ZP2 (NM_003460) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC219115L3
Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), Myc-DDK-tagged
  $982.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZP2
Synonyms OOMD6; Zp-2; ZPA
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC219115).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_003460
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003460.1
RefSeq Size 2266 bp
RefSeq ORF 2238 bp
Locus ID 7783
UniProt ID Q05996
Cytogenetics 16p12.3-p12.2
Domains zona_pellucida
Protein Families Secreted Protein, Transmembrane
MW 82.4 kDa
Summary The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2014
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Documents
Resources
"NM_003460" in other vectors (6)
SKU Description Size Price
RC219115 ZP2 (Myc-DDK-tagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2) 10 ug
$682.00
RC219115L1 Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), Myc-DDK-tagged 10 ug
$982.00
RC219115L2 Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mGFP tagged 10 ug
$982.00
RC219115L4 Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mGFP tagged 10 ug
$982.00
RG219115 ZP2 (tGFP-tagged) - Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2) 10 ug
$789.00 $882.00
SC111059 ZP2 (untagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2) 10 ug
$683.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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