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LOC650293 (NM_001040071) Human Tagged ORF Clone

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SKU
RC219740
LOC650293 (Myc-DDK-tagged)-Human seven transmembrane helix receptor (LOC650293)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LOC650293
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219740 representing NM_001040071
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATCTGGTCACGGTGCTGAGGAACCTCCTCAGTATCCTGGCTGTCAGCTCTGACTCCCCCCTCCACA
CCCCCATGTACTTCTTCCTCTCCAACCTGTGCTGGGCTGACATCGGTTTCACCTCGGCCATGGTTCCCAA
GATGATTGTGGACATGCAGTCGCATAGCAGAGTCATCTCTCATGAGGGCTGCCTGACACAGATGTTTTTC
TTGGTCCTTTTTGCATGTATAGAAGGCATGATCCTGACTGTGATGGCCTATGACTGCTTTGTAGCCATCT
GTCGCCCTCTGAATTACCCAGTCATCGTGAATCCTCACCTCTGTGTCTTCTTCATTTTGATGTCCTTTTT
CCTTAGCCTGTTGGATTCCCAGCTGCACAGTTGGATTGTGTTACAATTCACAATCATCAAGAATGTGGAA
ATCTCTAATTTTGTCTGTGACCCCTCTCAACTTCTCAAACTTGCCTGTTCTGACAGCGTCATCAATAGCA
TATTCACATATTTCCATAGTACTATGTTTGCTTTTCTTCCCATTTCAGCAATCCTTTTATCTTACTATAA
AATCGTCACCTCCATTCTCAGGATTTCATCTTCAGATGGGAAGTATAAAGCCTTCTCCACCTGTGACTCT
CACCTAGCAGTTGTTTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219740 representing NM_001040071
Red=Cloning site Green=Tags(s)

MYLVTVLRNLLSILAVSSDSPLHTPMYFFLSNLCWADIGFTSAMVPKMIVDMQSHSRVISHEGCLTQMFF
LVLFACIEGMILTVMAYDCFVAICRPLNYPVIVNPHLCVFFILMSFFLSLLDSQLHSWIVLQFTIIKNVE
ISNFVCDPSQLLKLACSDSVINSIFTYFHSTMFAFLPISAILLSYYKIVTSILRISSSDGKYKAFSTCDS
HLAVVC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040071
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040071.1, NP_001035160.1
RefSeq Size 651 bp
RefSeq ORF 651 bp
Locus ID 650293
Cytogenetics 4p16.1
Protein Families Transmembrane
MW 24.2 kDa
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"NM_001040071" in other vectors (4)
SKU Description Size Price
RC219740L3 Lenti ORF clone of Human seven transmembrane helix receptor (LOC650293), Myc-DDK-tagged 10 ug
$600.00
RC219740L4 Lenti ORF clone of Human seven transmembrane helix receptor (LOC650293), mGFP tagged 10 ug
$600.00
RG219740 LOC650293 (tGFP-tagged) - Human seven transmembrane helix receptor (LOC650293) 10 ug
$500.00
SC310944 LOC650293 (untagged)-Human seven transmembrane helix receptor (LOC650293) 10 ug
$330.00

Plasmid Map for RC219740

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219740
Circular map for RC219740

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