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ZBTB1 (NM_014950) Human Tagged ORF Clone

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SKU
RC219510
ZBTB1 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $599.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZBTB1
Synonyms ZNF909
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219510 representing NM_014950
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAGCCCAGCCACAGCAGCTATGTCCTTCAGCAGCTAAACAACCAAAGAGAATGGGGTTTTCTCT
GTGACTGCTGTATTGCAATTGATGACATTTACTTTCAAGCACACAAGGCAGTTCTAGCTGCCTGTAGCTC
CTATTTTAGAATGTTTTTCATGAACCATCAGCATAGTACTGCACAACTGAATCTCAGCAACATGAAAATT
AGTGCAGAATGTTTTGATCTCATTTTGCAGTTTATGTATTTAGGAAAAATTATGACAGCTCCCTCCAGTT
TTGAGCAGTTTAAAGTGGCAATGAACTACCTACAGCTATACAATGTTCCTGACTGTTTAGAAGACATCCA
GGATGCAGATTGTTCTAGTTCAAAATGTTCCTCTTCTGCTTCCAGCAAACAGAACAGCAAAATGATATTT
GGGGTAAGAATGTATGAAGATACTGTGGCTCGAAATGGCAATGAAGCCAACAGGTGGTGTGCAGAGCCAA
GTTCAACGGTAAATACACCACATAATAGAGAGGCTGATGAAGAGTCTTTACAATTAGGTAATTTTCCTGA
GCCACTATTTGATGTATGTAAAAAAAGTTCCGTGTCCAAATTATCTACTCCAAAAGAACGTGTGTCAAGA
CGCTTTGGGCGGAGTTTTACCTGTGATAGCTGTGGATTTGGCTTTAGCTGTGAAAAATTATTAGATGAGC
ATGTGCTAACCTGTACTAACAGACATTTATACCAAAACACAAGATCTTACCATAGAATAGTAGATATTAG
AGATGGAAAAGACAGTAACATCAAAGCTGAATTTGGTGAAAAAGATTCTTCCAAAACATTTTCTGCACAG
ACGGACAAATACAGAGGAGACACAAGCCAGGCTGCTGATGATTCAGCTTCAACCACTGGAAGCAGAAAAA
GTAGCACAGTGGAGTCTGAAATAGCAAGCGAAGAGAAAAGCAGAGCTGCTGAGAGGAAAAGGATTATTAT
TAAGATGGAGCCAGAAGATATTCCTACAGATGAACTGAAAGACTTTAACATTATTAAAGTTACTGATAAA
GACTGTAATGAATCCACTGACAATGATGAATTAGAAGATGAACCTGAAGAGCCATTTTATAGATACTATG
TTGAAGAAGATGTCAGCATAAAAAAAAGTGGTAGGAAAACTCTAAAACCTCGAATGTCAGTAAGTGCTGA
TGAAAGAGGTGGTTTAGAGAATATGAGGCCCCCTAACAACAGCAGTCCAGTACAAGAGGATGCTGAAAAT
GCATCTTGTGAGCTGTGTGGACTTACAATAACCGAGGAGGACCTGTCATCTCATTACTTAGCCAAACACA
TTGAAAATATCTGTGCATGTGGTAAATGTGGACAAATACTTGTAAAGGGTAGGCAGCTTCAGGAACATGC
TCAACGATGTGGCGAGCCCCAAGATCTGACCATGAATGGGTTAGGAAATACTGAGGAGAAAATGGACTTG
GAAGAGAATCCTGATGAGCAGTCCGAAATAAGAGATATGTTTGTTGAAATGCTGGATGATTTTAGGGACA
ATCATTACCAGATAAACAGTATCCAAAAAAAGCAGTTATTTAAACATTCTGCCTGCCCTTTTCGATGTCC
TAATTGTGGCCAGCGTTTTGAAACTGAAAATCTAGTGGTTGAACATATGTCTAGCTGCTTAGATCAAGAT
ATGTTTAAGAGTGCCATCATGGAAGAAAATGAAAGAGATCACAGACGAAAGCATTTTTGTAATCTGTGTG
GAAAAGGATTTTATCAGCGGTGTCACTTAAGAGAACACTATACTGTTCATACTAAGGAAAAACAGTTTGT
TTGTCAAACATGTGGAAAGCAGTTTTTAAGAGAGCGTCAGTTGCGACTGCACAATGATATGCACAAAGGC
ATGGCCAGTGGTGAAATAGGGCCTTCTAAACCTGTGGAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219510 representing NM_014950
Red=Cloning site Green=Tags(s)

MAKPSHSSYVLQQLNNQREWGFLCDCCIAIDDIYFQAHKAVLAACSSYFRMFFMNHQHSTAQLNLSNMKI
SAECFDLILQFMYLGKIMTAPSSFEQFKVAMNYLQLYNVPDCLEDIQDADCSSSKCSSSASSKQNSKMIF
GVRMYEDTVARNGNEANRWCAEPSSTVNTPHNREADEESLQLGNFPEPLFDVCKKSSVSKLSTPKERVSR
RFGRSFTCDSCGFGFSCEKLLDEHVLTCTNRHLYQNTRSYHRIVDIRDGKDSNIKAEFGEKDSSKTFSAQ
TDKYRGDTSQAADDSASTTGSRKSSTVESEIASEEKSRAAERKRIIIKMEPEDIPTDELKDFNIIKVTDK
DCNESTDNDELEDEPEEPFYRYYVEEDVSIKKSGRKTLKPRMSVSADERGGLENMRPPNNSSPVQEDAEN
ASCELCGLTITEEDLSSHYLAKHIENICACGKCGQILVKGRQLQEHAQRCGEPQDLTMNGLGNTEEKMDL
EENPDEQSEIRDMFVEMLDDFRDNHYQINSIQKKQLFKHSACPFRCPNCGQRFETENLVVEHMSSCLDQD
MFKSAIMEENERDHRRKHFCNLCGKGFYQRCHLREHYTVHTKEKQFVCQTCGKQFLRERQLRLHNDMHKG
MASGEIGPSKPVEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014950
ORF Size 1932 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014950.3
RefSeq Size 3990 bp
RefSeq ORF 1935 bp
Locus ID 22890
UniProt ID Q9Y2K1
Cytogenetics 14q23.3
Domains BTB, zf-C2H2
Protein Families Transcription Factors
MW 73.5 kDa
Summary Acts as a transcriptional repressor (PubMed:20797634). Represses cAMP-responsive element (CRE)-mediated transcriptional activation (PubMed:21706167). In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis (PubMed:24657165). Plays a key role in the transcriptional regulation of T lymphocyte development (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_014950" in other vectors (4)
SKU Description Size Price
RC219510L3 Lenti ORF clone of Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2, Myc-DDK-tagged 10 ug
$899.00
RC219510L4 Lenti ORF clone of Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2, mGFP tagged 10 ug
$899.00
RG219510 ZBTB1 (tGFP-tagged) - Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2 10 ug
$799.00
SC314314 ZBTB1 (untagged)-Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2 10 ug
$660.00

Plasmid Map for RC219510

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219510
Circular map for RC219510

file-alt Citations

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