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PTPD1 (PTPN21) (NM_007039) Human Tagged ORF Clone

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SKU
RC220032
PTPN21 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,022.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTPD1
Synonyms PTPD1; PTPRL10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220032 representing NM_007039
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACTGCCATTTGGGTTGAAACTGAAACGCACCCGGCGCTACACGGTGTCCAGCAAGAGTTGCCTGG
TTGCCCGGATCCAACTGCTTAATAACGAGTTTGTGGAGTTCACCCTGTCCGTGGAGAGCACTGGCCAGGA
AAGCCTCGAGGCCGTGGCCCAGAGGCTGGAGCTGCGGGAGGTCACTTACTTCAGCCTCTGGTACTACAAC
AAGCAAAATCAGCGCCGGTGGGTAGATTTGGAAAAACCTTTGAAGAAGCAGCTGGATAAATATGCATTGG
AACCTACCGTCTATTTTGGAGTGGTGTTTTATGTGCCTTCAGTTTCTCAGCTGCAGCAGGAGATTACCAG
GTATCAGTATTATCTGCAACTGAAGAAAGATATCTTGGAAGGAAGTATTCCTTGTACCTTAGAACAAGCA
ATTCAGCTAGCAGGCTTAGCTGTTCAAGCGGATTTTGGTGACTTTGATCAGTATGAATCCCAGGACTTTC
TTCAGAAATTTGCCTTGTTTCCTGTGGGATGGTTACAAGATGAAAAAGTATTGGAAGAAGCAACCCAAAA
AGTGGCCTTACTACATCAGAAATACAGAGGGCTCACAGCTCCTGATGCTGAAATGCTGTACATGCAGGAG
GTAGAGAGAATGGATGGCTATGGAGAAGAGAGCTACCCTGCTAAGGATAGCCAAGGAAGTGACATATCCA
TTGGAGCGTGTCTTGAAGGTATCTTTGTGAAACACAAGAATGGAAGGCATCCTGTGGTATTTAGGTGGCA
TGACATTGCCAACATGTCCCACAACAAGTCCTTTTTTGCATTAGAGCTGGCAAATAAAGAGGAGACCATT
CAATTTCAAACTGAAGACATGGAAACAGCAAAATACATTTGGAGACTCTGTGTTGCGCGACACAAGTTTT
ACAGACTAAACCAGTGTAACCTGCAAACTCAGACTGTCACAGTGAACCCAATCAGGAGGAGGTCTTCTTC
AAGGATGTCTCTGCCTAAACCCCAGCCCTACGTGATGCCTCCCCCACCGCAGTTGCACTATAATGGACAT
TATACAGAACCATATGCTTCTTCCCAAGATAACCTCTTTGTGCCCAACCAGAACGGATACTACTGTCACT
CTCAGACAAGCTTGGATAGAGCCCAGATTGACCTCAACGGTCGGATCCGTAATGGCAGTGTCTACAGTGC
ACACAGCACCAACTCCTTAAATAATCCTCAGCCCTACTTGCAGCCCTCGCCGATGTCGTCCAACCCTAGC
ATCACCGGGAGTGACGTCATGAGGCCTGACTACCTCCCGTCCCATCGGCACAGCGCCGTGATACCCCCGT
CCTACCGCCCCACCCCAGACTATGAGACTGTGATGAAGCAGCTCAACAGGGGCCTGGTGCATGCGGAACG
GCAGAGCCACTCGCTGCGAAACCTCAACATCGGCAGCTCGTACGCCTACAGCAGGCCCGCGGCGCTGGTC
TACAGCCAGCCCGAGATCCGCGAGCACGCACAGCTCCCCTCGCCAGCGGCCGCACACTGCCCGTTCAGCC
TGAGCTACAGCTTCCACAGCCCGTCTCCCTACCCCTACCCTGCCGAGCGGCGGCCCGTGGTGGGCGCGGT
