Leucyl cystinyl aminopeptidase (LNPEP) (NM_005575) Human Tagged ORF Clone

SKU RC220692

LNPEP (Myc-DDK-tagged)-Human leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Leucyl cystinyl aminopeptidase
• Synonyms: CAP; IRAP; P-LAP; PLAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220692 representing NM_005575; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATGTTTGAGGAAG; AACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAGCCTGATGAGGTGGAATATGA; GCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAGCATGAGATGGAGGAGGATGAAGAGGATTAT; GAGTCATCAGCAAAGCTGCTGGGCATGTCCTTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTG; GTTACAGGCAGAGCCCAGATGGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGT; CATCGTGGTTGCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGC; TGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTGTTTCCATGGG; CACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGCCTACACCCGAACCTAACCTC; GATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCTCTTCAGGTCACATGGAATATCATTCTTCAT; AGCACAGGTCATAATATTTCAAGAGTGACCTTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGA; TCCTGGAATATGCATATCATGGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGGCACAATTA; TACGTTGAAGATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTTTATGGCTTCTCCTACACA; GATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGATCTGCTTTTC; CTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCAAGATCATAAGGGATGAGCAATACACCGC; TTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGATGATGGACTTGTTCAGGATGAGTTTTCTGAG; AGTGTGAAGATGAGCACTTACTTGGTTGCTTTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAA; ATGGAACCCTGGTTTCTATATATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTGGAAACAAC; TGTGAAGCTTCTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTTAAGAAATTGGATTTGGTG; GCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGTTTGCTCACCTTCCGAGAGGAGACACTTC; TGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGGTGACTAAAATCATTGCTCATGAGCTGGC; CCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGGAATGACCTATGGCTAAATGAAGGTTTTGCC; ACTTTCATGGAGTATTTCTCTTTGGAAAAAATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATG; CTCGATTTAAAACCATGAAGAAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTTCAGTCTTC; AGAACAAATTGAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAA; ACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTTTACCTGCATAATCACAGCTATGCATCTA; TTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAAACCAAACACTAGATGTAAAGAGAATGAT; GAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACTGTTCAAAAGAAAGGAAAGGAACTTTTTATA; CAACAAGAGAGATTCTTTTTAAATATGAAGCCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATA; TTCCACTATCCTATGTCACTGAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTGGATAAGAA; ATCAGGTGTCATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATAAACATGAATGGTTATTAT; ATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCATCAGTTGAAAATAAATCCTTATGTTCTGA; GTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAACTTGCAGGCCTAGGCAAGGTACCTCTCAA; GAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAACCATACTGCACCCATCACCGAAGCCCTGTTT; CAGACAGACCTCATCTATAACCTCCTTGAAAAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTA; GGGTATTTAAATTACTTCAAAACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCATCTATGCG; AGAGCTTCGGTCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAACTGCTCTACTACTGCCATG; AAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGCCTACCTACTGATGTCATGACAACTGTGT; TCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTTTGGGCAAATACATTTCTATAGGCTCTGA; AGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCAGAGGATGTGCGGAAGCTTTACTGGTTAATG; AAAAGTAGCCTGAATGGAGATAACTTCCGAACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGAC; ATTTTCCTGGACACTTACTGGCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAGAAGTTCCC; TCTGGGGTCCTATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTTTCAACAAAGACACATTTA; TCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTCCAGGAGGCTT; TGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACCTCAAAAGTCTCACATGGTGGCTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220692 representing NM_005575; Red=Cloning site Green=Tags(s); ; MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDY; ESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVVAVSVIMVIYLLPRCTFTKEG; CHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSVTISVQALQVTWNIILH; STGHNISRVTFMSAVSSQEKQAEILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYT; DESNEKKYFAATQFEPLAARSAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSE; SVKMSTYLVAFIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLV; AIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFA; TFMEYFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLK; TYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQKGFPLVTVQKKGKELFI; QQERFFLNMKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYY; IVHYADDDWEALIHQLKINPYVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALF; QTDLIYNLLEKLGYMDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAM; KLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASSEDVRKLYWLM; KSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLFSTKTHL; SEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKSLTWWL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005575
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005575.3
• RefSeq Size: 4470 bp
• RefSeq ORF: 3078 bp
• Locus ID: 4012
• UniProt ID: Q9UIQ6
• Cytogenetics: 5q15
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system
• MW: 117.2 kDa
• Summary: This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]