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TMEM180 (MFSD13A) (NM_024789) Human Tagged ORF Clone

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SKU
RC219636
TMEM180 (Myc-DDK-tagged)-Human transmembrane protein 180 (TMEM180)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $481.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TMEM180 or NM_024789"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM180
Synonyms bA18I14.8; C10orf77; TMEM180
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219636 representing NM_024789
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTGGGTCAGCCCCAGGCCTGGTTGCTGGGTCTGCCCACAGCTGTGGTCTATGGCTCCCTGGCTC
TCTTCACCACCATCCTGCACAATGTCTTCCTGCTCTACTATGTGGACACCTTTGTCTCAGTGTACAAGAT
CAACAAAATGGCCTTCTGGGTCGGAGAGACAGTGTTTCTCCTCTGGAACAGCCTCAATGACCCTCTCTTC
GGTTGGCTCAGTGACCGGCAGTTCCTCAGCTCCCAGCCCCGGTCAGGCGCCGGGCTCTCCTCAAGGGCTG
TGGTGCTGGCCCGGGTGCAGGCCCTGGGCTGGCATGGGCCGCTGCTGGCGCTGTCGTTCCTGGCGTTCTG
GGTGCCCTGGGCCCCAGCTGGCCTGCAGTTCTTGCTGTGCCTGTGCCTCTATGATGGCTTCCTGACGCTC
GTGGACCTGCACCACCATGCCTTGCTGGCCGACCTGGCCCTCTCAGCCCACGACCGCACCCACCTCAACT
TCTACTGCTCCCTCTTCAGCGCGGCCGGCTCCCTCTCTGTCTTTGCATCCTATGCCTTTTGGAACAAGGA
GGATTTCTCCTCCTTCCGCGCTTTCTGCGTGACACTGGCTGTCAGCTCTGGGCTGGGCTTTCTGGGGGCC
ACACAGCTGCTGAGGCGGCGGGTTGAGGCGGCCCGAAAGGACCCAGGGTGCTCAGGCCTGGTTGTGGATA
GCGGCCTGTGTGGAGAGGAGCTGCTTGTGGGCAGTGAGGAGGCGGACAGCATCACCTTGGGCCGGTATCT
CCGGCAGCTGGCACGCCATCGGAACTTCCTGTGGTTCGTGAGCATGGACCTGGTGCAGGTCTTCCACTGC
CACTTCAACAGCAACTTCTTCCCTCTCTTCCTGGAGCATCTGTTGTCCGACCATATCTCCCTTTCCACGG
GCTCCATCCTGTTGGGCCTCTCCTATGTCGCTCCCCATCTCAACAACCTCTACTTCCTGTCCCTGTGCCG
GCGCTGGGGCGTCTACGCGGTGGTGCGGGGGCTCTTCCTGCTCAAGCTGGGACTTAGCCTGCTCATGTTG
TTGGCCGGCCCGGACCACCTCAGCCTGCTGTGCCTCTTCATTGCCAGCAACCGCGTCTTCACTGAGGGCA
CCTGTAAGCTGCTGACCTTGGTGGTCACTGACCTGGTAGACGAGGACCTGGTGCTGAACCACCGCAAGCA
GGCAGCCTCGGCACTCCTCTTTGGCATGGTTGCCTTGGTGACCAAGCCAGGCCAGACCTTTGCCCCGCTG
CTGGGCACCTGGCTGCTCTGTTTCTACACAGGTCATGACCTCTTCCAGCAGTCCCTCATAACCCCTGTGG
GGAGTGCCCATCCCTGGCCAGAGCCCCCAGCTCCAGCCCCTGCACAGGCCCCGACGCTCCGCCAGGGCTG
CTTCTACCTGCTGGTGCTGGTGCCCATCACCTGTGCTCTGCTGCAGCTCTTCACCTGGTCCCAGTTCACG
CTGCATGGGAGACGCCTGCACATGGTCAAGGCCCAGCGCCAGAACCTGTCACAGGCCCAAACCCTGGATG
TTAAGATGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219636 representing NM_024789
Red=Cloning site Green=Tags(s)

