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JIP2 (MAPK8IP2) (NM_016431) Human Tagged ORF Clone

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SKU
RC219089
MAPK8IP2 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $730.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol JIP2
Synonyms IB2; JIP2; PRKM8IPL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219089 representing NM_016431
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCCCGATTTCCCTTCTCCTAGCACCTGGGCCCCTGGGCTACTGCTGCCCTCTGGCCCAGCCCTGC
TATCTCCCTCCGTCCTGCAGGACAGCCTCTCCCTGGGGCGCTCGGAGCAGCCGCACCCCATCTGCTCCTT
CCAGGATGACTTCCAGGAGTTTGAGATGATCGATGACAATGAAGAGGAGGACGATGAGGACGAGGAAGAG
GAGGAGGAGGAGGAGGAGGGAGATGGGGAAGGCCAGGAGGGAGGAGACCCTGGCTCAGAGGCACCTGCCC
CCGGGCCCCTTATCCCCTCCCCTTCCGTGGAGGAGCCCCACAAGCACCGGCCCACCACCCTCCGTCTGAC
CACACTGGGGGCCCAGGACTCCCTAAACAACAACGGAGGCTTTGACCTGGTGCGTCCGGCCTCCTGGCAG
GAGACAGCGCTATGCTCACCCGCCCCGGAGGCCCTCAGAGAGCTCCCGGGCCCCCTCCCTGCCACGGACA
CCGGGCCCGGCGGGGCGCAGTCGCCAGTGCGCCCGGGTTGCGACTGCGAAGGGAACCGGCCTGCGGAACC
CCCTGCGCCAGGGGGGACTTCGCCCTCCTCAGATCCCGGCATCGAGGCTGACCTGAGAAGCCGCTCGAGC
GGCGGCCGCGGGGGACGTCGCAGCAGCCAGGAGCTGTCCTCGCCCGGCTCCGACTCGGAGGACGCGGGCG
GCGCGCGCCTGGGGCGCATGATCTCGTCCATCTCGGAGACGGAGCTGGAGCTGAGCAGCGATGGCGGAAG
CAGCAGCAGCGGCCGCTCCTCGCACCTCACCAACTCCATCGAGGAGGCCTCGTCGCCCGCCTCGGAGCCG
GAGCCCCCGCGCGAACCCCCGCGCCGCCCCGCCTTCCTGCCCGTGGGCCCCGACGACACCAACAGCGAGT
ACGAGTCGGGGTCGGAGTCGGAGCCGGACCTCAGCGAGGACGCGGACTCGCCCTGGCTGCTCAGCAACCT
GGTGAGCCGCATGATCTCCGAGGGCTCCTCGCCCATCCGCTGCCCCGGCCAGTGCCTGTCTCCTGCGCCG
CGCCCGCCCGGGGAGCCCGTGTCGCCGGCCGGCGGGGCCGCCCAGGACTCCCAGGACCCCGAGGCGGCCG
CGGGGCCCGGCGGCGTGGAGCTGGTGGACATGGAGACGCTGTGCGCGCCGCCGCCGCCCGCGCCCGCCGC
GCCTCGACCCGGCCCCGCGCAGCCCGGGCCCTGCCTATTCCTCAGCAACCCCACGCGTGACACCATCACG
CCGCTGTGGGCCGCGCCCGGCCGCGCCGCCCGCCCGGGACGAGCCTGCTCCGCCGCCTGCTCCGAGGAGG
AGGACGAAGAGGACGACGAGGAAGAGGAGGATGCCGAGGACAGTGCGGGGTCCCCCGGGGGCAGGGGCAC
GGGCCCCTCGGCGCCGCGGGACGCGTCGCTGGTGTACGACGCGGTCAAGTACACGCTGGTGGTGGATGAG
CACACGCAGCTGGAGCTGGTGAGCCTGCGGCGCTGTGCTGGGCTGGGCCACGACAGCGAAGAGGACAGCG
GCGGGGAGGCCAGCGAGGAGGAGGCGGGCGCGGCGCTGCTAGGCGGCGGTCAGGTCTCGGGGGACACCTC
GCCGGACAGCCCTGACCTCACTTTCTCCAAGAAGTTCCTCAATGTCTTCGTCAACAGCACATCTCGGTCC
TCCAGCACCGAGTCCTTTGGCCTTTTCTCCTGTCTGGTCAACGGCGAGGAGCGAGAGCAGACTCACCGGG
CTGTGTTCAGGTTCATCCCGCGGCATCCAGACGAGCTGGAGCTGGATGTGGATGACCCTGTGTTGGTGGA
GGCCGAGGAGGACGACTTCTGGTTCCGTGGCTTCAACATGCGCACGGGGGAGCGCGGTGTGTTTCCTGCC
TTCTACGCCCATGCGGTGCCCGGCCCTGCCAAGGACCTGCTGGGGAGTAAGCGGAGCCCCTGCTGGGTGG
AGCGCTTTGACGTGCAGTTCCTGGGCTCCGTGGAGGTGCCCTGCCACCAGGGCAACGGCATCCTGTGTGC
AGCCATGCAGAAGATTGCCACTGCCCGGAAACTGACCGTCCACCTGCGCCCTCCTGCCTCCTGTGACCTC
GAGATCTCTCTTCGGGGGGTCAAGCTGAGTCTGAGCGGAGGAGGGCCCGAGTTCCAGCGCTGCAGCCATT
TCTTCCAGATGAAGAACATCTCCTTCTGCGGCTGCCATCCCCGCAACAGCTGCTATTTCGGCTTCATCAC
CAAACACCCCCTGCTGAGCCGCTTCGCCTGCCACGTCTTTGTCTCCCAGGAGTCCATGAGGCCGGTGGCG
CAGAGTGTGGGCCGCGCCTTCCTGGAGTACTACCAAGAGCACCTGGCGTACGCCTGCCCCACGGAGGACA
TCTACCTGGAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219089 representing NM_016431
Red=Cloning site Green=Tags(s)

