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TSGA10 (NM_182911) Human Tagged ORF Clone

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SKU
RC219495
TSGA10 (Myc-DDK-tagged)-Human testis specific, 10 (TSGA10), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $639.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TSGA10
Synonyms CEP4L; CT79; SPGF26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219495 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCGAAGTAGGTCTAAAAGTCCAAGACGCCCATCACCAACTGCCCGGGGTGCAAACTGTGATGTAG
AACTTTTGAAGACAACAACAAGAGATCGTGAAGAACTTAAATGCATGCTGGAAAAATATGAGCGCCATTT
GGCAGAAATTCAGGGTAATGTCAAGGTTCTTAAATCTGAGAGAGACAAGATCTTCCTTCTTTATGAACAG
GCACAGGAAGAAATTACCCGACTTCGACGAGAAATGATGAAAAGCTGTAAGAGTCCTAAATCAACAACGG
CACATGCTATTCTCCGGCGAGTGGAGACTGAAAGAGATGTAGCCTTTACTGATTTACGAAGAATGACCAC
AGAACGAGATAGTCTAAGGGAGAGGCTAAAGATTGCTCAAGAGACAGCATTTAATGAGAAGGCTCACCTG
GAACAAAGGATAGAGGAGCTGGAGTGTACAGTTCATAATCTTGATGATGAACGTATGGAGCAAATGTCAA
ATATGACTTTGATGAAGGAAACCATAAGCACTGTGGAAAAAGAAATGAAATCACTAGCAAGAAAGGCAAT
GGATACCGAAAGTGAACTTGGCAGACAAAAAGCAGAGAATAATTCTTTGAGACTTTTGTATGAAAACACA
GAAAAAGATCTTTCTGATACTCAGCGACACCTTGCTAAGAAAAAATATGAGCTACAGCTTACTCAGGAGA
AAATTATGTGCTTGGATGAAAAAATTGATAACTTTACAAGGCAAAATATTGCACAGCGAGAAGAAATCAG
CATTCTTGGTGGAACCCTCAATGATCTGGCTAAAGAAAAGGAATGCATGCAAGCATGTTTGGATAAAAAA
TCTGAGAATATTGCATCCCTTGGAGAGAGTTTGGCAATGAAAGAAAAGACCATTTCAGGCATGAAGAATA
TCATTGCTGAGATGGAACAGGCATCAAGACAGTGTACTGAGGCCCTAATTGTGTGTGAACAAGACGTTTC
CAGAATGCGTCGGCAATTGGATGAGACAAATGATGAGCTGGCCCAGATCGCCAGGGAAAGAGATATCTTG
GCTCATGACAATGACAATCTCCAGGAACAGTTTGCTAAAGCTAAACAAGAAAACCAGGCACTGTCCAAAA
AATTGAATGACACTCATAATGAACTTAATGACATAAAACAGAAGGTTCAAGATACTAATTTGGAGGTTAA
CAAGCTGAAGAATATATTAAAGTCTGAAGAATCTGAGAACCGGCAAATGATGGAACAACTTCGAAAAGCC
AATGAAGATGCTGAAAACTGGGAAAATAAAGCCCGTCAATCAGAGGCAGATAACAATACCCTCAAACTGG
AACTTATCACTGCTGAGGCAGAGGGTAACAGATTAAAAGAAAAAGTAGATTCCCTCAACAGAGAGGTTGA
GCAACACTTAAATGCAGAAAGGTCTTACAAGTCCCAGATTTCTACCTTACATAAATCTGTTGTAAAAATG
GAAGAGGAGCTTCAGAAGGTTCAGTTTGAAAAAGTGTCCGCTCTTGCAGATTTGTCTTCTACTAGGGAAC
TCTGTATTAAACTTGACTCAAGCAAAGAACTTCTTAATCGACAGCTGGTTGCTAAAGATCAAGAAATAGA
AATGAGGGAGAATGAGTTAGATTCTGCTCATTCTGAAATTGAACTCCTGAGGAGTCAGATGGCAAATGAG
AGAATCTCCATGCAGAATCTAGAAGCTTTGCTGGTGGCCAATCGAGACAAAGAATATCAGTCTCAGATAG
CACTTCAAGAAAAAGAATCTGAAATTCAGCTTCTTAAAGAACACCTTTGTTTGGCAGAAAATAAAATGGC
CATCCAGAGTAGAGATGTGGCCCAGTTCAGAAATGTTGTCACACAATTGGAAGCTGATTTAGACATTACC
AAAAGACAACTAGGAACAGAGCGCTTTGAAAGGGAGAGGGCCGTACAAGAACTTCGCCGCCAAAATTATT
CAAGTAATGCTTATCATATGAGTTCTACAATGAAGCCAAATACAAAATGTCATTCACCAGAACGTGCTCA
CCATCGATCTCCTGACCGAGGCCTAGATCGATCATTAGAAGAGAATCTTTGCTACAGAGATTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219495 protein sequence
Red=Cloning site Green=Tags(s)

