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TTYH1 (NM_020659) Human Tagged ORF Clone

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SKU
RC220380
TTYH1 (Myc-DDK-tagged)-Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TTYH1 or NM_020659"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTYH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220380 representing NM_020659
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGCCCCCGGGCTACCGGCCCTCAGCTTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGACT
TCCAGCTCCGCCCGGTGCCCAGCGTTTTCGCGCCCCAAGAGCAGGAATACCAGCAGGCCTTGTTGCTGGT
GGCGGCCTTGGCGGGCCTGGGCTTGGGCCTGAGCCTCATTTTCATCGCTGTCTACCTCATCCGCTTCTGC
TGCTGCCGGCCCCCCGAGCCCCCCGGGTCCAAGATCCCCTCGCCCGGGGGAGGCTGCGTCACCTGGAGCT
GCATTGTCGCCCTTCTCGCCGGCTGCACTGGCATTGGCATCGGTTTCTATGGCAACAGTGAGACCAGTGA
TGGGGTGTCCCAGCTCAGCTCTGCGCTGCTGCACGCCAACCACACACTCAGCACCATTGACCACCTGGTG
TTGGAGACGGTGGAGAGGCTGGGCGAGGCGGTGAGGACAGAGCTGACCACCCTGGAGGAGGTGCTCGAGC
CGCGCACGGAGCTGGTGGCTGCCGCCCGAGGGGCTCGACGGCAGGCGGAGGCTGCGGCCCAGCAGCTGCA
GGGGCTGGCCTTCTGGCAGGGAGTGCCCCTGAGCCCCCTGCAGGTGGCTGAAAATGTGTCCTTTGTGGAG
GAGTACAGGTGGCTGGCCTACGTCCTCCTGCTGCTCCTGGAGCTGCTGGTCTGCCTCTTCACCCTCCTGG
GCCTGGCGAAGCAGAGCAAGTGGCTGGTGATCGTGATGACAGTCATGAGTCTCCTGGTTCTCGTCCTGAG
CTGGGGCTCCATGGGCCTGGAGGCAGCCACGGCCGTGGGCCTCAGTGACTTCTGCTCCAATCCAGACCCT
TATGTTCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTATTATCTCCTCTGCA
ACCGGGCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCTGGCCAACATCCACTC
CCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTCAGCGCAGAAGCCTCTGCTGTCCTTG
GAGGAGACTCTGAATGTGACAGAAGGAAATTTCCACCAGTTGGTGGCACTGCTACACTGCCGCAGCCTGC
ACAAGGACTATGGTGCAGCCCTGCGGGGCCTGTGCGAAGACGCCCTGGAAGGCCTGCTCTTCCTGCTACT
CTTCTCCCTGCTGTCTGCAGGAGCGCTGGCCACTGCCCTCTGCAGCCTGCCCCGAGCCTGGGCCCTCTTC
CCACCCAGTGACGACTACGATGACACAGACGATGACGACCCTTTCAACCCTCAGGAATCCAAGCGCTTTG
TGCAGTGGCAGTCGTCTATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220380 representing NM_020659
Red=Cloning site Green=Tags(s)

MGAPPGYRPSAWVHLLHQLPRADFQLRPVPSVFAPQEQEYQQALLLVAALAGLGLGLSLIFIAVYLIRFC
CCRPPEPPGSKIPSPGGGCVTWSCIVALLAGCTGIGIGFYGNSETSDGVSQLSSALLHANHTLSTIDHLV
LETVERLGEAVRTELTTLEEVLEPRTELVAAARGARRQAEAAAQQLQGLAFWQGVPLSPLQVAENVSFVE
EYRWLAYVLLLLLELLVCLFTLLGLAKQSKWLVIVMTVMSLLVLVLSWGSMGLEAATAVGLSDFCSNPDP
YVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSL
EETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALEGLLFLLLFSLLSAGALATALCSLPRAWALF
PPSDDYDDTDDDDPFNPQESKRFVQWQSSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020659
ORF Size 1350 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020659.4
RefSeq Size 2045 bp
RefSeq ORF 1353 bp
Locus ID 57348
UniProt ID Q9H313
Cytogenetics 19q13.42
Protein Families Ion Channels: Other, Transmembrane
MW 48.9 kDa
Summary This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent, volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jan 2011
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Documents
Resources
"NM_020659" in other vectors (4)
SKU Description Size Price
RC220380L3 Lenti ORF clone of Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC220380L4 Lenti ORF clone of Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG220380 TTYH1 (tGFP-tagged) - Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1 10 ug
$657.00
SC310371 TTYH1 (untagged)-Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1 10 ug
$503.00

Plasmid Map for RC220380

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220380
Circular map for RC220380

file-alt Citations

Delimitation of the Pore in Tweety Homolog 1 Channels: A Model-guided Approach
Reyes, Hernández, Cervantes et al
Journal of Advances in Biology & Biotechnology (2016) 10 (1), 1
Snippet: 2. MATERIALS AND METHODS 2.1 Clones, Cells and Transfection The hTTYH1 clone was purchased from Origene (Rockville, MD, USA), and sequenced at Biotechnology Facility of the National Autonomous University of Mexico, UNAM. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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