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NAALAD2 (NM_005467) Human Tagged ORF Clone

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SKU
RC219582
NAALAD2 (Myc-DDK-tagged)-Human N-acetylated alpha-linked acidic dipeptidase 2 (NAALAD2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $678.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NAALAD2
Synonyms GCPIII; GPCIII; NAADALASE2; NAALADASE2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219582 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAATCCAGGGGCCGTCTGTACCTTTGGATGTGCTTGGCTGCTGCGCTGGCATCTTTCCTGATGG
GATTTATGGTGGGCTGGTTTATTAAGCCTCTCAAAGAAACAACCACTTCTGTGCGCTATCATCAAAGTAT
ACGGTGGAAACTGGTATCCGAAATGAAAGCTGAAAACATCAAATCATTTCTTCGTTCTTTTACAAAGCTT
CCTCATCTGGCAGGAACAGAACAAAATTTCTTGCTTGCCAAGAAAATCCAAACCCAGTGGAAGAAATTTG
GACTAGATTCAGCCAAGTTGATTCATTATGATGTCCTCTTATCTTACCCCAATGAGACAAATGCCAACTA
TATATCGATTGTGGATGAACATGAAACTGAGATTTTCAAAACATCATACCTTGAACCACCACCAGATGGC
TATGAGAATGTTACAAATATTGTGCCACCATATAATGCTTTCTCAGCCCAAGGCATGCCAGAGGGAGATC
TTGTATATGTGAACTATGCTCGCACTGAAGACTTTTTCAAACTAGAAAGAGAGATGGGCATCAACTGTAC
TGGGAAGATTGTTATTGCAAGATATGGAAAAATCTTCAGAGGAAATAAAGTTAAAAATGCCATGTTAGCA
GGAGCCATAGGAATCATCTTGTACTCAGATCCAGCTGACTACTTTGCTCCTGAGGTACAGCCATATCCCA
AAGGATGGAATCTTCCTGGAACTGCAGCCCAGAGAGGAAATGTGTTAAATTTGAATGGTGCTGGTGACCC
ACTCACTCCAGGCTATCCAGCAAAAGAATACACTTTCAGACTTGATGTTGAAGAAGGAGTGGGAATCCCC
CGAATACCTGTACATCCCATTGGATATAATGATGCAGAAATATTATTACGCTACTTGGGAGGAATTGCTC
CACCAGATAAGAGTTGGAAGGGAGCCCTTAATGTGAGTTATAGTATCGGACCTGGCTTTACAGGGAGTGA
TTCTTTCAGGAAGGTTAGAATGCATGTTTATAACATCAATAAAATTACAAGGATTTACAATGTAGTTGGA
ACTATCAGAGGATCTGTGGAACCTGACAGGTATGTTATTCTGGGAGGTCACCGGGACTCCTGGGTATTTG
GAGCTATTGACCCAACCAGTGGGGTTGCTGTTTTGCAAGAAATTGCCCGGAGTTTTGGAAAACTGATGAG
TAAAGGCTGGAGACCTAGAAGAACTATCATTTTTGCCAGCTGGGATGCAGAAGAATTTGGACTTCTGGGT
TCCACAGAATGGGCTGAGGAGAATGTCAAAATACTCCAGGAGAGAAGCATTGCTTATATCAACTCGGATT
CATCTATAGAAGGCAATTATACTCTCAGAGTTGACTGTACTCCCCTTCTTTACCAATTAGTGTATAAACT
GACAAAAGAGATCCCCAGCCCTGATGATGGGTTTGAGAGTAAATCACTGTATGAAAGCTGGTTGGAAAAA
GACCCTTCACCTGAAAATAAAAATTTGCCTAGAATCAATAAGCTGGGATCTGGAAGTGACTTTGAAGCTT
ATTTTCAGAGACTTGGAATTGCTTCAGGCAGAGCCCGTTACACTAAGAATAAGAAAACAGATAAGTACAG
CAGCTACCCAGTGTACCACACAATTTATGAGACATTTGAATTGGTAGAGAAATTTTATGACCCCACATTT
AAAAAACAACTTTCTGTGGCTCAATTACGAGGAGCACTGGTATATGAGCTTGTGGATTCTAAAATCATTC
CTTTTAATATTCAAGACTATGCAGAAGCTTTGAAAAACTATGCAGCAAGTATCTATAATCTATCTAAGAA
ACATGATCAACAATTGACAGACCATGGAGTATCATTTGACTCCTTATTTTCTGCTGTGAAAAACTTCTCA
GAGGCTGCTTCAGATTTTCATAAACGACTTATACAAGTTGATCTTAACAATCCCATTGCAGTGAGAATGA
TGAATGACCAACTGATGCTCCTGGAAAGAGCATTCATCGATCCTCTTGGTTTACCAGGAAAGCTGTTCTA
TAGGCACATCATATTTGCTCCAAGTAGCCACAACAAATATGCTGGAGAATCATTTCCTGGAATCTATGAT
GCTATCTTTGATATTGAAAATAAAGCCAACTCTCGTTTGGCCTGGAAAGAAGTAAAGAAACATATTTCTA
TTGCAGCTTTTACAATTCAAGCAGCAGCAGGAACTCTGAAAGAAGTATTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219582 protein sequence
Red=Cloning site Green=Tags(s)