MGLGQPQAWLLGLPTAVVYGSLALFTTILHNVFLLYYVDTFVSVYKINKMAFWVGETVFLLWNSLNDPLF
GWLSDRQFLSSQPRSGAGLSSRAVVLARVQALGWHGPLLALSFLAFWVPWAPAGLQFLLCLCLYDGFLTL
VDLHHHALLADLALSAHDRTHLNFYCSLFSAAGSLSVFASYAFWNKEDFSSFRAFCVTLAVSSGLGFLGA
TQLLRRRVEAARKDPGCSGLVVDSGLCGEELLVGSEEADSITLGRYLRQLARHRNFLWFVSMDLVQVFHC
HFNSNFFPLFLEHLLSDHISLSTGSILLGLSYVAPHLNNLYFLSLCRRWGVYAVVRGLFLLKLGLSLLML
LAGPDHLSLLCLFIASNRVFTEGTCKLLTLVVTDLVDEDLVLNHRKQAASALLFGMVALVTKPGQTFAPL
LGTWLLCFYTGHDLFQQSLITPVGSAHPWPEPPAPAPAQAPTLRQGCFYLLVLVPITCALLQLFTWSQFT
LHGRRLHMVKAQRQNLSQAQTLDVKMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024789
ORF Size 1551 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024789.2, NP_079065.2
RefSeq Size 2846 bp
RefSeq ORF 1554 bp
Locus ID 79847
UniProt ID Q14CX5
Cytogenetics 10q24.32
Protein Families Transmembrane
MW 57.2 kDa
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Documents
Resources
"NM_024789" in other vectors (6)
SKU Description Size Price
RC219636L1 Lenti ORF clone of Human transmembrane protein 180 (TMEM180), Myc-DDK-tagged 10 ug
$781.00
RC219636L2 Lenti ORF clone of Human transmembrane protein 180 (TMEM180), mGFP tagged 10 ug
$781.00
RC219636L3 Lenti ORF clone of Human transmembrane protein 180 (TMEM180), Myc-DDK-tagged 10 ug
$781.00
RC219636L4 Lenti ORF clone of Human transmembrane protein 180 (TMEM180), mGFP tagged 10 ug
$781.00
RG219636 TMEM180 (tGFP-tagged) - Human transmembrane protein 180 (TMEM180) 10 ug
$681.00
SC313814 TMEM180 (untagged)-Human transmembrane protein 180 (TMEM180) 10 ug
$530.00

Plasmid Map for RC219636

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219636
Circular map for RC219636

file-alt Citations

Topological analysis of TMEM180, a newly identified membrane protein that is highly expressed in colorectal cancer cells
Anzai, Matsumura
Biochem. Biophys. Res. Commun. (2019) 520 (3), 566-572 DOI: 10.1016/j.bbrc.2019.10.070
AI Summary: OriGene’s pCMV-TMEM180 plasmid was used as the template to PCR amplify the human TMEM180 gene for subsequent plasmid construction and topological analysis in this study.
Snippet: atmosphere. 2.2. Plasmid construction. The human TMEM180 gene was PCR amplified from plasmid pCMV-TMEM180 (OriGene) and fused with an EcoR1 restriction site in the 5′ region and a BamH1 restriction site in the 3' region
Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule
Yasunaga, Saijou, Hanaoka et al
Cancer Sci. (2019) 110 (2), 761-770 DOI: 10.1111/cas.13907
AI Summary: OriGene’s pCMV-TMEM180 plasmid was used to overexpress the TMEM180 gene in DLD-1 cells, facilitating functional studies of this novel colorectal cancer marker in the paper.
Snippet: For overexpression of the TMEM180 gene, plasmid pCMV‐TMEM180 (OriGene, Rockville, MD, USA) was transfected into DLD‐1 cells (DLD‐1‐OE) using Lipofectamine LTX (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's protocol
FigureFigureFigure
Showing 1-2 of 2 papers.
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