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ARHGEF38 (NM_017700) Human Tagged ORF Clone

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SKU
RC220086
ARHGEF38 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 38 (ARHGEF38)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARHGEF38
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220086 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCAAAGAAGCCACTGGGAAAGAAAACATGGTCACCAAGAAAAAGAATCTGGCCTTCTTGAGGT
CTAGACTCTATATGCTGGAGAGAAGGAAGACTGACACTGTGGTTGAGAGCAGTGTTTCTGGGGACCACTC
TGGCACCTTGAGGAGGAGCCAATCTGACAGGACCGAATACAACCAGAAATTACAAGAAAAGATGACTCCA
CAGGGTGAGTGTTCTGTAGCTGAGACCTTAACCCCAGAGGAAGAGCATCATATGAAGAGGATGATGGCAA
AGCGGGAAAAGATCATTAAGGAGCTGATACAGACAGAAAAGGATTATCTCAATGATCTAGAGCTGTGTGT
TAGGGAAGTGGTTCAGCCCCTGAGAAATAAAAAGACTGATAGGCTGGATGTGGATAGCTTGTTTAGCAAC
ATTGAGTCCGTGCATCAGATATCAGCCAAGCTGCTGTCATTGTTGGAAGAGGCCACAACAGACGTGGAAC
CGGCCATGCAAGTAATTGGAGAAGTATTCTTGCAGATTAAAGGGCCACTGGAAGATATTTATAAAATCTA
CTGCTATCACCATGATGAAGCACATAGTATACTGGAGTCCTATGAAAAGGAAGAAGAGCTGAAGGAACAT
TTGAGCCACTGTATCCAGTCCTTAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220086 protein sequence
Red=Cloning site Green=Tags(s)

MEPKEATGKENMVTKKKNLAFLRSRLYMLERRKTDTVVESSVSGDHSGTLRRSQSDRTEYNQKLQEKMTP
QGECSVAETLTPEEEHHMKRMMAKREKIIKELIQTEKDYLNDLELCVREVVQPLRNKKTDRLDVDSLFSN
IESVHQISAKLLSLLEEATTDVEPAMQVIGEVFLQIKGPLEDIYKIYCYHHDEAHSILESYEKEEELKEH
LSHCIQSLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017700
ORF Size 657 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017700.1, NP_060170.1
RefSeq Size 1453 bp
RefSeq ORF 660 bp
Locus ID 54848
UniProt ID Q9NXL2
Cytogenetics 4q24
MW 25.5 kDa
Summary May act as a guanine-nucleotide releasing factor.UniProtKB/Swiss-Prot Function
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"NM_017700" in other vectors (4)
SKU Description Size Price
RC220086L3 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 38 (ARHGEF38), Myc-DDK-tagged 10 ug
$600.00
RC220086L4 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 38 (ARHGEF38), mGFP tagged 10 ug
$600.00
RG220086 ARHGEF38 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 38 (ARHGEF38) 10 ug
$489.00 $500.00
SC108652 ARHGEF38 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 38 (ARHGEF38) 10 ug
$300.00

Plasmid Map for RC220086

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220086
Circular map for RC220086

file-alt Citations

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