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INS-IGF2 (NM_001042376) Human Tagged ORF Clone

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SKU
RC220218
INS (Myc-DDK-tagged)-Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol INS-IGF2
Synonyms INSIGF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220218 representing NM_001042376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAG
CCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGG
CTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGCCTCAGCTTTGTCCCTCTCCTCC
TCCACGTCAACCTGGCCAGAGGGTCTGGACGCCACAGCCAGGGCACCCCCTGCTTTGGTGGTGACTGCTA
ATATTGGCCAGGCCGGCGGATCATCGTCCAGGCAGTTTCGGCAGAGAGCCTTGGGCACCAGTGACTCCCC
GGTCCTCTTTATCCACTGTCCAGGAGCTGCGGGGACTGCGCAGGGACTAGAGTACAGGGGCCGAAGAGTC
ACCACCGAGCTTGTGTGGGAGGAGGTGGATTCCAGCCCCCAGCCCCAGGGCTCTGAATCGCTGCCAGCTC
AGCCCCCTGCCCAGCCTGCCCCACAGCCTGAGCCCCAGCAGGCCAGAGAGCCCAGTCCTGAGGTGAGCTG
CTGTGGCCTGTGGCCCAGGCGACCCCAGCGCTCCCAGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220218 representing NM_001042376
Red=Cloning site Green=Tags(s)

MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQASALSLSS
STSTWPEGLDATARAPPALVVTANIGQAGGSSSRQFRQRALGTSDSPVLFIHCPGAAGTAQGLEYRGRRV
TTELVWEEVDSSPQPQGSESLPAQPPAQPAPQPEPQQAREPSPEVSCCGLWPRRPQRSQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042376
ORF Size 600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042376.3
RefSeq Size 828 bp
RefSeq ORF 603 bp
Locus ID 723961
UniProt ID F8WCM5
Cytogenetics 11p15.5
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
MW 21.4 kDa
Summary This locus includes two alternatively spliced read-through transcript variants which align to the INS gene in the 5' region and to the IGF2 gene in the 3' region. One transcript is predicted to encode a protein which shares the N-terminus with the INS protein but has a distinct and longer C-terminus, whereas the other transcript is a candidate for nonsense-mediated decay (NMD). The transcripts are imprinted and are paternally expressed in the limb and eye. provided by RefSeq, Jul 2008
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"NM_001042376" in other vectors (4)
SKU Description Size Price
RC220218L3 Lenti ORF clone of Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC220218L4 Lenti ORF clone of Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mGFP tagged 10 ug
$600.00
RG220218 INS (tGFP-tagged) - Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2 10 ug
$500.00
SC311232 INS (untagged)-Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2 10 ug
$330.00

Plasmid Map for RC220218

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220218
Circular map for RC220218

file-alt Citations

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