LGI4 (NM_139284) Human Tagged ORF Clone
$500.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LGI4 |
| Synonyms | AMC1; AMCNMY; LGIL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_139284 |
| ORF Size | 1611 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_139284.3 |
| RefSeq Size | 2814 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 163175 |
| UniProt ID | Q8N135 |
| Cytogenetics | 19q13.11 |
| Domains | EPTP, LRR, LRRCT, LRR_TYP |
| MW | 59 kDa |
| Summary | Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_139284" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219293L3 | Lenti ORF clone of Human leucine-rich repeat LGI family, member 4 (LGI4), Myc-DDK-tagged | 10 ug |
$800.00
|
|
| RC219293L4 | Lenti ORF clone of Human leucine-rich repeat LGI family, member 4 (LGI4), mGFP tagged | 10 ug |
$800.00
|
|
| RG219293 | LGI4 (tGFP-tagged) - Human leucine-rich repeat LGI family, member 4 (LGI4) | 10 ug |
$700.00
|
|
| SC313909 | LGI4 (untagged)-Human leucine-rich repeat LGI family, member 4 (LGI4) | 10 ug |
$551.00
|
|
Citations
Clinical Spectrum, Pathology, and Mechanisms of Anti-LGI4 Antibody-Positive Autoimmune Nodopathy
Zhang, Kira, Yokote et al
Neurol Neuroimmunol Neuroinflamm (2025) 12 (6), e200504 DOI: 10.1212/NXI.0000000000200504
Zhang, Kira, Yokote et al
Neurol Neuroimmunol Neuroinflamm (2025) 12 (6), e200504 DOI: 10.1212/NXI.0000000000200504
AI Summary: OriGene’s LGI4-FLAG plasmid (ID RC219293) was used to transfect HEK293T cells in the development of a live cell-based assay for detecting anti-LGI4 antibodies, enabling the identification and characterization of autoimmune nodopathy patients in this study.
Snippet: escence Assay for Anti-LGI4 Antibodies Human embryonic kidney 293T cells, maintained in a medium containing 10% fetal bovine serum (FBS), were transfected with both an LGI4-FLAG plasmid (ID RC219293, OriGene Technologies, Inc.) and a disintegrin and metalloprotease domain–containing protein 22 (ADAM22)-HA plasmid (constructed by MF and YF), or with the ADAM22-HA plasmid only, using the Lipofecta
Anti-LGI4 Antibody Is a Novel Juxtaparanodal Autoantibody for Chronic Inflammatory Demyelinating Polyneuropathy
Zhang, Kira, Ogata et al
Neurol Neuroimmunol Neuroinflamm (2023) 10 (2) DOI: 10.1212/NXI.0000000000200081
Zhang, Kira, Ogata et al
Neurol Neuroimmunol Neuroinflamm (2023) 10 (2) DOI: 10.1212/NXI.0000000000200081
AI Summary: OriGene’s LGI4-Flag plasmid (ID RC219293) was used to transfect HEK293T cells, enabling the identification and characterization of anti-LGI4 autoantibodies in patients with chronic inflammatory demyelinating polyneuropathy.
Snippet: … The cells were transfected with both an LGI4-Flag plasmid (ID RC219293, OriGene Technologies, Inc., Rockville, MD) and a disintegrin and metalloprotease domain–containing protein 22 (ADAM22)–HA plasmid (constructed by M.F. … The cells were transfected with both an LGI4-Flag plasmid (ID RC219293, OriGene Technologies, Inc., Rockville, MD) and a disintegrin and metalloprotease domain–containing prote
Showing 1-2 of 2 papers.
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