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LRIT2 (NM_001017924) Human Tagged ORF Clone

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SKU
RC220290
LRIT2 (Myc-DDK-tagged)-Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 (LRIT2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $512.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRIT2
Synonyms LRRC22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220290 representing NM_001017924
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAGTTTTTCATTACTTCCTGTTAGTTCTGGTCTTTCTGGATACACACGCAGCTCAGCCTTTCT
GTCTGCCAGGATGCACTTGCTCAGAGGAGAGTTTTGGCAGGACTCTGCAGTGCACATCTGTCTCCTTGGG
AAAGATCCCTGGGAACCTTTCTGAAGAGTTCAAGCAAGTGAGAATTGAAAATTCACCCTTATTTGAGATG
CCCCAAGGGTCTTTCATCAACATGAGCACCTTGGAATACCTCTGGCTCAATTTTAACAATATCAGTGTGA
TCCACCTAGGAGCCCTGGAACACCTGCCAGAACTGAGGGAGCTGAGACTGGAGGGGAACAAGCTCTGCTC
AGTACCATGGACAGCGTTCCGTGCCACCCCTCTCCTGAGGGTCTTGGATCTCAAACGCAACAAGATTGAT
GCACTCCCTGAGCTGGCTCTTCAATTCTTGGTCAGCCTGACCTACCTTGACCTATCCTCCAATAGGCTTA
CAGTTGTATCCAAGAGTGTCTTCCTGAACTGGCCAGCCTACCAGAAATGCCGGCAGCCTGACTGTGGGGC
TGAGATTCTCTCCAGCCTGGTGGTGGCCCTGCATGACAACCCCTGGGTATGTGACTGTCGCCTAAGGGGG
CTTGTCCAGTTTGTCAAGTCCATTACCCTCCCAGTCATCCTGGTGAATTCCTACCTGATATGTCAGGGCC
CTCTGTCCAAGGCAGGGCAGCTTTTTCATGAAACTGAGCTTAGTGCTTGCATGAAGCCACAGATCTCAAC
CCCCAGTGCCAATATCACCATCCGGGCAGGACAGAATGTGACCCTGCGATGCTTGGCACAGGCCAGCCCC
TCACCATCCATTGCATGGACTTATCCCCTGAGTATGTGGAGAGAATTTGATGTGTTGACATCTTCTACTG
GAGAAGACACTGCTCTGTCAGAGCTGGCCATACCTGCTGCCCACCTGGTAGACAGTGGTAATTACACCTG
CATGGCCTCCAACTCCATTGGCAAGAGCAACCTTGTAATCTCTCTCCATGTCCAGCCTGCCCAGGCCCTA
CATGCACCTGATTCTCTTTCCATCCCCTCGGAGGGCAATGCCTACATTGACCTGCGGGTTGTCAAGCAGA
CAGTGCATGGGATTTTGCTGGAGTGGCTTGCAGTGGCTGACACCTCTAAGGAGGAGTGGTTCACCCTCTA
CATTGCATCGGATGAAGCCTTCAGGAAGGAGGTGGTTCACATTGGCCCCGGAATCAATACTTATGCTGTG
GATGACCTCCTTCCTGGCACAAAATATGAGGCCTGCCTCAGCCTAGAGGGCCAGCCTCCACACCAGGGCC
AGTGTGTAGCTTTTGTAACAGGCAGAGATGCTGGTGGGCTAGAGGCACGTGAGCACCTCCTGCATGTCAC
AGTGGTCCTGTGTGTGGTGCTGCTTGCAGTGCCTGTGGGCGCCTATGCCTGGGCAGCCCAGGGCCCCTGC
AGCTGCAGCAAGTGGGTCCTGCGCGGCTGTCTTCATCGCAGGAAAGCCCCCAGCTGCACCCCTGCAGCCC
CGCAGTCCAAGGATGGCTCCTTTAGAGAACATCCAGCTGTCTGTGATGACGGTGAAGGGCACATAGACAC
TGAGGGGGACAAGGAGAAAGGAGGAACGGAAGACAACAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220290 representing NM_001017924
Red=Cloning site Green=Tags(s)

MASVFHYFLLVLVFLDTHAAQPFCLPGCTCSEESFGRTLQCTSVSLGKIPGNLSEEFKQVRIENSPLFEM
PQGSFINMSTLEYLWLNFNNISVIHLGALEHLPELRELRLEGNKLCSVPWTAFRATPLLRVLDLKRNKID
ALPELALQFLVSLTYLDLSSNRLTVVSKSVFLNWPAYQKCRQPDCGAEILSSLVVALHDNPWVCDCRLRG
LVQFVKSITLPVILVNSYLICQGPLSKAGQLFHETELSACMKPQISTPSANITIRAGQNVTLRCLAQASP
SPSIAWTYPLSMWREFDVLTSSTGEDTALSELAIPAAHLVDSGNYTCMASNSIGKSNLVISLHVQPAQAL
HAPDSLSIPSEGNAYIDLRVVKQTVHGILLEWLAVADTSKEEWFTLYIASDEAFRKEVVHIGPGINTYAV
DDLLPGTKYEACLSLEGQPPHQGQCVAFVTGRDAGGLEAREHLLHVTVVLCVVLLAVPVGAYAWAAQGPC
SCSKWVLRGCLHRRKAPSCTPAAPQSKDGSFREHPAVCDDGEGHIDTEGDKEKGGTEDNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001017924
ORF Size 1650 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001017924.5
RefSeq Size 3221 bp
RefSeq ORF 1653 bp
Locus ID 340745
UniProt ID A6NDA9
Cytogenetics 10q23.1
Protein Families Transmembrane
MW 60 kDa
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Documents
Resources
"NM_001017924" in other vectors (4)
SKU Description Size Price
RC220290L3 Lenti ORF clone of Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 (LRIT2), Myc-DDK-tagged 10 ug
$812.00
RC220290L4 Lenti ORF clone of Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 (LRIT2), mGFP tagged 10 ug
$812.00
RG220290 LRIT2 (tGFP-tagged) - Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 (LRIT2) 10 ug
$712.00
SC302077 LRIT2 (untagged)-Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 (LRIT2) 10 ug
$564.00

Plasmid Map for RC220290

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220290
Circular map for RC220290

file-alt Citations

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