MAESRGRLYLWMCLAAALASFLMGFMVGWFIKPLKETTTSVRYHQSIRWKLVSEMKAENIKSFLRSFTKL
PHLAGTEQNFLLAKKIQTQWKKFGLDSAKLIHYDVLLSYPNETNANYISIVDEHETEIFKTSYLEPPPDG
YENVTNIVPPYNAFSAQGMPEGDLVYVNYARTEDFFKLEREMGINCTGKIVIARYGKIFRGNKVKNAMLA
GAIGIILYSDPADYFAPEVQPYPKGWNLPGTAAQRGNVLNLNGAGDPLTPGYPAKEYTFRLDVEEGVGIP
RIPVHPIGYNDAEILLRYLGGIAPPDKSWKGALNVSYSIGPGFTGSDSFRKVRMHVYNINKITRIYNVVG
TIRGSVEPDRYVILGGHRDSWVFGAIDPTSGVAVLQEIARSFGKLMSKGWRPRRTIIFASWDAEEFGLLG
STEWAEENVKILQERSIAYINSDSSIEGNYTLRVDCTPLLYQLVYKLTKEIPSPDDGFESKSLYESWLEK
DPSPENKNLPRINKLGSGSDFEAYFQRLGIASGRARYTKNKKTDKYSSYPVYHTIYETFELVEKFYDPTF
KKQLSVAQLRGALVYELVDSKIIPFNIQDYAEALKNYAASIYNLSKKHDQQLTDHGVSFDSLFSAVKNFS
EAASDFHKRLIQVDLNNPIAVRMMNDQLMLLERAFIDPLGLPGKLFYRHIIFAPSSHNKYAGESFPGIYD
AIFDIENKANSRLAWKEVKKHISIAAFTIQAAAGTLKEVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005467
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005467.4
RefSeq Size 3196 bp
RefSeq ORF 2223 bp
Locus ID 10003
UniProt ID Q9Y3Q0
Cytogenetics 11q14.3
Domains PA, Peptidase_M28, TFR_dimer
Protein Families Druggable Genome, Transmembrane
MW 83.6 kDa
Summary This gene is a member of the N-acetylated alpha-linked acidic dipeptidase (NAALADase) gene family. The representative member of this family is the gene encoding human prostate-specific membrane antigen (PSM), which is a marker of prostatic carcinomas and is the first to be shown to possess NAALADase activity. NAALADase cleaves N-acetyl-L-aspartate-L-glutamate (NAAG), which is a neuropeptide expressed both in the central nervous systems and in the periphery and is thought to function as a neurotransmitter. The product of this gene is a type II integral membrane protein. Transient transfection of this gene confers both NAALADase and dipetidyl peptidase IV activities to mammalian cells. This gene is highly expressed in ovary and testis as well as within discrete brain areas. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2014
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"NM_005467" in other vectors (4)
SKU Description Size Price
RC219582L3 Lenti ORF clone of Human N-acetylated alpha-linked acidic dipeptidase 2 (NAALAD2), Myc-DDK-tagged 10 ug
$978.00
RC219582L4 Lenti ORF clone of Human N-acetylated alpha-linked acidic dipeptidase 2 (NAALAD2), mGFP tagged 10 ug
$978.00
RG219582 NAALAD2 (tGFP-tagged) - Human N-acetylated alpha-linked acidic dipeptidase 2 (NAALAD2) 10 ug
$789.00 $878.00
SC310136 NAALAD2 (untagged)-Human N-acetylated alpha-linked acidic dipeptidase 2 (NAALAD2) 10 ug
$746.00

Plasmid Map for RC219582

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219582
Circular map for RC219582

file-alt Citations

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