H2AC21 (NM_175065) Human Tagged ORF Clone

  • TrueORF®
SKU
RC220685
HIST2H2AB (Myc-DDK-tagged)-Human histone cluster 2, H2ab (HIST2H2AB)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol H2AC21
Synonyms H2AB; HIST2H2AB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220685 representing NM_175065
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGACGCGGAAAGCAGGGAGGCAAGGCCCGCGCTAAGGCCAAGTCGCGCTCGTCCCGCGCTGGTC
TCCAGTTCCCGGTGGGGCGAGTGCACCGCTTGCTGCGCAAAGGCAACTACGCGGAGCGGGTCGGGGCAGG
CGCCCCGGTGTACCTGGCGGCGGTCCTCGAGTACCTGACCGCGGAAATTCTGGAGCTGGCGGGCAACGCG
GCTCGGGACAACAAGAAGACGCGCATCATCCCTCGCCATCTGCAACTAGCCGTGAGGAATGACGAAGAGC
TCAACAAGTTACTCGGGGGTGTCACCATTGCCCAGGGCGGCGTCTTGCCCAATATCCAGGCTGTCCTGTT
GCCCAAGAAAACGGAGAGTCACAAGCCTGGCAAGAACAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC220685 representing NM_175065
Red=Cloning site Green=Tags(s)

MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNA
ARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTESHKPGKNK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175065
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175065.3
RefSeq Size 448 bp
RefSeq ORF 393 bp
Locus ID 317772
UniProt ID Q8IUE6
Cytogenetics 1q21.2
Protein Pathways Systemic lupus erythematosus
MW 13.8 kDa
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_175065" in other vectors (4)
SKU Description Size Price
RC220685L3 Lenti ORF clone of Human histone cluster 2, H2ab (HIST2H2AB), Myc-DDK-tagged 10 ug
$450.00
RC220685L4 Lenti ORF clone of Human histone cluster 2, H2ab (HIST2H2AB), mGFP tagged 10 ug
$450.00
RG220685 HIST2H2AB (tGFP-tagged) - Human histone cluster 2, H2ab (HIST2H2AB) 10 ug
$350.00
SC306990 HIST2H2AB (untagged)-Human histone cluster 2, H2ab (HIST2H2AB) 10 ug
$165.00

Plasmid Map for RC220685

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Cloning scheme for RC220685

Circular map for RC220685

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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