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TSSK4 (NM_174944) Human Tagged ORF Clone

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SKU
RC220627
TSSK4 (Myc-DDK-tagged)-Human testis-specific serine kinase 4 (TSSK4), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TSSK4
Synonyms C14orf20; STK22E; TSK-4; TSK4; TSSK5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220627 representing NM_174944
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGGGAGATGTCTTAGAGGCAGCACCAACCACCACAGCCTACCATTCCCTCATGGATGAATATG
GTTATGAGGTGGGCAAGGCCATTGGCCATGGCTCCTATGGGTCGGTATATGAGGCTTTCTACACAAAGCA
GAAGGTTATGGTGGCAGTCAAGATCATCTCAAAGAAGAAGGCCTCTGATGACTATCTTAACAAGTTCCTG
CCCCGTGAAATACAGGTAATGAAAGTCTTGCGGCACAAGTACCTCATCAACTTCTATCGGGCCATTGAGA
GCACATCTCGAGTATACATCATTCTGGAACTGGCTCAGGGTGGTGATGTCCTTGAATGGATCCAGCGCTA
CGGGGCCTGCTCTGAGCCCCTTGCTGGCAAGTGGTTCTCCCAGCTGACCCTGGGCATTGCCTACCTGCAC
AGCAAGAGCATCGTGCACCGGGACTTAAAGTTGGAGAACCTGTTGCTGGACAAGTGGGAGAATGTGAAGA
TATCAGACTTTGGCTTTGCCAAGATGGTGCCTTCTAACCAGCCTGTGGGTTGTAGCCCTTCTTACCGCCA
AGTGAACTGCTTTTCCCACCTCAGCCAGACTTACTGTGGCAGCTTTGCTTACGCTTGCCCAGAGATCTTA
CGAGGCTTGCCCTACAACCCTTTCCTGTCTGACACCTGGAGCATGGGCGTCATCCTTTACACTCTAGTGG
TCGCCCATCTGCCCTTTGATGACACCAATCTCAAAAAGCTGCTAAGAGAGACTCAGAAGGAGGTCACTTT
CCCAGCTAACCATACCATCTCCCAGGAGTGCAAGAACCTGATCCTCCAGATGCTACGCCAAGCCACTAAG
CGTGCCACCATTCTGGACATCATCAAGGATTCCTGGGTGCTCAAGTTCCAGCCTGAGCAACCCACCCATG
AGATCAGGCTGCTTGAGGCCATGTGCCAGCTCCACAACACCACTAAACAGCACCAATCCTTGCAAATTAC
GACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220627 representing NM_174944
Red=Cloning site Green=Tags(s)

MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFYTKQKVMVAVKIISKKKASDDYLNKFL
PREIQVMKVLRHKYLINFYRAIESTSRVYIILELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLH
SKSIVHRDLKLENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPEIL
RGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQECKNLILQMLRQATK
RATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174944
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174944.4
RefSeq Size 1264 bp
RefSeq ORF 987 bp
Locus ID 283629
UniProt ID Q6SA08
Cytogenetics 14q12
Protein Families Druggable Genome, Protein Kinase
MW 37.3 kDa
Summary This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.provided by RefSeq, May 2010
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"NM_174944" in other vectors (6)
SKU Description Size Price
RC220627L1 Lenti ORF clone of Human testis-specific serine kinase 4 (TSSK4), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC220627L2 Lenti ORF clone of Human testis-specific serine kinase 4 (TSSK4), transcript variant 2, mGFP tagged 10 ug
$600.00
RC220627L3 Lenti ORF clone of Human testis-specific serine kinase 4 (TSSK4), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC220627L4 Lenti ORF clone of Human testis-specific serine kinase 4 (TSSK4), transcript variant 2, mGFP tagged 10 ug
$600.00
RG220627 TSSK4 (tGFP-tagged) - Human testis-specific serine kinase 4 (TSSK4), transcript variant 2 10 ug
$500.00
SC101301 TSSK4 (untagged)-Human testis-specific serine kinase 4 (TSSK4), transcript variant 2 10 ug
$300.00

Plasmid Map for RC220627

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220627
Circular map for RC220627

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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