MMRSRSKSPRRPSPTARGANCDVELLKTTTRDREELKCMLEKYERHLAEIQGNVKVLKSERDKIFLLYEQ
AQEEITRLRREMMKSCKSPKSTTAHAILRRVETERDVAFTDLRRMTTERDSLRERLKIAQETAFNEKAHL
EQRIEELECTVHNLDDERMEQMSNMTLMKETISTVEKEMKSLARKAMDTESELGRQKAENNSLRLLYENT
EKDLSDTQRHLAKKKYELQLTQEKIMCLDEKIDNFTRQNIAQREEISILGGTLNDLAKEKECMQACLDKK
SENIASLGESLAMKEKTISGMKNIIAEMEQASRQCTEALIVCEQDVSRMRRQLDETNDELAQIARERDIL
AHDNDNLQEQFAKAKQENQALSKKLNDTHNELNDIKQKVQDTNLEVNKLKNILKSEESENRQMMEQLRKA
NEDAENWENKARQSEADNNTLKLELITAEAEGNRLKEKVDSLNREVEQHLNAERSYKSQISTLHKSVVKM
EEELQKVQFEKVSALADLSSTRELCIKLDSSKELLNRQLVAKDQEIEMRENELDSAHSEIELLRSQMANE
RISMQNLEALLVANRDKEYQSQIALQEKESEIQLLKEHLCLAENKMAIQSRDVAQFRNVVTQLEADLDIT
KRQLGTERFERERAVQELRRQNYSSNAYHMSSTMKPNTKCHSPERAHHRSPDRGLDRSLEENLCYRDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182911
ORF Size 2094 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182911.2
RefSeq Size 3664 bp
RefSeq ORF 2097 bp
Locus ID 80705
UniProt ID Q9BZW7, A0A218MIY9
Cytogenetics 2q11.2
MW 81.4 kDa
Summary Plays a role in spermatogenesis (PubMed:28905369). When overexpressed, prevents nuclear localization of HIF1A (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_182911" in other vectors (4)
SKU Description Size Price
RC219495L3 Lenti ORF clone of Human testis specific, 10 (TSGA10), transcript variant 2, Myc-DDK-tagged 10 ug
$939.00
RC219495L4 Lenti ORF clone of Human testis specific, 10 (TSGA10), transcript variant 2, mGFP tagged 10 ug
$939.00
RG219495 TSGA10 (tGFP-tagged) - Human testis specific, 10 (TSGA10), transcript variant 2 10 ug
$839.00
SC307535 TSGA10 (untagged)-Human testis specific, 10 (TSGA10), transcript variant 2 10 ug
$704.00

Plasmid Map for RC219495

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219495
Circular map for RC219495

file-alt Citations

Tsga10 is essential for arrangement of mitochondrial sheath and male fertility in mice
Luo, Hou, Wang et al
Andrology (2020)
Cited by 43
AI Summary: OriGene contributed to this study by providing a plasmid encoding TSGA10, which was used to analyze the protein's function in spermatogenesis and male fertility in mice.
Snippet: Germline transmission was confirmed by subsequent PCR genotyping. Plasmids construction A plasmid encoding TSGA10 was purchased from OriGene. Full-length cDNAs encoding GRP78 and NSUN2 were Accepted Article Page 4. This article is protected by copyright
Showing 1-1 of 1 papers.
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