ALPK3 (NM_020778) Human Tagged ORF Clone
$2,403.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ALPK3 |
| Synonyms | CMH27; MAK; MIDORI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020778 |
| ORF Size | 5721 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020778.2, NP_065829.2 |
| RefSeq Size | 10914 bp |
| RefSeq ORF | 5118 bp |
| Locus ID | 57538 |
| UniProt ID | Q96L96 |
| Cytogenetics | 15q25.3 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 201.1 kDa |
| Summary | Involved in cardiomyocyte differentiation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020778" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220076L1 | Lenti ORF clone of Human alpha-kinase 3 (ALPK3), Myc-DDK-tagged | 10 ug |
$2,703.00
|
|
| RG220076 | ALPK3 (tGFP-tagged) - Human alpha-kinase 3 (ALPK3) | 10 ug |
$2,603.00
|
|
| SC309405 | ALPK3 (untagged)-Human alpha-kinase 3 (ALPK3) | 10 ug |
$2,565.00
|
|
Citations
Pathogenesis of Cardiomyopathy Caused by Variants in ALPK3, an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere
Agarwal, Wakimoto, Paulo et al
Circulation (2022) 146 (22), 1674-1693 DOI: 10.1161/CIRCULATIONAHA.122.059688
Agarwal, Wakimoto, Paulo et al
Circulation (2022) 146 (22), 1674-1693 DOI: 10.1161/CIRCULATIONAHA.122.059688
AI Summary: OriGene’s human ALPK3-FLAG plasmid (OriGene RC220076) was used to transiently overexpress ALPK3 in HEK293T cells, enabling the study of ALPK3’s molecular function and its role in cardiomyopathy pathogenesis.
Snippet: rack software.24 Transient Transfection of HEK293T Cells HEK293T cells were grown in 100-mm culture dishes to 80% confluency and transfected with 7.5 μg total plasmid DNA encoding human ALPK3-FLAG (Origene RC220076) using Lipofectamine 3000 (Invitrogen L3000001) per the manufacturer’s protocol alongside nontransfected control dishes. Cells were harvested at 72 hours after transfection for anal
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