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FBXL17 (NM_022824) Human Tagged ORF Clone

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SKU
RC219715
FBXL17 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL17
Synonyms DKFZp434C1715; F-box and leucine-rich repeat protein 17; F-box only protein 13; Fbl17; Fbx13; FBXO13; MGC161422; MGC161424
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219715 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGATAATGGCGTATGTGTTTTAGCATTTAAATGTCCTGGACTTCTTAGGTATACAGCCTACAGGT
GTAAACAGCTTTCTGACACCTCTATTATTGCGGTTGCCTCTCACTGTCCTTTACTTCAGAAAGTGCATGT
AGGCAACCAGGACAAACTCACTGATGAAGGACTCAAGCAGCTGGGCTCAAAATGCAGAGAACTCAAAGAT
ATTCATTTCGGCCAGTGTTACAAGATCTCAGATGAAGGCATGATCGTCATAGCTAAGGGCTGTCTGAAAT
TACAAAGGATATACATGCAGGAAAACAAATTAGTGACAGATCAGTCAGTGAAAGCATTTGCTGAACACTG
TCCTGAGCTTCAATATGTAGGCTTCATGGGTTGTTCAGTCACTTCTAAAGGAGTCATTCACCTAACCAAG
CTAAGAAACCTTTCCAGCTTGGACCTACGTCATATCACTGAACTGGATAATGAAACCGTGATGGAAATTG
TCAAGAGGTGCAAAAATCTTAGCTCTCTCAATCTCTGTCTGAACTGGATCATAAATGACAGGTGTGTGGA
GGTCATTGCAAAGGAAGGACAAAACCTGAAAGAGCTATATTTGGTGTCCTGTAAAATCACAGATTATGCA
CTGATAGCCATTGGGCGATACAGCATGACAATAGAGACTGTGGATGTCGGATGGTGTAAAGAAATCACAG
ACCAAGGAGCCACCCTGATTGCACAGAGCAGCAAGTCTCTGAGATATTTGGGGCTGATGAGATGTGATAA
AGTCAACGAAGTGACGGTGGAACAGCTGGTGCAGCAGTACCCCCACATCACCTTCAGCACCGTCCTGCAG
GACTGCAAGAGGACCTTGGAGAGAGCCTATCAGATGGGCTGGACCCCCAACATGTCTGCCGCCTCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219715 protein sequence
Red=Cloning site Green=Tags(s)

MSDNGVCVLAFKCPGLLRYTAYRCKQLSDTSIIAVASHCPLLQKVHVGNQDKLTDEGLKQLGSKCRELKD
IHFGQCYKISDEGMIVIAKGCLKLQRIYMQENKLVTDQSVKAFAEHCPELQYVGFMGCSVTSKGVIHLTK
LRNLSSLDLRHITELDNETVMEIVKRCKNLSSLNLCLNWIINDRCVEVIAKEGQNLKELYLVSCKITDYA
LIAIGRYSMTIETVDVGWCKEITDQGATLIAQSSKSLRYLGLMRCDKVNEVTVEQLVQQYPHITFSTVLQ
DCKRTLERAYQMGWTPNMSAASS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022824
ORF Size 909 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022824.1, NP_073735.1
RefSeq Size 3673 bp
RefSeq ORF 911 bp
Locus ID 64839
Cytogenetics 5q21.3
Protein Families Druggable Genome
MW 34.1 kDa
Summary Members of the F-box protein family, such as FBXL17, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 PubMed 15520277).supplied by OMIM, Mar 2008
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Documents
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"NM_022824" in other vectors (5)
SKU Description Size Price
RC219715L1 Lenti ORF clone of Human F-box and leucine-rich repeat protein 17 (FBXL17), Myc-DDK-tagged 10 ug
$600.00
RC219715L2 Lenti ORF clone of Human F-box and leucine-rich repeat protein 17 (FBXL17), mGFP tagged 10 ug
$600.00
RC219715L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 17 (FBXL17), Myc-DDK-tagged 10 ug
$600.00
RC219715L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 17 (FBXL17), mGFP tagged 10 ug
$600.00
RG219715 FBXL17 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 17 (FBXL17) 10 ug
$489.00 $500.00

Plasmid Map for RC219715

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219715
Circular map for RC219715

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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