKPYECKECSKAFSQVAHLTLHKRIHTGERPYECKECGKAFRQSVHLAHHQRIHT
GESSVILSSALPYHQVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001001668
ORF Size 2151 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001001668.2, NM_001001668.3, NP_001001668.2
RefSeq Size 7194 bp
RefSeq ORF 2154 bp
Locus ID 388566
UniProt ID Q6ECI4
Cytogenetics 19q13.43
MW 82.7 kDa
Summary May be involved in transcriptional regulation.UniProtKB/Swiss-Prot Function
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"NM_001001668" in other vectors (2)
SKU Description Size Price
RG220720 ZNF470 (tGFP-tagged) - Human zinc finger protein 470 (ZNF470). Note: ORF is codon optimized 10 ug
$857.00
SC300253 ZNF470 (untagged)-Human zinc finger protein 470 (ZNF470) 10 ug
$723.00

Plasmid Map for RC220720

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220720
Circular map for RC220720

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