ELP4 (NM_019040) Human Tagged ORF Clone

  • TrueORF®
SKU
RC219128
ELP4 (Myc-DDK-tagged)-Human elongation protein 4 homolog (S. cerevisiae) (ELP4)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELP4
Synonyms AN; AN2; C11orf19; dJ68P15A.1; hELP4; PAX6NEB; PAXNEB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219128 representing NM_019040
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGTGGCAACCTGCGGTAGTGTTGCCGCGAGTACTGGGTCTGCAGTGGCGACAGCCAGCAAGA
GCAACGTCACCAGTTTCCAGAGGAGGGGTCCTAGAGCCAGCGTGACCAACGACAGCGGCCCTCGACTGGT
GTCCATTGCGGGCACGCGACCGTCGGTGCGGAATGGACAGCTGCTGGTATCAACCGGGCTCCCAGCCCTA
GACCAGCTCTTAGGTGGAGGTTTAGCCGTTGGAACAGTTCTTCTAATTGAGGAGGATAAATATAATATTT
ACTCACCTTTGCTCTTCAAGTATTTCCTGGCAGAAGGAATTGTCAATGGGCATACTTTGTTGGTTGCATC
TGCTAAAGAGGATCCTGCCAACATTTTACAGGAACTTCCAGCACCATTACTTGATGATAAATGTAAAAAG
GAATTTGATGAAGATGTATACAATCATAAAACACCAGAATCTAATATTAAGATGAAAATAGCTTGGCGTT
ACCAGTTATTACCCAAGATGGAGATTGGACCAGTATCATCTTCAAGATTTGGTCACTATTATGATGCATC
AAAAAGAATGCCACAAGAACTAATTGAGGCTTCAAATTGGCATGGATTTTTTCTTCCAGAGAAAATATCT
TCAACTCTCAAAGTAGAACCCTGTTCTTTGACCCCTGGCTACACAAAGCTGCTTCAGTTTATCCAGAACA
TCATTTATGAGGAAGGATTTGATGGATCCAATCCTCAGAAAAAACAGAGAAACATTTTAAGAATAGGAAT
TCAGAATCTTGGCTCACCTTTATGGGGAGACGATATTTGCTGTGCAGAAAATGGTGGCAACAGTCACAGC
CTTACCAAGTTCCTCTATGTTCTCCGTGGTCTTCTGAGAACCTCTCTTTCAGCCTGCATCATCACAATGC
CAACACATCTGATCCAGAATAAAGCCATTATTGCCCGTGTCACAACCTTGTCAGATGTAGTAGTTGGTCT
GGAATCATTTATTGGTTCTGAGAGAGAAACTAACCCATTGTATAAGGATTATCATGGATTGATTCATATA
CGGCAGATTCCTCGGCTTAATAACTTGATCTGTGATGAATCAGATGTCAAAGACTTAGCTTTTAAATTAA
AAAGGAAGCTATTCACCATTGAGCGACTGCATTTGCCTCCAGACTTGTCAGACACAGTGAGCCGCTCAAG
CAAAATGGATCTGGCAGAATCCGCCAAGCGGCTGGGCCCAGGCTGTGGCATGATGGCCGGAGGCAAGAAG
CACCTGGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219128 representing NM_019040
Red=Cloning site Green=Tags(s)

MAAVATCGSVAASTGSAVATASKSNVTSFQRRGPRASVTNDSGPRLVSIAGTRPSVRNGQLLVSTGLPAL
DQLLGGGLAVGTVLLIEEDKYNIYSPLLFKYFLAEGIVNGHTLLVASAKEDPANILQELPAPLLDDKCKK
EFDEDVYNHKTPESNIKMKIAWRYQLLPKMEIGPVSSSRFGHYYDASKRMPQELIEASNWHGFFLPEKIS
STLKVEPCSLTPGYTKLLQFIQNIIYEEGFDGSNPQKKQRNILRIGIQNLGSPLWGDDICCAENGGNSHS
LTKFLYVLRGLLRTSLSACIITMPTHLIQNKAIIARVTTLSDVVVGLESFIGSERETNPLYKDYHGLIHI
RQIPRLNNLICDESDVKDLAFKLKRKLFTIERLHLPPDLSDTVSRSSKMDLAESAKRLGPGCGMMAGGKK
HLDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019040
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019040.5
RefSeq Size 2341 bp
RefSeq ORF 1275 bp
Locus ID 26610
UniProt ID Q96EB1
Cytogenetics 11p13
MW 47 kDa
Summary This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. This gene has also been associated with Rolandic epilepsy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2013
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Documents
Resources
"NM_019040" in other vectors (4)
SKU Description Size Price
RC219128L3 Lenti-ORF clone of ELP4 (Myc-DDK-tagged)-Human elongation protein 4 homolog (S. cerevisiae) (ELP4) 10 ug
$757.00
RC219128L4 Lenti-ORF clone of ELP4 (mGFP-tagged)-Human elongation protein 4 homolog (S. cerevisiae) (ELP4) 10 ug
$757.00
RG219128 ELP4 (tGFP-tagged) - Human elongation protein 4 homolog (S. cerevisiae) (ELP4) 10 ug
$489.00 $657.00
SC113303 ELP4 (untagged)-Human elongation protein 4 homolog (S. cerevisiae) (ELP4) 10 ug
$499.00

Plasmid Map for RC219128

Download sequence for

Cloning scheme for RC219128

Circular map for RC219128

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