ADCY3 (NM_004036) Human Tagged ORF Clone

SKU RC220272

ADCY3 (Myc-DDK-tagged)-Human adenylate cyclase 3 (ADCY3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ADCY3
• Synonyms: AC-III; AC3; BMIQ19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220272 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCGAGGAACCAGGGCTTCTCCGAGCCCGAATACTCGGCCGAGTACTCAGCCGAGTACTCCGTCAGCC; TGCCCTCCGACCCTGACCGCGGGGTGGGCCGGACCCATGAAATCTCGGTCCGGAACTCGGGCTCCTGCCT; GTGCCTGCCTCGCTTCATGCGGCTGACTTTCGTGCCGGAGTCCTTGGAGAACCTCTACCAGACCTACTTC; AAAAGGCAGCGCCACGAGACCCTGCTGGTGCTGGTGGTCTTTGCAGCCCTCTTTGACTGCTACGTGGTGG; TCATGTGTGCTGTGGTCTTCTCCAGCGACAAGCTGGCTTCCCTCGCCGTGGCTGGAATTGGACTGGTGTT; GGACATCATCCTCTTCGTGCTCTGCAAAAAGGGGCTGCTCCCGGACCGGGTCACCCGCAGAGTGCTGCCC; TACGTGCTGTGGCTGCTCATAACCGCCCAGATCTTCTCCTACCTGGGCCTGAACTTCGCGCGTGCCCACG; CGGCTAGTGACACGGTGGGCTGGCAGGTCTTCTTTGTCTTCTCCTTCTTCATCACGCTGCCCCTCAGCCT; CAGCCCCATCGTGATCATCTCCGTGGTCTCCTGTGTGGTGCACACGTTGGTCCTGGGGGTCACCGTGGCC; CAGCAGCAGCAGGAGGAGCTCAAGGGGATGCAGCTGCTGCGGGAGATCCTGGCCAACGTCTTCCTCTACC; TGTGCGCCATCGCTGTGGGCATCATGTCCTACTACATGGCTGACCGCAAGCACCGCAAGGCCTTCCTGGA; GGCCCGCCAGTCGCTGGAGGTGAAGATGAACCTGGAAGAGCAGAGCCAGCAGCAGGAGAACCTCATGCTT; TCCATCCTGCCCAAGCACGTGGCTGACGAGATGCTGAAAGACATGAAGAAAGACGAGAGCCAGAAGGACC; AGCAGCAGTTCAACACCATGTACATGTACCGTCACGAGAACGTCAGCATCCTCTTTGCCGACATCGTGGG; CTTTACCCAGCTGTCTTCTGCCTGCAGTGCCCAGGAGCTTGTGAAGCTGCTCAACGAGCTCTTTGCCCGC; TTTGACAAGCTGGCAGCTAAATACCACCAGCTGCGGATTAAGATCCTGGGCGACTGCTACTACTGCATCT; GCGGCTTGCCCGACTACCGGGAGGACCACGCCGTCTGCTCCATCCTCATGGGGCTGGCCATGGTGGAGGC; CATCTCGTATGTGCGGGAGAAGACCAAGACTGGGGTGGACATGCGTGTGGGGGTGCACACGGGCACCGTG; CTGGGGGGCGTCCTGGGCCAGAAGCGCTGGCAGTACGACGTGTGGTCGACTGATGTCACTGTAGCCAACA; AGATGGAGGCCGGCGGCATCCCTGGGCGCGTGCACATCTCCCAGAGCACCATGGACTGCCTGAAAGGGGA; GTTTGATGTGGAGCCAGGCGATGGGGGCAGCCGCTGTGATTACCTAGAAGAGAAGGGTATTGAAACCTAC; CTCATCATTGCCTCCAAGCCAGAGGTGAAGAAAACAGCCACCCAGAATGGCCTCAATGGCTCGGCCCTGC; CCAATGGAGCACCAGCTTCCTCAAAGTCCAGCTCCCCTGCCCTCATTGAGACCAAGGAGCCCAACGGGAG; TGCCCACAGCAGTGGGTCCACGTCGGAGAAGCCCGAGGAGCAGGATGCCCAGGCCGACAACCCCTCATTC; CCCAACCCACGCCGGAGGCTGCGCCTGCAGGACCTGGCTGACCGAGTGGTGGATGCCTCTGAAGATGAGC; ACGAGCTCAACCAGCTGCTCAACGAGGCCCTGCTTGAGCGAGAGTCCGCCCAAGTAGTAAAGAAGAGAAA; CACCTTCCTCTTGTCCATGCGGTTCATGGACCCCGAGATGGAAACCCGCTACTCGGTGGAGAAGGAGAAG; CAGAGTGGGGCTGCCTTCAGCTGCTCCTGCGTCGTCCTGCTCTGCACGGCCCTGGTCGAGATACTCATCG; ACCCCTGGCTAATGACAAACTATGTGACCTTCATGGTGGGGGAGATTCTGCTCCTCATCCTGACCATCTG; CTCCCTGGCTGCCATCTTTCCCCGGGCCTTTCCTAAGAAGCTTGTGGCCTTCTCAACTTGGATTGACCGG; ACCCGCTGGGCCAGGAACACCTGGGCCATGCTCGCCATCTTCATCCTGGTGATGGCAAATGTCGTGGACA; TGCTCAGCTGTCTCCAGTACTACACGGGACCCAGCAATGCAACGGCAGGGATGGAAACGGAGGGCAGCTG; CCTGGAGAACCCCAAGTATTACAACTATGTGGCCGTGCTGTCCCTCATCGCCACCATCATGCTGGTGCAG; GTCAGCCACATGGTGAAGCTCACGCTCATGCTGCTCGTCGCAGGCGCCGTGGCCACCATCAACCTCTATG; CCTGGCGTCCCGTCTTTGATGAATACGACCACAAGCGTTTTCGGGAGCACGACTTACCTATGGTGGCCTT; AGAGCAGATGCAAGGATTCAACCCTGGGCTCAATGGCACTGACAGGCTGCCCCTGGTGCCTTCCAAGTAC; TCTATGACGGTGATGGTGTTCCTCATGATGCTCAGCTTCTACTACTTCTCCCGCCACGTAGAAAAACTGG; CACGGACACTTTTCTTGTGGAAGATTGAGGTCCACGACCAGAAGGAACGTGTCTATGAGATGCGACGCTG; GAACGAGGCCTTGGTCACCAACATGTTGCCTGAGCACGTGGCACGCCATTTCCTGGGGTCCAAGAAGAGA; GATGAGGAGCTGTATAGCCAGACGTATGATGAGATTGGAGTCATGTTTGCCTCCCTGCCCAACTTTGCTG; ACTTCTACACAGAGGAGAGCATCAACAATGGTGGTATTGAGTGTCTGCGTTTCCTCAATGAAATCATCTC; AGATTTTGACTCTCTCCTGGACAATCCCAAGTTCCGGGTGATCACCAAGATCAAAACCATTGGCAGCACG; TATATGGCGGCTTCAGGAGTCACCCCCGATGTCAACACCAATGGCTTTGCCAGCTCCAACAAGGAAGACA; AGTCCGAGAGAGAGCGCTGGCAGCACCTGGCTGACCTGGCCGACTTCGCGCTGGCCATGAAGGATACGCT; CACCAACATCAACAACCAGTCCTTCAATAACTTCATGCTGCGCATAGGCATGAACAAAGGCGGGGTTCTG; GCTGGGGTCATCGGAGCCCGGAAACCACACTACGACATCTGGGGCAATACAGTCAATGTAGCCAGCAGGA; TGGAGTCCACGGGGGTCATGGGCAACATTCAGGTGGTAGAAGAAACCCAAGTCATCCTCCGAGAGTACGG; CTTCCGCTTTGTGAGGCGAGGCCCCATCTTTGTGAAGGGGAAGGGGGAGCTGCTGACCTTCTTCTTGAAG; GGGCGGGATAAGCTAGCCACCTTCCCCAATGGCCCCTCTGTCACACTGCCCCACCAGGTGGTGGACAACT; CC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220272 protein sequence; Red=Cloning site Green=Tags(s); ; MPRNQGFSEPEYSAEYSAEYSVSLPSDPDRGVGRTHEISVRNSGSCLCLPRFMRLTFVPESLENLYQTYF; KRQRHETLLVLVVFAALFDCYVVVMCAVVFSSDKLASLAVAGIGLVLDIILFVLCKKGLLPDRVTRRVLP; YVLWLLITAQIFSYLGLNFARAHAASDTVGWQVFFVFSFFITLPLSLSPIVIISVVSCVVHTLVLGVTVA; QQQQEELKGMQLLREILANVFLYLCAIAVGIMSYYMADRKHRKAFLEARQSLEVKMNLEEQSQQQENLML; SILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFAR; FDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTV; LGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVEPGDGGSRCDYLEEKGIETY; LIIASKPEVKKTATQNGLNGSALPNGAPASSKSSSPALIETKEPNGSAHSSGSTSEKPEEQDAQADNPSF; PNPRRRLRLQDLADRVVDASEDEHELNQLLNEALLERESAQVVKKRNTFLLSMRFMDPEMETRYSVEKEK; QSGAAFSCSCVVLLCTALVEILIDPWLMTNYVTFMVGEILLLILTICSLAAIFPRAFPKKLVAFSTWIDR; TRWARNTWAMLAIFILVMANVVDMLSCLQYYTGPSNATAGMETEGSCLENPKYYNYVAVLSLIATIMLVQ; VSHMVKLTLMLLVAGAVATINLYAWRPVFDEYDHKRFREHDLPMVALEQMQGFNPGLNGTDRLPLVPSKY; SMTVMVFLMMLSFYYFSRHVEKLARTLFLWKIEVHDQKERVYEMRRWNEALVTNMLPEHVARHFLGSKKR; DEELYSQTYDEIGVMFASLPNFADFYTEESINNGGIECLRFLNEIISDFDSLLDNPKFRVITKIKTIGST; YMAASGVTPDVNTNGFASSNKEDKSERERWQHLADLADFALAMKDTLTNINNQSFNNFMLRIGMNKGGVL; AGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEETQVILREYGFRFVRRGPIFVKGKGELLTFFLK; GRDKLATFPNGPSVTLPHQVVDNS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004036
• ORF Size: 3432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004036.5
• RefSeq Size: 4410 bp
• RefSeq ORF: 3435 bp
• Locus ID: 109
• UniProt ID: O60266
• Cytogenetics: 2p23.3
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction, Vibrio cholerae infection
• MW: 129 kDa
• Summary: This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]