MLPDFPSPSTWAPGLLLPSGPALLSPSVLQDSLSLGRSEQPHPICSFQDDFQEFEMIDDNEEEDDEDEEE
EEEEEEGDGEGQEGGDPGSEAPAPGPLIPSPSVEEPHKHRPTTLRLTTLGAQDSLNNNGGFDLVRPASWQ
ETALCSPAPEALRELPGPLPATDTGPGGAQSPVRPGCDCEGNRPAEPPAPGGTSPSSDPGIEADLRSRSS
GGRGGRRSSQELSSPGSDSEDAGGARLGRMISSISETELELSSDGGSSSSGRSSHLTNSIEEASSPASEP
EPPREPPRRPAFLPVGPDDTNSEYESGSESEPDLSEDADSPWLLSNLVSRMISEGSSPIRCPGQCLSPAP
RPPGEPVSPAGGAAQDSQDPEAAAGPGGVELVDMETLCAPPPPAPAAPRPGPAQPGPCLFLSNPTRDTIT
PLWAAPGRAARPGRACSAACSEEEDEEDDEEEEDAEDSAGSPGGRGTGPSAPRDASLVYDAVKYTLVVDE
HTQLELVSLRRCAGLGHDSEEDSGGEASEEEAGAALLGGGQVSGDTSPDSPDLTFSKKFLNVFVNSTSRS
SSTESFGLFSCLVNGEEREQTHRAVFRFIPRHPDELELDVDDPVLVEAEEDDFWFRGFNMRTGERGVFPA
FYAHAVPGPAKDLLGSKRSPCWVERFDVQFLGSVEVPCHQGNGILCAAMQKIATARKLTVHLRPPASCDL
EISLRGVKLSLSGGGPEFQRCSHFFQMKNISFCGCHPRNSCYFGFITKHPLLSRFACHVFVSQESMRPVA
QSVGRAFLEYYQEHLAYACPTEDIYLE

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_016431
ORF Size 2391 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016431.3, NP_057515.1
RefSeq Size 3216 bp
RefSeq ORF 2393 bp
Locus ID 23542
Cytogenetics 22q13.33
Protein Families Druggable Genome
Protein Pathways MAPK signaling pathway
MW 84.5 kDa
Summary This gene encodes a scaffold protein that is thought to be involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. This protein has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. provided by RefSeq, Jun 2017
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"NM_016431" in other vectors (6)
SKU Description Size Price
RC219089L1 Lenti ORF clone of Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,030.00
RC219089L2 Lenti ORF clone of Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2, mGFP tagged 10 ug
$1,030.00
RC219089L3 Lenti ORF clone of Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,030.00
RC219089L4 Lenti ORF clone of Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2, mGFP tagged 10 ug
$1,030.00
RG219089 MAPK8IP2 (tGFP-tagged) - Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2 10 ug
$930.00
SC304420 MAPK8IP2 (untagged)-Human mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2), transcript variant 2 10 ug
$804.00

Plasmid Map for RC219089

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219089
Circular map for RC219089

file-alt Citations

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