IGFL3 (NM_207393) Human Tagged ORF Clone

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SKU
RC219195
IGFL3 (Myc-DDK-tagged)-Human IGF-like family member 3 (IGFL3)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IGFL3
Synonyms UNQ483
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219195 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCCACGATGCTGCATCTTGGCTCTTGTCTGCTGGATAACAGTCTTCCTCCTCCAGTGTTCAAAAG
GAACTACAGACGCTCCTGTTGGCTCAGGACTGTGGCTGTGCCAGCCGACACCCAGGTGTGGGAACAAGAT
CTACAACCCTTCAGAGCAGTGCTGTTATGATGATGCCATCTTATCCTTAAAGGAGACCCGCCGCTGTGGC
TCCACCTGCACCTTCTGGCCCTGCTTTGAGCTCTGCTGTCCCGAGTCTTTTGGCCCCCAGCAGAAGTTTC
TTGTGAAGTTGAGGGTTCTGGGTATGAAGTCTCAGTGTCACTTATCTCCCATCTCCCGGAGCTGTACCAG
GAACAGGAGGCACGTCCTGTACCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219195 protein sequence
Red=Cloning site Green=Tags(s)

MRPRCCILALVCWITVFLLQCSKGTTDAPVGSGLWLCQPTPRCGNKIYNPSEQCCYDDAILSLKETRRCG
STCTFWPCFELCCPESFGPQQKFLVKLRVLGMKSQCHLSPISRSCTRNRRHVLYP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207393
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207393.2
RefSeq Size 662 bp
RefSeq ORF 378 bp
Locus ID 388555
UniProt ID Q6UXB1
Cytogenetics 19q13.32
Protein Families Secreted Protein
MW 14.2 kDa
Summary IGFL3 belongs to the insulin-like growth factor (IGF; see MIM 147440) family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth (Emtage et al., 2006 PubMed 16890402).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_207393" in other vectors (4)
SKU Description Size Price
RC219195L3 Lenti ORF clone of Human IGF-like family member 3 (IGFL3), Myc-DDK-tagged 10 ug
$450.00
RC219195L4 Lenti ORF clone of Human IGF-like family member 3 (IGFL3), mGFP tagged 10 ug
$450.00
RG219195 IGFL3 (tGFP-tagged) - Human IGF-like family member 3 (IGFL3) 10 ug
$489.00
SC308508 IGFL3 (untagged)-Human IGF-like family member 3 (IGFL3) 10 ug
$165.00

Plasmid Map for RC219195

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Cloning scheme for RC219195

Circular map for RC219195

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