CAGCGTGCCGGAGCTGACCAATGCGCAGCTGCAGGCGCAGGACTACCCGTCTCCCAACATCATGCGGACG
CAGGTGTACCGGCCACCCCCACCCTACCCGCCCCCCAGGCCCGCCAACAGCACGCCAGACCTGTCCCGCC
ACCTTTACATCAGCAGCAGCAACCCCGACCTCATCACGCGGCGCGTGCACCACTCGGTGCAAACGTTCCA
GGAGGACAGCCTGCCCGTGGCGCACTCGCTGCAGGAGGTCAGCGAGCCCCTCACCGCCGCGCGCCACGCG
CAGCTGCACAAACGGAACAGCATCGAGGTGGCCGGGCTCAGCCACGGCCTGGAGGGCCTGCGGCTCAAGG
AGCGCACCCTATCCGCGTCGGCGGCAGAGGTGGCGCCGCGAGCCGTCTCGGTGGGCTCCCAGCCCAGCGT
TTTCACCGAGAGGACACAGCGAGAAGGGCCGGAGGAGGCGGAGGGCTTGAGGTACGGCCATAAGAAGTCC
CTGTCGGACGCCACCATGCTAATCCACAGCAGCGAGGAGGAGGAGGACGAGGACTTCGAGGAGGAGAGCG
GGGCCCGGGCGCCTCCTGCACGTGCGCGCGAGCCTCGGCCCGGCCTGGCCCAGGACCCACCTGGCTGCCC
TCGCGTCCTGCTCGCCGGGCCCCTGCACATCCTGGAGCCCAAGGCCCACGTCCCAGACGCGGAGAAGAGG
ATGATGGACAGCAGCCCCGTCCGCACGACCGCAGAGGCCCAGCGGCCCTGGAGAGACGGGCTGCTGATGC
CCTCCATGTCGGAGTCCGACCTCACCACGTCAGGCCGCTACCGAGCCCGGAGGGACTCTCTGAAGAAAAG
GCCGGTGTCGGACCTTCTCTCTGGGAAGAAGAACATCGTGGAAGGGCTCCCGCCTCTAGGGGGAATGAAA
AAGACTCGAGTAGATGCAAAAAAAATTGGTCCTCTTAAACTGGCTGCCCTAAATGGACTCTCCCTATCTC
GAGTGCCTCTGCCTGATGAAGGAAAGGAAGTGGCTACCAGAGCAACGAATGATGAAAGGTGTAAAATTCT
GGAACAACGATTAGAACAAGGAATGGTATTCACAGAATATGAAAGAATTCTTAAGAAACGGCTAGTTGAT
GGGGAGTGCTCAACAGCACGACTCCCTGAAAATGCAGAAAGAAATCGATTCCAAGATGTTCTTCCTTATG
ATGATGTGAGAGTGGAGTTGGTCCCAACTAAAGAAAACAACACTGGTTACATCAACGCATCACATATTAA
GGTCTCTGTCAGTGGAATCGAATGGGATTATATTGCCACACAGGGACCATTACAGAATACCTGTCAAGAT
TTTTGGCAGATGGTATGGGAACAGGGAATTGCAATTATAGCAATGGTGACAGCAGAAGAGGAGGGTGGAA
GGGAGAAGAGCTTTAGGTACTGGCCACGACTTGGTTCCAGGCACAACACTGTCACCTATGGAAGGTTTAA
GATCACGACCCGGTTCCGCACAGACTCTGGCTGCTATGCCACCACAGGCCTGAAGATGAAGCACCTCCTT
ACTGGGCAAGAGAGGACCGTCTGGCACCTCCAATACACAGACTGGCCTGAACATGGCTGTCCAGAAGACC
TCAAGGGATTTTTATCATATCTTGAAGAGATCCAGTCTGTTCGACGCCATACAAATAGCACAAGTGATCC
CCAAAGCCCCAACCCTCCGTTGTTGGTCCACTGCAGTGCTGGGGTAGGAAGGACTGGCGTGGTGATTTTG
TCGGAGATCATGATCGCCTGCCTGGAACACAATGAGGTGCTGGACATCCCGAGAGTGCTGGACATGCTGA
GGCAACAGAGAATGATGCTGGTGCAGACTCTCTGCCAGTACACATTTGTGTACAGAGTCCTCATCCAGTT
CCTGAAAAGCTCCAGGCTCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220032 representing NM_007039
Red=Cloning site Green=Tags(s)

MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELREVTYFSLWYYN
KQNQRRWVDLEKPLKKQLDKYALEPTVYFGVVFYVPSVSQLQQEITRYQYYLQLKKDILEGSIPCTLEQA
IQLAGLAVQADFGDFDQYESQDFLQKFALFPVGWLQDEKVLEEATQKVALLHQKYRGLTAPDAEMLYMQE
VERMDGYGEESYPAKDSQGSDISIGACLEGIFVKHKNGRHPVVFRWHDIANMSHNKSFFALELANKEETI
QFQTEDMETAKYIWRLCVARHKFYRLNQCNLQTQTVTVNPIRRRSSSRMSLPKPQPYVMPPPPQLHYNGH
YTEPYASSQDNLFVPNQNGYYCHSQTSLDRAQIDLNGRIRNGSVYSAHSTNSLNNPQPYLQPSPMSSNPS
ITGSDVMRPDYLPSHRHSAVIPPSYRPTPDYETVMKQLNRGLVHAERQSHSLRNLNIGSSYAYSRPAALV
YSQPEIREHAQLPSPAAAHCPFSLSYSFHSPSPYPYPAERRPVVGAVSVPELTNAQLQAQDYPSPNIMRT
QVYRPPPPYPPPRPANSTPDLSRHLYISSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLTAARHA
QLHKRNSIEVAGLSHGLEGLRLKERTLSASAAEVAPRAVSVGSQPSVFTERTQREGPEEAEGLRYGHKKS
LSDATMLIHSSEEEEDEDFEEESGARAPPARAREPRPGLAQDPPGCPRVLLAGPLHILEPKAHVPDAEKR
MMDSSPVRTTAEAQRPWRDGLLMPSMSESDLTTSGRYRARRDSLKKRPVSDLLSGKKNIVEGLPPLGGMK
KTRVDAKKIGPLKLAALNGLSLSRVPLPDEGKEVATRATNDERCKILEQRLEQGMVFTEYERILKKRLVD
GECSTARLPENAERNRFQDVLPYDDVRVELVPTKENNTGYINASHIKVSVSGIEWDYIATQGPLQNTCQD
FWQMVWEQGIAIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKMKHLL
TGQERTVWHLQYTDWPEHGCPEDLKGFLSYLEEIQSVRRHTNSTSDPQSPNPPLLVHCSAGVGRTGVVIL
SEIMIACLEHNEVLDIPRVLDMLRQQRMMLVQTLCQYTFVYRVLIQFLKSSRLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007039
ORF Size 3522 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007039.4
RefSeq Size 4234 bp
RefSeq ORF 3525 bp
Locus ID 11099
UniProt ID Q16825
Cytogenetics 14q31.3
Domains B41, PTPc_motif, Y_phosphatase
Protein Families Druggable Genome, Phosphatase
MW 133.1 kDa
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal domain, similar to cytoskeletal- associated proteins including band 4.1, ezrin, merlin, and radixin. This PTP was shown to specially interact with BMX/ETK, a member of Tec tyrosine kinase family characterized by a multimodular structures including PH, SH3, and SH2 domains. The interaction of this PTP with BMX kinase was found to increase the activation of STAT3, but not STAT2 kinase. Studies of the similar gene in mice suggested the possible roles of this PTP in liver regeneration and spermatogenesis. provided by RefSeq, Jul 2008
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"NM_007039" in other vectors (4)
SKU Description Size Price
RC220032L3 Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21), Myc-DDK-tagged 10 ug
$1,322.00
RC220032L4 Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21), mGFP tagged 10 ug
$1,322.00
RG220032 PTPN21 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21) 10 ug
$1,222.00
SC115754 PTPN21 (untagged)-Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21) 10 ug
$1,023.00

Plasmid Map for RC220032

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220032
Circular map for RC220032

file-alt Citations

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